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Method for producing dihydroxyacetone by converting glycerin by virtue of biological fermentation process

A technology of living dihydroxyacetone and biological fermentation method, which is applied in the biological field, can solve the problems of low glycerol concentration resistance of bacteria strains, bacterium cracking and inactivation process, and low product recovery rate, so as to increase product yield and improve the utilization of bacteria The effect of efficiency and process simplification

Inactive Publication Date: 2014-03-26
BEIJING GUANHONG TECH
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  • Abstract
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for producing dihydroxyacetone by fermentation, which can overcome the low concentration of glycerin resistance of bacterial strains in the current production process of dihydroxyacetone, the easy cracking and inactivation of bacterial cells by batch fermentation, and the existing problems in the subsequent separation and extraction process. Problems such as complex process, small separation capacity, high cost and low product recovery rate

Method used

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  • Method for producing dihydroxyacetone by converting glycerin by virtue of biological fermentation process

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Embodiment 1

[0020] (1) Inoculate high glycerol tolerance Portuguese Corynespora lusitaniae (Clavispora lusitaniae) bacterial strain G10 in the first stage reactor that adds sterilized fermentation medium, and fermentation medium is: yeast powder: 1%, peptone: 3%, ( NH 4 ) 2 SO 4 : 0.3%, NH 2 PO 4 : 0.05%, MgSO 4 : 0.01%, CaCO 3 : 0.05%, prepared with distilled water, adjusted the pH value to 7.0 with NaOH solution, and sterilized at 120°C and under high pressure.

[0021] (2) Add 5% sodium alginate solution and 2% CaCl to the bacterial suspension in the first-stage reactor 2 The solution is mixed evenly and flowed into the second-stage reactor, the temperature is 30-35°C, the rotation speed is 300r / min, the ventilation rate is 2vvm, and the pH value is about 5.0. Glycerin is fed at a speed of 15L / h for biocatalytic reaction. A pressure sensor is added to the outlet of the reactor, and when the glycerol concentration reaches 400g / L, the reaction liquid will flow out automatically. ...

Embodiment 2

[0025] (1) Inoculate high glycerol tolerance Portuguese Corynespora lusitaniae (Clavispora lusitaniae) bacterial strain G10 in the first stage reactor that adds sterilized fermentation medium, fermentation medium is: yeast powder: 4%, peptone: 1%, ( NH 4 ) 2 SO 4 : 0.1%, NH 2 PO 4 : 0.02%, MgSO 4 : 0.05%, CaCO 3 : 0.05%, prepared with distilled water, adjusted the pH value to 5.0 with NaOH solution, and sterilized at 120°C and autoclaved.

[0026] (2) Mix the bacterial suspension in the first stage reactor with 8% sodium alginate solution and 2% CaCl 2 The solutions are mixed and flowed into the second-stage reactor at a temperature of 30-35°C, a rotation speed of 300r / min, a ventilation rate of 2vvm, and a pH value of about 5.0. Glycerol is fed at a rate of 20L / h for biocatalytic reaction. In the second-stage reaction A pressure sensor is added to the outlet of the device, and when the concentration of glycerin reaches 400g / L, the reaction liquid will flow out automati...

Embodiment 3

[0030](1) Inoculate high glycerol tolerance Portuguese Corynespora lusitaniae (Clavispora lusitaniae) bacterial strain G10 in the first stage reactor that adds sterilized fermentation medium, and fermentation medium is: yeast powder: 6%, peptone: 4%, ( NH 4 ) 2 SO 4 : 0.1%, NH 2 PO 4 : 0.02%, MgSO 4 : 0.05%, CaCO 3 : 0.03%, prepared with distilled water, adjusted the pH value to 4.0 with NaOH solution, and sterilized at 120°C and under high pressure.

[0031] (2) Mix the bacterial suspension in the first-stage reactor with 10% sodium alginate solution and 4% CaCl 2 The solutions are mixed and flowed into the second-stage reactor at a temperature of 30-35°C, a rotational speed of 400r / min, a ventilation rate of 2vvm, and a pH value of about 5.0. Glycerin is fed at a rate of 25L / h for biocatalytic reaction. In the second-stage reaction A pressure sensor is added to the outlet of the device, and when the concentration of glycerin reaches 400g / L, the reaction liquid will fl...

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Abstract

The invention relates to a new method for producing dihydroxyacetone by converting glycerin by virtue of a biological fermentation process. The new method mainly comprises the following steps: producing dihydroxyacetone by fermenting a clavispora lusitaniae strain G10 with high glycerin resistance with two-order reactors and extracting dihydroxyacetone from the fermentation liquor. When the concentration of the glycerin substrate is 400g / L, the glycerin conversion rate is more than 85% and the yield is more than 75% after continuous fermentation is performed for 420 hours, so that the inhibiting effects of too high concentrations of the glycerin substrate of dihydroxyacetone prepared by existing microbiological fermentation methods and the product on the fermentation yield are effectively solved. The method adopted by the invention is simple to operate, is low in separation and extraction costs, is short in production time and is suitable for industrial production.

Description

technical field [0001] The invention relates to a new method for producing dihydroxyacetone, belonging to the field of biotechnology, in particular to a new method for producing dihydroxyacetone by oxidizing glycerol with glycerol dehydrogenase produced by fermentation of Clavispora lusitaniae strain G10. technical background [0002] Dihydroxyacetone is a naturally occurring ketose sugar, which is biodegradable, edible and non-toxic to the human body and the environment. Raw materials, food additives, and ingredients of cosmetic self-tanning agents can also be used as antiviral agents, and can be used for antiseptic preservation of fruits and vegetables, aquatic products, and meat products. [0003] Dihydroxyacetone is a natural product, which is difficult to extract and expensive, making it difficult to form a commercial scale. People use the structural characteristics of its three-carbon ketose to carry out chemical synthesis, but expensive catalysts are used, which make...

Claims

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Application Information

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IPC IPC(8): C12P7/26C12R1/645
Inventor 许波贺玉荣
Owner BEIJING GUANHONG TECH
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