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Water restoration agent for holothurian culture by employing attapulgite powder and rice husk as carriers, and preparation method and application of water restoration agent

A technology of attapulgite powder and repairing agent, which is applied in the field of aquaculture and marine microorganisms. It can solve the problems of unfavorable microbial agent reproduction, low dissolved oxygen at the bottom of the pond, and low purification rate of the upper and middle layers of water, achieving suspension and Good controllability of sinking and floating, prevention of sea cucumber diseases, and remarkable purification effect

Active Publication Date: 2014-04-02
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the micro-ecological water quality regulators used in aquaculture are limited to simple bacterial agents or carrier-based bacterial agents. The former has disadvantages such as poor adaptability to survival and reproduction of the bacterial flora and reduced number, while the latter is basically mostly deposited in the pond. The bottom, the purification rate of the middle and upper water body is low, and the dissolved oxygen in the bottom environment of the cutting pool is low, which is not conducive to the reproduction of micro-ecological agents

Method used

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  • Water restoration agent for holothurian culture by employing attapulgite powder and rice husk as carriers, and preparation method and application of water restoration agent

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Experimental program
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Effect test

Embodiment 1

[0035] 1. Preparation of probiotic stock solution

[0036] Bacillus subtilis YB-1 CGMCC No. 5116, Bacillus cereus YB-2 CGMCC No. 5117 and Bacillus flexus XH-1 CGMCC No. 5115, which have grown well on LB agar slant medium, were added to 2116E medium. , PH7.0~7.5, shaking at 37℃ for 12~16h, and then mixing at a volume ratio of 1:1:1 to prepare a probiotic stock solution, the cell concentration is about 1×10 8 cfu / ml.

[0037] 2. Liquid fermentation culture

[0038] The fermentation broth is added to the closed fermentation reactor, and nutrients are added at a volume ratio of 0.1 to 1%, and then the probiotic stock solution prepared in step ① is inoculated at a volume ratio of 3-5% for fermentation. The pressure is Fermented at 0.1~0.25Mpa at 37℃ for 24h to obtain the fermentation product;

[0039] The above nutrients are composed of corn meal: soybean meal meal: sea water in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0040] 3. Spray drying treatment

[0041] The adsorption carrier is ...

Embodiment 2

[0044] 1. Preparation of probiotic stock solution

[0045] Bacillus subtilis YB-1 CGMCC No. 5116, Bacillus cereus YB-2 CGMCC No. 5117 and Bacillus flexus XH-1 CGMCC No. 5115, which have grown well on LB agar slant medium, were added to 2116E medium. , PH7.0-7.5, shaking at 37°C for 12-16h, and then mixing at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0046] 2. Liquid fermentation culture

[0047] The fermentation broth is added to the closed fermentation reactor, and nutrients are added at a volume ratio of 0.1 to 1%, and then the probiotic stock solution prepared in step ① is inoculated at a volume ratio of 3-5% for fermentation. The pressure is Fermented at 0.1~0.25Mpa at 37℃ for 24h to obtain the fermentation product;

[0048] The above nutrients are composed of corn meal: soybean meal meal: sea water in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0049] 3. Spray drying treatment

[0050] The adsorption carrier is...

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Abstract

The invention discloses a water restoration agent for holothurian culture by employing attapulgite powder and a rice husk as carriers, and a preparation method and application of the water restoration agent. The water restoration agent is prepared from bacillus flexus CGMCC No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117 separated and screened from a holothurian culture environment by applying a marine microorganisms technology according to the physical habits and the breeding characteristics of holothurians. The method comprises the following steps: fermenting for 24-48 hours at 28-37 DEG C by taking corn powder and soybean meal as main composts, and then processing by taking the attapulgite powder and the rice husk as adsorption and suspension carriers, so as to prepare the water restoration agent for holothurian culture by employing the attapulgite powder and the rice husk as the carriers. The water restoration agent is strong in tolerance on a water environment, and good in floatability, suspension property and up and down controllability, a three-dimensional purification effect of seawater aquaculture water is facilitated, hazardous substances of heavy metal ions such as Hg<2+>, Pb<2+>, Cr<6+>, Cd<2+> and Cu<2+> in water can be adsorbed, pathogenic microorganisms are effectively inhibited, a holothurian disease is prevented, and the yield and the quality of holothurian culture are improved.

Description

Technical field [0001] The invention is in the technical field of aquaculture and marine microorganisms, and particularly relates to a sea cucumber aquaculture water quality repair agent with attapulgite powder and rice husk as carriers, and a preparation method and application thereof. Background technique [0002] Sea water is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water." The quality of water directly affects the growth of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people’s demand for sea cucumbers is increasing. Under the condition that natural fishing cannot meet the market demand, the sea cucumber aquaculture industry has transformed from the traditional extensive management and resource-dependent aquaculture to the current intensive and factory The culture of sea cucumber has become one of the pillar indus...

Claims

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Application Information

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IPC IPC(8): C02F3/34A01K61/00
CPCY02A40/81
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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