Colorimetric detection method of hydrogen peroxide

A hydrogen peroxide and detection method technology, which is applied in the direction of material analysis by observing the influence on chemical indicators, color/spectral characteristic measurement, and analysis by making materials undergo chemical reactions, can solve the problem of complex detection process and detection cost. High, high operating requirements, etc., to achieve the effect of low detection cost, good reproducibility, and mild enzyme-catalyzed reaction conditions

Active Publication Date: 2014-04-30
FUZHOU UNIVERSITY
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  • Abstract
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Problems solved by technology

[0005] The object of the present invention is to provide a kind of colorimetric detection method of hydrogen peroxide, utilizes molybdenum disulfide as catalyst to catalyze the color reaction of hydrogen peroxide and color developer to detect hydrogen peroxide, can solve the problem of peroxide in the prior art. The o

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  • Colorimetric detection method of hydrogen peroxide

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Embodiment 1

[0024] Detection of hydrogen peroxide in aqueous solution: Mix 0.2 mL hydrogen peroxide sample solution with 0.05 mL molybdenum disulfide solution (18 mg / L), 0.05 mL o-phenylenediamine (0.3 M) and 0.2 mL buffer solution (10 mM , pH 1) mixed, 12 minutes after the reaction can produce obvious color changes, semi-quantitative analysis can be carried out by visual colorimetry according to the color changes, or quantitative analysis can be carried out by measuring the absorbance at 450 nm wavelength by UV-spectrophotometry.

[0025] Standard colorimetric series: Mix 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL of o-phenylenediamine (0.3 M) and 0.2 mL of buffer solution (10 mM, pH 1), and then add 0.2 mL of hydrogen peroxide standard solution of different concentrations, the concentrations are 0 μM, 5 μM, 20 μM, 40 μM, 60 μM, 80 μM, 100 μM, 150 μM, 200 μM, mix well, and react at 60°C for 30 min directly as a colorimetric standard series.

Embodiment 2

[0027] Detection of hydrogen peroxide in aqueous solution: mix 0.2 mL hydrogen peroxide sample solution with 0.05 mL molybdenum disulfide solution (18 mg / L), 0.05 mL 2,2-azino-bis(3-ethyl-benzo Thiazole-6-sulfonic acid) diammonium salt (20 mM) and 0.2 mL buffer solution (10 mM, pH 1) are mixed, and an obvious color change can be produced 12 minutes after the reaction, which can be determined by visual colorimetry according to the color change Semi-quantitative analysis, or quantitative analysis by measuring the absorbance at a wavelength of 405 nm by UV-spectrophotometry.

[0028] Standard colorimetric series: Mix 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL of 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (20 mM) and 0.2 mL buffer solution (10 mM, pH 1), after mixing, add 0.2 mL of hydrogen peroxide standard solution of different concentrations, the concentrations are 0 μM, 5 μM, 20 μM, 40 μM, 60 μM , 80 μM, 100 μM, 150 μM, and 200 μM we...

Embodiment 3

[0030] Detection of hydrogen peroxide in aqueous solution: mix 0.2 mL hydrogen peroxide sample solution with 0.05 mL molybdenum disulfide solution (18 mg / L), 0.05 mL 3,3',5,5'-tetramethylbenzidine ( 12 mM) mixed with 0.2 mL buffer solution (10 mM, pH 6.9), a significant color change can be produced 12 minutes after the reaction, which can be semi-quantitatively analyzed by visual colorimetry according to the color change, or by UV-spectrophotometry Quantitative analysis was carried out by measuring the absorbance at a wavelength of 652 nm; or adding 10 μL of 20% (V / V) sulfuric acid solution to terminate the reaction, and performing semi-quantitative analysis by visual colorimetry or using UV-spectrophotometry at a wavelength of 450 nm Absorbance was measured for quantitative analysis.

[0031]Standard colorimetric series: 0.05 mL of molybdenum disulfide solution (18 mg / L), 0.05 mL of 3,3',5,5'-tetramethylbenzidine (12 mM) and 0.2 mL of buffer solution (10 mM, pH 6.9), after m...

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Abstract

The invention discloses a colorimetric detection method of hydrogen peroxide. The colorimetric detection method comprises the following steps: mixing a sample solution containing hydrogen peroxide with a molybdenum disulfide solution, a color developing agent and a buffer solution, and measuring the content of the hydrogen peroxide in the sample by adopting a visual colorimetry or an ultraviolet-visible spectrophotometer after reaction. According to the colorimetric detection method, the chromogenic reaction of the hydrogen peroxide and the color developing agent is catalyzed by using molybdenum disulfide as a catalyst to detect the hydrogen peroxide, and the concentration of the hydrogen peroxide is semi-quantitatively determined by adopting a visual colorimetry, or the concentration of the hydrogen peroxide is quantitatively determined by adopting an ultraviolet spectrophotometer. According to the colorimetric detection method, the problems of high operation requirement for detection of the hydrogen peroxide, complicated detection process, high detection cost, long detection time, large background interference and the like in the prior art can be solved. The colorimetric detection method disclosed by the invention is low in cost, simple and convenient to operate, and capable of realizing visible quick detection of the hydrogen peroxide.

Description

technical field [0001] The invention belongs to the technical field of hydrogen peroxide detection, and in particular relates to a colorimetric detection method of hydrogen peroxide. Background technique [0002] Hydrogen peroxide is commonly known as hydrogen peroxide, and its chemical formula is H 2 o 2 , often used in food processing, but because long-term consumption of foods containing hydrogen peroxide may cause cancer, the state strictly prohibits hydrogen peroxide residues in the final food products, and only a few foods allow maximum residues, such as dried tofu. [0003] The current national standards for the detection of hydrogen peroxide (GB / T 23499-2009 Determination of residual hydrogen peroxide in food, GB 1616-2003 Industrial hydrogen peroxide), mostly based on potassium permanganate, titanium salt or potassium iodide The color change produced by the oxidation-reduction reaction is used as the end point of the titration, and the content of hydrogen peroxide...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N21/33
Inventor 郭良洽林天然
Owner FUZHOU UNIVERSITY
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