Fluorescent nano carbon point

Abstract

The invention discloses a fluorescent nano carbon point which takes a light-emitting bacterium as a carbon source and is prepared by using a method comprising the following steps: centrifugally collecting the light-emitting bacterium, putting into a mixture solution of water and 95% ethanol, pouring the mixture solution into a reaction kettle with a lining made of polytetrafluoroethylene for reaction, cooling down, subsequently pouring out the reaction solution, filtering, dialyzing the filtrate in a dialysis bag by using super pure water, changing the water every day in the period, filtering the solution in the dialysis bag by using a filtering film so as to obtain the filtrate, that is, the fluorescent nano carbon point. The carbon point disclosed by the invention is wide in raw material source, low in price and simple in preparation method; the carbon point is good in water solubility self, is not caked or agglomerated in water and is applicable to fluorescence imaging. The carbon point self also emits wavelength like a quantum dot and is also in red shift along with red shift of the excited wavelength. The bacterium which is a variety widely existing in living is applied to synthesis for the first time, and meanwhile the method can be expanded to other bacteria. The fluorescent nano carbon point disclosed by the invention can be applied to biologic imaging, biomarking and catalysis.

Description

technical field [0001] The invention relates to the preparation of fluorescent nanometer materials, in particular to fluorescent nanometer carbon dots prepared from novel materials and their application. Background technique [0002] At present, there are many kinds of fluorescent nanomaterials, such as: semiconductor fluorescent quantum dots (document 1: Empedocles S, Bawendi M, Acc. Chem. Res. 1999, 32, 389), polymer fluorescent nanospheres (document 2: Liu Q H, Liu J , Guo J C, J. Mater. Chem., 2009, 19, 2018), silica fluorescent material (document 3: Santra S, Zhang P, Wang K, et al, Anal. Chem. 2011, 73, 4988). However, semiconductor fluorescent quantum dots are still widely used, and their fluorescence peaks can be adjusted by changing the particle size and composition of quantum dots. However, due to the heavy metals contained in quantum dots, they are highly toxic, and have disadvantages such as unstable luminescence and easy flickering, which limit their applicatio...

AI Technical Summary

Problems solved by technology

[0007] However, there are some disadvantages in the above methods, such as high-temperature and high-pressure preparation of nanomaterials by laser method, expensive equipment, difficult conditions to control, and the electrolyte used in the electrochemical method has a great influence on whether fluorescence is generated, and the fluorescent quantum of the prepared fluorescent nanomaterials low yield

At the same time, there are also some deficiencies in terms of raw materials. The raw materials are relatively single, basically carbon nanotubes, graphite, activated carbon, etc. In addition, it is difficult to enrich the soot of incomplete combustion like candles.

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