Cell microscopic imaging method, image processing method and imaging analysis system

Abstract

The invention relates to a cell micro-imaging method, an image processing method and an imaging analysis system. The cell micro-imaging method is implemented by using a soft X ray and comprises the following steps of (1) preparing a sample; (2) primarily seeking and selecting a target cell; and (3) imaging the soft X ray. The image processing method is capable of reducing noises, eliminating artifacts and improving the contrast ratio. The imaging analysis system comprises an optical microscope system, a synchrotron radiation device, an image processing system and a display device. The methods and the system provided by the invention have the advantages that the existence and the region environment of a cell are effectively detected, the space scales, the structures and the functions of the cell and a sub-cell are researched, and thus the effective middle information is provided for multiple fields such as pathologies, medicines, agriculture and forestry and foods.

Description

technical field [0001] The invention relates to a method for microscopic imaging of cells by using soft X-rays, an image processing method and an imaging analysis system. Background technique [0002] X-rays discovered by Roentgen in 1895 have the property of strongly penetrating matter and are suitable for imaging human tissues and organs. Its wavelength is shorter, 0.1-1.0nm. However, the interaction between X-rays and matter is very complicated, and it is difficult to manufacture optical components similar to those used in visible light applications, so it is difficult to realize X-ray microscopes. With the birth of the electron collision synchrotron radiation source, special soft X-rays are emitted with a wavelength of 1-10nm. The soft X-rays are focused and projected onto the sample through optical diffraction and optical elements, and then imaged on the image plane. [0003] The resolution of soft X-ray microscopy has reached 10nm, the principle is: [0004] The sam...

AI Technical Summary

Problems solved by technology

[0011] In China, at present, soft X-ray microscopic imaging technology is mostly used in microbiology, plant and animal biological sample specimens, and the use of soft X-ray for cell microscopic imaging research is still in its infancy and initial stage, and the research focus is mainly on In terms of soft X-ray imaging operations, too much reliance on X-ray imaging itself, less research on sample processing and image processing, the impact of sample processing and image processing on image information is often ignored, and there is still a lack of research on the best suitable for Research on Diffraction Grating for Soft X-ray Imaging

In addition, due to the influence of traditional X-ray imaging techniques (such as hard X-ray imaging technology), less attention is paid to the folds that may occur during ultrathin sectioning. Microscopic imaging of cells can have a great impact; in the imaging process, traditional X-ray imaging technology is often used, and there is a lack of research on the best grating and photoresist for soft X-rays, resulting in the performance of diffraction gratings being affected. limit

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