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Ganoderma lucidum spore polysaccharide, preparation method and application

A technology of Ganoderma lucidum spores and Ganoderma lucidum spore powder, applied in the fields of application, food preparation, food science, etc., can solve the problems affecting industrialization and limiting the amplification of microwave devices, and achieve the effect of stable quality, simple method, and good water solubility

Active Publication Date: 2015-11-18
SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lv Long and others reported "Ganoderma lucidum spore powder polysaccharide, production method and application" [ZL03141851.1], the polysaccharide of Ganoderma lucidum spore is composed of 11 β-1,3 linked glucose as the main chain, and there are two β- 1,3,6 connect the glucose branch, the two branches are →1)-β-Glcp-(6→1)-α-Glcp and →1)-β-Glcp-(6→1)-α-Glcp -(4→1)-α-Glcp, the molecular weight range is 5000-11000Da, and the invention uses microwave softening treatment to obtain Ganoderma lucidum spore polysaccharide. In actual production, due to the penetrability of microwaves, the amplification of microwave devices is limited, affecting Therefore, a method that can replace microwave treatment to obtain Ganoderma lucidum spore polysaccharide has more application prospects

Method used

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  • Ganoderma lucidum spore polysaccharide, preparation method and application
  • Ganoderma lucidum spore polysaccharide, preparation method and application
  • Ganoderma lucidum spore polysaccharide, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0050] (1) Take 50 g of Ganoderma lucidum spore powder and add it to 500 mL of 0.1 mol / L aqueous sulfuric acid solution and stir, heat the sulfuric acid solution to 100°C and stir for 4 hours, add barium hydroxide to neutralize to pH 7.5 after the reaction, and evaporate the solvent to dryness under reduced pressure to obtain Acid-treated spore powder.

[0051] (2) Add 500 mL of water to the acid-treated spore powder in step (1) and soak for 2 hours, filter and concentrate the soaking liquid to 50 mL, add 150 mL of ethanol for ethanol precipitation, dissolve the precipitate in water, and centrifuge to take the supernatant, concentrate and dry to obtain Ganoderma lucidum spore polysaccharide.

[0052] (3) The solubility of the Ganoderma lucidum spore polysaccharide in water at room temperature is 90 mg / mL, and the solution is clear. Its molecular weight distribution is 850-9000 Da detected by high performance liquid phase, and the part with molecular weight of 2000-4900 Da acco...

Embodiment 2

[0058] (1) Take 50g of Ganoderma lucidum spore powder and add it to 62.5mL of 2mol / L phosphoric acid aqueous solution and stir. Heat the phosphoric acid solution to 60°C and stir for 10 minutes. After the reaction is completed, add sodium hydroxide to neutralize to pH 7.0, evaporate the solvent to dryness under reduced pressure to obtain acid Dispose of spore powder.

[0059] (2) Add 750 mL of water to the acid-treated spore powder in step (1) and soak for 6 hours, filter and concentrate the soaking liquid to 60 mL, add 300 mL of acetone to ethanol, dissolve the precipitate in water, centrifuge, take the supernatant, concentrate and dry to obtain Ganoderma lucidum spore polysaccharide. Its molecular weight distribution is 750-9000 Da detected by high-performance liquid phase, and the part with molecular weight of 2000-4900 Da accounts for 77%.

Embodiment 3

[0061] (1) Take 50 g of Ganoderma lucidum spore powder and add it to 150 mL of 1mol / L hydrochloric acid aqueous solution and stir. Heat the hydrochloric acid solution to 80° C. and stir for 30 minutes. After the reaction, add calcium carbonate to neutralize to pH 6.0, evaporate the solvent to dryness under reduced pressure to obtain acid-treated spores pink.

[0062] (2) Add 400 mL of water to the acid-treated spore powder in step (1) and soak for 12 hours, filter and concentrate the soaking liquid to 60 mL, add 480 mL of methanol for ethanol precipitation, dissolve the precipitate in water, centrifuge, take the supernatant, concentrate and dry to obtain Ganoderma lucidum spore polysaccharide. Its molecular weight distribution is 700-10000 Da detected by high-performance liquid phase, and the part with molecular weight of 2000-4900 Da accounts for 65%.

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Abstract

The invention relates to a ganoderma lucidum spore polysaccharide and a preparation method and application thereof. The ganoderma lucidum spore polysaccharide is water soluble glucan, the molecular weight is within the range of 700-10000Da, and the part of which the molecular weight is 2000-4900Da is 60-85%. The method comprises the following steps: carrying out thermal treatment on ganoderma lucidum spore powder by using an inorganic acid or organic acid solution; removing the solvent in a vacuum distillation manner, and then soaking the ganoderma lucidum spore powder by water; filtering the soaking liquid and adding an organic solvent to participate; dissolving sediments by water; centrifuging and taking supernate to concentrate and dry, so as to obtain the ganoderma lucidum spore polysaccharide. The ganoderma lucidum spore polysaccharide can be used for preparing a drug or a health-care product with the functions of resisting tumors and enhancing the immunity.

Description

field of invention [0001] The invention relates to a ganoderma lucidum spore polysaccharide, a preparation method and an application. The water-soluble ganoderma lucidum spore polysaccharide is a kind of dextran with a molecular weight of 700-10000 Da, and the part with a molecular weight of 2000-4900 Da accounts for 60-85%. technical background [0002] Ganoderma lucidum spores are the spores of Chizhi or Zizhi of the family Polyporaceae Basidiomycetes. Ganoderma lucidum spores are brown oval, one end is truncated, the size is (8.5-11.2) μm×(5.2-6.9) μm, and it has a double-walled structure. The chemical components of Ganoderma lucidum spores mainly include the following categories: proteins and amino acids, glycopeptides, vitamins, carotene, sterols, triterpenes, alkaloids, fatty acids, lactones and inorganic ions. Ganoderma lucidum spores are rich in polysaccharides and oligosaccharides, but due to its tough double-wall structure, the active ingredients in the spores ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00A61P37/04A23L1/09A23L33/125
Inventor 吕龙江艳杨佳琰曹义苗项玉华丁志刚
Owner SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI