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A preparation method of bone-like ceramic composite material loaded with bioactive factors

A technology of ceramic composite materials and biologically active factors, which is applied in the fields of medical preparations containing active ingredients, pharmaceutical formulas, and pharmaceutical sciences. It can solve the problems of poor biological activity, refractory degradation, and poor bone powder strength, and achieve improved hydrophilicity. , Promoting proliferation and differentiation, uniform pores

Active Publication Date: 2015-08-05
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention will aim at the disadvantages of the existing bone powder such as poor strength, difficulty in degradation, poor biological activity, etc., select modified calcined bone powder or particles as the matrix, carry out cross-linking with carboxymethyl chitosan solution, add bone morphogenetic protein BMP- 2. Made into a new type of bone repair material with high strength, easy to degrade and osteoinductive properties

Method used

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  • A preparation method of bone-like ceramic composite material loaded with bioactive factors
  • A preparation method of bone-like ceramic composite material loaded with bioactive factors
  • A preparation method of bone-like ceramic composite material loaded with bioactive factors

Examples

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Embodiment 1

[0034] Step 1, prepare modified calcined bone material; select the joint head of newborn calf as the bone material, fully wash with deionized water and basically remove collagen by physical or biochemical methods; then use diammonium hydrogen phosphate to balance the Modification of the bone matrix (bone salt) by formula matching, so that the Ca / P atomic ratio of the bone matrix is ​​reduced to 1.66; then the modified bone material is calcined at a temperature of 1000°C to completely remove the immune antigenicity of the bone material , to obtain modified calcined bone material.

[0035]By the XRD pattern (X-ray diffraction pattern, Figure 5 ) It can be seen that the crystal phase of the bone matrix changes from HA to a dual-phase structure of HA / β-TCP (Hydroxyapatite / β-tricalcium phosphate, hydroxyapatite: β-tricalcium phosphate), and the bone matrix crystal phase hydroxyapatite: β- Tricalcium phosphate=1:3. SEM observation shows that the calcined bone meal contains both 3...

Embodiment 2

[0044] Step 1, prepare modified calcined bone material; select the joint head of newborn calf as the bone material, fully wash with deionized water and basically remove collagen by physical or biochemical methods; then use ammonium dihydrogen phosphate to balance the bone material according to the chemical reaction Modification of the bone matrix (bone salt) by formula matching, so that the Ca / P atomic ratio of the bone matrix is ​​reduced to 1.55; then the modified bone material is calcined at a temperature of 1000°C to completely remove the immune antigenicity of the bone material , to obtain modified calcined bone material.

[0045] Step 2, select the carboxymethyl chitosan of molecular weight at 10000 for use, prepare the carboxymethyl chitosan aqueous solution of 10mg / ml with triple distilled water, and adjust its pH with acetic acid to be 6.5;

[0046] Step 3, get carboxymethyl chitosan aqueous solution after pH adjustment, modified calcined bone material, bone morphogen...

Embodiment 3

[0051] Step 1, prepare modified calcined bone material; select newborn calf joint head as bone raw material, fully wash with deionized water and basically remove collagen by physical or biochemical methods; then use phosphoric acid to mix according to the chemical reaction equilibrium equation Modify the bone matrix (bone salt) to reduce the Ca / P atomic ratio of the bone matrix to 1.5; then calcinate the modified bone material at a temperature of 1000°C to completely remove the immunoantigenicity of the bone material and obtain the improved Calcined bone material.

[0052] Step 2, select the carboxymethyl chitosan of molecular weight at 40000 for use, prepare the carboxymethyl chitosan aqueous solution of 30mg / ml with triple distilled water, and adjust its pH with acetic acid to be 6;

[0053] Step 3, get carboxymethyl chitosan aqueous solution after pH adjustment, modified calcined bone material, bone morphogenetic protein and mix, make carboxymethyl chitosan:modified calcine...

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Abstract

The invention relates to a preparation method of a bioactive factor loaded bone-like ceramic composite material. The preparation method comprises the following steps: 1. preparing a modified calcined bone material; 2. preparing a carboxymethyl chitosan solution which is 10-30mg / ml through tri-distilled water, and regulating pH to a range of 5.8-6.5; 3. uniformly mixing the carboxymethyl chitosan solution with pH regulated, the modified calcined bone material and bone morphogenetic protein, and molding; and drying at 50-60 DEG C to obtain the bioactive factor loaded bone-like ceramic composite material. A degradation cycle of the prepared bioactive factor loaded bone-like ceramic composite material disclosed by the invention is consistent with bone formation and bone remodeling processes, and the composite material, due to a degradation property thereof, is suitable to serve as an artificial bone substitute material. The material, co-cultivated with two tissue engineering seed cell mesenchymal stem cells, is free from cytotoxicity, and is capable of promoting cell proliferation and differentiation.

Description

technical field [0001] The invention belongs to the field of bioactive composite ceramics, in particular to a method for preparing a bioactive factor BMP-2-like bone ceramic composite material. Background technique [0002] Although there are many methods for bone defect repair and reconstruction, the repair and reconstruction after bone defects, especially the repair and reconstruction after large bone defects, has always been a difficult problem for orthopedics. Seeking ideal substitute materials for bone repair has always been a key issue to be solved in orthopedics and related disciplines. [0003] Years of research results have shown that among the members of the apatite ceramic family, the crystal structure of nano-hydroxyapatite (HA) is basically the same as the structure of mineral particles in bone tissue, and calcium phosphate (TCP) also occupies a certain amount in bone tissue. The ratio, its degradation rate is much higher than that of HA, and it is easy to degr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/46A61L27/12A61L27/20A61L27/54A61L27/56
Inventor 唐志辉李箐廖运茂
Owner PEKING UNIV SCHOOL OF STOMATOLOGY