Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pseudomonas putida C2 for degrading unsymmetrical dimethylhydrazine and a method thereof for degrading unsymmetrical dimethylhydrazine

A technology of Pseudomonas putida and unsymmetrical dimethylhydrazine, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of insufficient tolerance of unsymmetrical dimethylhydrazine and chemical oxygen demand High concentration, unable to achieve the desired effect, etc., to achieve the effect of reduced harm, high speed, and large scale of treatment

Inactive Publication Date: 2014-03-26
CHONGQING UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main shortcoming of this method is: 1. can only handle the unsymmetrical dimethylhydrazine wastewater of low concentration (less than 50mg / L), for middle and high concentration wastewater, because the tolerance of sludge to unsymmetrical dimethylhydrazine is limited, can't reach desired effect; ② The concentration of chemical oxygen demand (COD) in the treated water is relatively high, which is highly harmful to the environment
In the prior art, also by enriching and cultivating Stenotrophomonas maltophilia M12, using its seed liquid to degrade unsymmetrical dimethylhydrazine at a certain stirring speed and temperature, the main disadvantages of this method are: 1. the processing scale is low, and the Poor practicability; ② Stenotrophomonas maltophilia M12 has insufficient tolerance to unsymmetrical dimethylhydrazine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas putida C2 for degrading unsymmetrical dimethylhydrazine and a method thereof for degrading unsymmetrical dimethylhydrazine
  • Pseudomonas putida C2 for degrading unsymmetrical dimethylhydrazine and a method thereof for degrading unsymmetrical dimethylhydrazine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Utilize Pseudomonas putida C2 to process the method for unsymmetrical dimethylhydrazine wastewater, concrete steps are as follows:

[0019] (1) Inoculate the strain of Pseudomonas putida C2 on the slant culture medium, and culture it at 20-30° C. for 10-12 hours to obtain the slant culture. The components of the above slant medium are: beef extract 3-5g / L, soybean meal 1-2g / L, maltose 0.5-1.5g / L, disodium hydrogen phosphate 1-2g / L, potassium dihydrogen phosphate 0.5-1.5g / L, ferrous sulfate 0.1~0.5g / L, agar 10~15g / L.

[0020] (2) Inoculate the above slant culture into an Erlenmeyer flask containing 1L of culture solution, place the Erlenmeyer flask in a biological shaker with a rotation speed of 100-200r / min and a temperature of 25-35°C, and shake for 18 ~24h to obtain the seed enrichment solution. The components of the above culture solution are: beef extract 3-5g / L, soybean meal 1-2g / L, maltose 0.5-1.5g / L, disodium hydrogen phosphate 1-2g / L, potassium dihydrogen pho...

Embodiment 2

[0024] Utilize Pseudomonas putida C2 to process the method for unsymmetrical dimethylhydrazine wastewater, concrete steps are as follows:

[0025] The difference between this embodiment and the first embodiment is: the seed enrichment solution of Pseudomonas putida C2 is added to the membrane bioreactor by the 2% (volume percentage) inoculum of the activated sludge in the membrane bioreactor In the activated sludge of the container, it is exposed for 26 hours to make it evenly mixed with the sludge. After 26 hours, the membrane bioreactor inoculated with Pseudomonas putida C2 and the common membrane bioreactor were both running continuously to treat 90mg / L unsymmetrical dimethylhydrazine wastewater. The seed enrichment solution of Pseudomonas putida C2 that was not inoculated in the activated sludge of ordinary membrane bioreactor was used as a blank reference. The rest are the same as those of the first embodiment, and will not be described in detail here.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a pseudomonas putida C2 for degrading unsymmetrical dimethylhydrazine and a method for degrading the unsymmetrical dimethylhydrazine by using the pseudomonas putida C2. The method comprises the steps of: inoculating the strain of the pseudomonas putida C2 to a slant culture medium and obtaining a slant culture through cultivation; inoculating the slant culture to a conical flask with a culture solution for culturing to obtain a seed enrichment solution; and feeding the seed enrichment solution into a membrane bioreactor, carrying out closed aeration for 24-32h, and then running the membrane bioreactor to treat unsymmetrical dimethylhydrazine wastewater. The removal rate of the pseudomonas putida C2 provided by the invention to the unsymmetrical dimethylhydrazine with the concentration of 50mg / L is 99.76%, and the removal rate of the pseudomonas putida C2 to the unsymmetrical dimethylhydrazine with the concentration of 90mg / L is 99.6%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for degrading unsymmetrical dimethylhydrazine by using Pseudomonas putida C2. Background technique [0002] Unsymmetrical dimethylhydrazine is a colorless and transparent liquid with a strong pungent fishy odor, and its chemical structure is (CH 3 ) 2 N-NH 2 , due to its high calorific value, high specific thrust, and good combustion performance, unsymmetrical dimethylhydrazine is also an excellent propellant. [0003] Unsymmetrical dimethylhydrazine is volatile and is a highly toxic substance with strong adsorption and genotoxicity. When the poisoning is severe, it will induce tumors in the body. At present, the extensive use of unsymmetrical dimethylhydrazine has had a serious impact on our country's environment and human health. In order to reduce the harm of unsymmetrical dimethylhydrazine, China Aerospace Industry Wastewater Discharge Standard (GB14374-93) stipulate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C02F3/34C12R1/40C02F101/38
Inventor 谭世语赵辉王力夏本立郭骅赵亚楠黄兆云刘又畅
Owner CHONGQING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com