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Method for preparing bacterial cellulose compounded amnion extracellular matrix material containing collagen

A technology of bacterial cellulose and collagen, applied in the methods of using microorganisms, catheters, medical science, etc., can solve the problems of easy folds and tears, poor biomechanical properties, difficult to stratify tissue, etc., and achieve a good spatial three-dimensional network structure. , the preparation process is simple and easy, the effect of a wide range of materials

Active Publication Date: 2015-06-10
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the amniotic membrane is thin and soft, it is difficult to construct multi-layered tissue. At the same time, the biomechanical properties are poor, and it is easy to fold and tear during use, which makes the operation difficult. Moreover, the amniotic membrane after transplantation is easy to fall off and dissolve, and the effect period is short. making it limited in tissue reconstruction

Method used

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  • Method for preparing bacterial cellulose compounded amnion extracellular matrix material containing collagen

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Effect test

Embodiment 1

[0022]Acetobacter xylinum, which can secrete bacterial cellulose, was selected to prepare seed mash with a strain concentration of 30%. The collagen was dissolved in 1wt% acetic acid aqueous solution and mixed with the medium to obtain a fermentation medium containing 0.5wt% collagen. Mix the seed mash with the collagen-containing fermentation medium and place it in a culture container. The liquid level of the mixed solution in the culture container is 0.5 cm, and culture it for 1 day to obtain hydrogel-like bacterial fibers with a film thickness of 0.5 cm. Vein / Collagen Membrane.

[0023] The amniotic membrane extracellular matrix material is taken from a safe source, processed to remove cells, fat and soluble antigens, and dried and sterilized, soaked in the culture medium, and spread on the upper surface of the bacterial cellulose / collagen membrane after it is completely wet. Wherein, the sponge layer of the amnion extracellular matrix material is in contact with the upper...

Embodiment 2

[0025] Rhizobia and Pseudomonas, which can secrete bacterial cellulose, were selected and activated to prepare seed mash with a strain concentration of 40wt%. The collagen was dissolved in 1.5wt% acetic acid aqueous solution and mixed with the medium to obtain a fermentation medium containing 1wt% collagen. Mix the seed mash with the collagen-containing fermentation medium and place it in a culture container. The height of the mixed solution in the culture container is 1 cm, and culture it for 2 days to obtain hydrogel-like bacterial cellulose with a film thickness of 0.6 cm. / collagen membrane.

[0026] The amniotic membrane extracellular matrix material is taken from a safe source, processed to remove cells, fat and soluble antigens, and dried and sterilized, soaked in the culture medium, and spread on the upper surface of the bacterial cellulose / collagen membrane after it is completely wet. Wherein, the sponge layer of the amnion extracellular matrix material is in contact...

Embodiment 3

[0028] The seed mash with a strain concentration of 50wt% was prepared by activation of Sarcina and Alcaligenes that could secrete bacterial cellulose. The collagen was dissolved in 2wt% acetic acid aqueous solution and mixed with the medium to obtain a fermentation medium containing 2wt% collagen. After the seed mash is mixed with the fermentation medium containing collagen, it is placed in a culture container, the liquid level of the mixed solution in the culture container is 2 cm, and the hydrogel-like bacterial cellulose / Collagen membrane.

[0029] The amniotic membrane extracellular matrix material is taken from a safe source, processed to remove cells, fat and soluble antigens, and dried and sterilized, soaked in the culture medium, and spread on the upper surface of the bacterial cellulose / collagen membrane after it is completely wet. Wherein, the sponge layer of the amnion extracellular matrix material is in contact with the upper surface of the bacterial cellulose / c...

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Abstract

The invention discloses a method for preparing a bacterial cellulose compounded amnion extracellular matrix material containing collagen, and relates to a preparation technology of tissue engineering biomaterials. The method comprises the following steps: activating a strain capable of secreting bacterial cellulose to prepare a seed mash; mixing the seed mash with strain concentration of 30-50wt% and a culture medium containing a collagen solution, and putting the mixture into a culture container to stand and culture to obtain a bacterial cellulose / collagen film; immersing an amnion extracellular matrix film in the culture medium, laying the amnion extracellular matrix film on the surface of the bacterial cellulose / collagen film after the amnion extracellular matrix film is completely wet; further standing and culturing for 1-6 hours, purifying and drying to obtain the bacterial cellulose compounded amnion extracellular matrix material containing collagen. The method is simple and practical in preparation process, low in cost and wide in material sources; the prepared compound material has an excellent spatial three-dimensional network, good biocompatibility and high mechanical strength, and can be used as a tissue engineering scaffold material and applied to tissue reconstruction.

Description

technical field [0001] The invention relates to the field of tissue engineering biomaterials, in particular to a preparation method of a collagen-containing bacterial cellulose composite amnion extracellular matrix material. Background technique [0002] The amnion is derived from the cytotrophoblast, the inner layer of the two membranes. The normal amniotic membrane is thin and clear, without blood vessels, nerves and lymphatic vessels, and its thickness is 20-500 μm. The amniotic membrane has 5 layers and can be divided into: epithelial layer, basement membrane layer, compact layer, fibroblast layer, and spongy layer. The amnion extracellular matrix material after cell removal contains various components such as collagen, glycoprotein, proteoglycan, integrin and lamellar body, and expresses a variety of growth factors and their mRNA related proteins, which can provide support for cell proliferation and differentiation. Rich nutrients are beneficial to the growth and repr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/22A61L27/40A61L27/36A61L27/24A61L27/20A61L29/12A61L29/00A61L29/04
Inventor 钟春燕钟宇光
Owner 钟春燕
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