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Method for extracting chlorogenic acid from honeysuckle

A technology of honeysuckle and chlorogenic acid, applied in the separation/purification of carboxylate, organic chemistry, etc., to achieve the effects of high yield, reduced industrial energy consumption, and simple operation

Active Publication Date: 2014-06-18
山东惠普生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of the chlorogenic acid extraction method in the prior art, the present invention provides a method for extracting chlorogenic acid from honeysuckle, which has high chlorogenic acid yield, low energy consumption, simple steps and easy industrial production

Method used

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  • Method for extracting chlorogenic acid from honeysuckle
  • Method for extracting chlorogenic acid from honeysuckle
  • Method for extracting chlorogenic acid from honeysuckle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting chlorogenic acid from Flos Lonicerae, comprising the steps of:

[0020] 1) Pulverization: put 1 kg of honeysuckle (produced in Pingyi, Shandong) into a pulverizer to pulverize, and then spread it into 1 square meter;

[0021] 2) Ultraviolet irradiation: irradiate under ultraviolet light for 10 minutes, and the ultraviolet intensity is 2000uW / cm 2 ;

[0022] 3) Hydrolysis: use deionized water 8 times the weight of honeysuckle as a solvent, maintain at 0.1 atmosphere, hydrolyze at 75°C for 1 hour, and collect the filtrate;

[0023] 4) concentration: the filtrate is placed in a vacuum concentrator, and vacuum concentrated to one-third of the volume of the filtrate;

[0024] 5) Precipitation: Add the calculated amount of alcohol so that the alcohol content of the concentrated solution reaches 75% (v / v). After standing for 1 hour, the chlorogenic acid is completely precipitated, and the filtrate is collected by filtration for extracting hedera sapon...

Embodiment 2

[0026] A method for extracting chlorogenic acid from Flos Lonicerae, comprising the steps of:

[0027] 1) Pulverization: put 1 kg of honeysuckle (produced in Pingyi, Shandong) into a pulverizer and pulverize it, and spread it into 1 square meter;

[0028] 2) UV irradiation: UV irradiation for 15 minutes, the UV intensity is 2000uW / cm 2 ;

[0029] 3) Hydrolysis: use deionized water 10 times the weight of honeysuckle as a solvent, maintain a state of 0.1 atmosphere, hydrolyze at 75°C for 1 hour, and collect the filtrate;

[0030] 4) concentration: the filtrate is placed in a vacuum concentrator, and vacuum concentrated to one-third of the volume of the filtrate;

[0031] 5) Precipitation: Add the calculated amount of alcohol so that the alcohol content of the concentrated solution reaches 75% (v / v). After standing for 1 hour, the chlorogenic acid is completely precipitated, and the filtrate is collected by filtration for extracting hedera saponin. After vacuum drying at 80°C,...

Embodiment 3

[0033] Effects of Factors on Extraction Efficiency of Chlorogenic Acid

[0034] 1. The influence of ultraviolet irradiation intensity and time, control other conditions unchanged, only change the ultraviolet irradiation parameters, repeat the experiment three times for each group, see Table 1 for specific results

[0035] Table 1

[0036]

[0037] Conclusion: Through analysis, it is found that ultraviolet irradiation can greatly increase the production of chlorogenic acid, but with the increase of time, the purity of chlorogenic acid is greatly reduced, which may be due to the decomposition of chlorogenic acid caused by strong light.

[0038] 2. The effect of hydrolysis time on the yield of chlorogenic acid

[0039] Change the time of hydrolysis, control other conditions unchanged, repeat each group of experiments three times, see Table 2 for specific results

[0040] Table 2

[0041]

[0042]

[0043] Conclusion: Through the analysis, it was found that after one h...

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Abstract

The invention relates to a method for extracting chlorogenic acid from honeysuckle. The method comprises the steps of grinding, ultraviolet radiation, hydrolysis, concentration, precipitation and the like. The method adopts ultraviolet with proper intensity for radiation and deionized water for hydrolysis and avoids using various extraction reagents and decoloring agents. The chlorogenic acid prepared by the method has high yield and the method has the advantages of low energy consumption and simple steps and can be widely applied to industrial production.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a method for extracting chlorogenic acid from honeysuckle. Background technique [0002] Chlorogenic acid (Chlorogenic acid), also known as chlorogenic acid or coffee tannin, system name 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid-(3,4-dihydroxycinnamate), is a plant A phenylpropanoid compound synthesized in the process of aerobic respiration, mainly derived from Chinese herbal medicines such as honeysuckle, Eucommia, and capillary. The hydrate of chlorogenic acid is a needle-like crystal, which can be transformed into an anhydrous compound at 110°C, with a melting point of 208°C, a solubility of 4% in water at 25°C, and a greater solubility in hot water; it is easily soluble in ethanol and acetone , very slightly soluble in ethyl acetate, hardly soluble in lipophilic organic solvents such as chloroform, ether, and benzene. Chlorogenic acid is an ester formed by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
CPCC07C67/48C07C2601/14C07C69/732
Inventor 石宏廉吉芳张宗桥庞朝征
Owner 山东惠普生物科技有限公司
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