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Industrialized cultivation method of microbial biomass and system for realizing the method

A technology of microbial biomass and cultivation method, which is applied in the industrialized cultivation of microbial biomass and in the field of microbial cultivation, and can solve problems such as unsuitability for large-scale industrial production, low service life, and unsmooth production.

Active Publication Date: 2016-11-23
GUANGDONG MARITECH MARINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that it is feasible to use glass or polymer to make a closed reactor with a volume of 10 to 500 cubic meters, but when making a reactor with a volume of 5,000 to 10,000 cubic meters, the input cost of using glass or polymer as a material will be quite high. , and the technical difficulty is extremely high
Therefore, it is difficult to achieve the goal of industrialization with this reactor
[0008] There are three kinds of culture ponds provided by Chinese patent (CN1718722A): the first kind is common culture ponds, and the disadvantage is that it needs to be covered with a greenhouse for sealing, and measures such as insulation layer need to be installed. The investment is high, and it is not suitable for use in southern China.
The second and third types are flexible breeding ponds and flexible breeding bags: the disadvantages are that the HDPE film is easily damaged, the service life is short after exposure to the sun, and the seams are easily damaged. If the failure rate is high in industrial production, it will not be able to produce smoothly.
[0009] The Chinese patent (CN100500828C) uses a small sealed reactor for the cultivation of diatoms. The closed microalgae culture is suitable for the production of high-value products, or as the source of algae species in open culture tanks. The process is complex and requires real-time control of nutrition. ingredients, not suitable for large-scale industrial production
However, when considering industrial scale production and increasing the number of culture vessels to 10-40, many new difficulties will arise

Method used

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  • Industrialized cultivation method of microbial biomass and system for realizing the method
  • Industrialized cultivation method of microbial biomass and system for realizing the method
  • Industrialized cultivation method of microbial biomass and system for realizing the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0173] This example is an example of industrialized cultivation.

[0174] The production process with a single culture tank as a unit includes: culture tank preparation; stirring and aeration system layout; primary nutrient dissolution; pipeline transportation; primary cultivation; secondary nutrient feeding (including secondary nutrient dissolution and pipeline transportation); carbon source mixing Stirring; mixing of materials; secondary cultivation; shutdown to harvest.

[0175] Prepare the culture tank, determine the type of culture tank, clean and fill.

[0176] Culture tank type:

[0177] Cultivation pool area: 2600-5000 square meters

[0178] Depth of cultivation pool: 1.5-2.0 meters

[0179] Cultivation water volume: 4000-10000 cubic meters

[0180] Requirements for the culture tank: the bottom and wall of the tank are hardened with cement, and the bottom and wall of the culture tank are covered with HDPE film.

[0181] cleaning:

[0182] Cleaning requirements: n...

Embodiment 2

[0269] This example is another example of industrialized cultivation.

[0270] The production process based on a single culture tank includes: culture tank preparation; stirring and aeration system layout; primary nutrient dissolution; pipeline transportation; primary cultivation; secondary nutrient feeding (including secondary nutrient dissolution and pipeline transportation); carbon source mixing Stirring; mixing of materials; secondary cultivation; shutdown to harvest.

[0271] Culture pool preparation, determine the type of culture pool, clean and fill:

[0272] Culture pool type:

[0273] Cultivation pool area: 6000 square meters

[0274] Depth of cultivation pool: 2.0 meters

[0275] Cultivation water volume: 12000 cubic meters

[0276] Requirements for the culture tank: the bottom and wall of the tank are hardened with cement, and the bottom and wall of the culture tank are covered with HDPE film.

[0277] cleaning:

[0278] Cleaning requirements: no chemical poll...

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Abstract

The invention discloses an industrial culturing method of microbial biomass. The industrial culturing method comprises following steps: (1) raw materials are dissolved; (2) the dissolved nutriments or carbon sources are delivered into culturing ponds via pipes and raw material transfer pumps; (3) microbial biomass is cultured in the culturing ponds; (4) stirring oxygen aeration is carried out in the whole culturing process; and (5) the system is shut down after a culturing period for collecting. According to the industrial culturing method, large-scale open culturing ponds are adopted, large-scale industrialized microbial biomass production with high efficiency is realized, production efficiency is increased, production cost is reduced, and technical problems of large-scale industrialized production of microbial biomass are solved.

Description

technical field [0001] The invention relates to the field of microbial cultivation, in particular to the technical field of industrialized cultivation of microbial biomass. Background technique [0002] In the Chinese invention patent with the publication number CN102016001A, a method for cultivating microbial biomass is provided. The microbial biomass is a microbial mixed group of microalgae and bacteria, and the mixed group is microalgae, diatoms, yeast, fungi, Flocs of protists, microplankton and bacteria. [0003] Microalgae are used in aquaculture as feed for molluscs, crustaceans and certain fish, and for zooplankton used in the aquaculture food chain. Microalgae play a key role in shrimp farming. [0004] Microalgae-containing microbial biomass flocs can be used as feed additives for crustaceans after drying, but the main difficulty is that it is difficult to obtain products with stable yields and low prices. [0005] The production process of microorganisms such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12M1/00C12M1/06C12M1/02
Inventor 许建刚谢全森王停军宋建华冯卫东黄俊翔王春玉
Owner GUANGDONG MARITECH MARINE BIOTECH
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