Composite carrier micro-ecologic water purification agent for holothurian culture and preparation method and application thereof

A technology of composite carrier and purifying agent, applied in the field of aquaculture and marine microorganisms, can solve the problems of excessive antibiotic residues, aquaculture water pollution, high mortality of sea cucumbers, etc., and achieve a significant purification effect, avoid acidification and odor, and reduce stress. effect of reaction

Active Publication Date: 2014-04-30
DALIAN SEM BIOLOGICAL ENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the current situation in the prior art, such as serious pollution of breeding water quality, frequent occurrence of diseases, high mortality rate of sea cucumbers, excessive heavy metals, chemical residues and antibiotic residues in sea cucumbers, etc., the present invention invents a composite carrier micro-ecological water quality purifier for sea cucumber cultivation and its preparation and use

Method used

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  • Composite carrier micro-ecologic water purification agent for holothurian culture and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of probiotic stock solution

[0038] Bacillus subtilis YB-1CGMCC No.5116, Bacillus cereus YB-2CGMCC No.5117 and Bacillus flexus XH-1CGMCC No.5115, which grew well on LB agar slant medium, were added to 2116E medium, pH7. 0~7.5, shake culture at 37℃ for 12~16h, then mix with volume ratio of 1:1:1 to prepare probiotic stock solution, the concentration of bacteria is about 1×10 8 cfu / ml.

[0039] 2. Liquid fermentation culture

[0040] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0041] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0042] 3. Spray drying treatment

[0043] The composite adsorption carri...

Embodiment 2

[0046] 1. Preparation of probiotic stock solution

[0047] Bacillus subtilis YB-1CGMCC No.5116, Bacillus cereus YB-2CGMCC No.5117 and Bacillus flexus XH-1CGMCC No.5115, which grew well on LB agar slant medium, were added to 2116E medium, pH7. 0~7.5, shake culture at 37°C for 12~16h, and then mix at a volume ratio of 1:1:1 to prepare a probiotic stock solution with a cell concentration of about 1×108cfu / ml.

[0048] 2. Liquid fermentation culture

[0049] Add fermentation broth to the closed fermentation reactor, and add nutrients at a volume ratio of 0.1 to 1%, then inoculate the probiotic stock solution prepared in step ① at a volume ratio of 3-5%, and carry out fermentation at a pressure of 0.1~0.25Mpa, ferment at 37°C for 24 hours to obtain the fermentation product;

[0050] The above nutrients are composed of corn flour: soybean meal flour: seawater in a weight ratio of (1.5-3): (0.5-1.5): 100.

[0051] 3. Spray drying treatment

[0052] The adsorption carrier is prepa...

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Abstract

The invention discloses a composite carrier micro-ecologic water purification agent for holothurian culture, and a preparation method and application of the agent. Based on the physiologic properties and culture characteristics of holothurian, by virtue of a marine microorganism technique, the agent is composed of bacillus flexus CGMCCNO.5115, bacillus subtilis CGMCCNO.5116 and bacillus cereus CGMCCNo.5117 separated and screened from a holothurian culture environment. The preparation method comprises the following steps: with corn flour and soybean meal as main culture raw materials, fermenting for 24-48 hours at 28-37 DEG C; and finally processing in the presence of medical stone, sepiolite and rice husk serving as an adsorption and suspension composite carrier, thus obtaining the composite carrier micro-ecologic water purification agent. The agent has good water environment tolerance, good floatability, suspension property and sinking and floating controllability and is beneficial to purification effect on seawater culture water in a three-dimensional manner, the water is prevented from acidification, smelly water bottom is avoided, pathogenic microorganisms are effectively inhibited, and the yield and the quality of holothurian culture are improved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and marine microorganisms, and specifically relates to a composite carrier micro-ecological water quality purifier for sea cucumber cultivation and its preparation method and application. Background technique [0002] Seawater is the basic environmental condition for the survival of sea cucumbers and other sea treasures. As the saying goes: "Three points for fish, seven points for water". The quality of water quality directly affects the growth status of sea cucumbers and the economic benefits of farmers. With the development of economy and science, people's demand for sea cucumbers is increasing day by day. Under the circumstances that natural fishing cannot meet the market demand, the sea cucumber farming industry has transformed from the traditional extensive management and resource-dependent farming methods to the current intensive and factory farming methods. With the modernization of fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12N11/14A01K61/00C12R1/07C12R1/085C12R1/125
CPCY02A40/81
Inventor 徐永平尤建嵩李淑英李纪彬周俊龙徐牧马欣高玉平刘士茹
Owner DALIAN SEM BIOLOGICAL ENG TECH
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