Preparation method of biological functional titanium material for maintaining dynamic balance of bones
A technology of biological functionalization and bone dynamic balance, applied in the fields of medical science, surgery, coating, etc., can solve the problem of poor drug controlled release performance on titanium surface, low drug loading rate on substrate surface, drug or growth factor inactivation, etc. problem, to achieve the effect of good cell phase and osteoinductive performance, low cost and strong versatility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Example 1: Preparation of biofunctionalized titanium material for maintaining bone homeostasis
[0046]a. Preparation of mesoporous silicon: 0.28g of sodium hydroxide and 1g of cetyltrimethylammonium bromide were uniformly dispersed in distilled water (480mL), vigorously stirred and heated to 80°C. Then, 5 g of tetraethyl orthosilicate was added dropwise, and vigorously stirred for 2 hours until the mixed solution became a white suspension, that is, tetraethyl orthosilicate was hydrolyzed to obtain mesoporous silicon nanomaterials. Mesoporous silicon nanomaterials (CTABMSNs) with surfactants were uniformly dispersed in 150 mL of methanol-hydrochloric acid (135 mL: 15 mL) mixture and stirred vigorously. The above solution was refluxed in a water bath at 80°C for 48 hours, and the surfactant (CTAB) was extracted to obtain mesoporous silica nanoparticles (MSNs) with regular pore structure.
[0047] b. Drug loading: Dissolve β-estradiol in 1,4-dioxane at a concentration of...
experiment example 1
[0051] Experimental Example 1: Cytocompatibility of Biofunctionalized Titanium Surface
[0052] This study investigated the cellular activity of osteoblasts on the surface of biofunctionalized titanium materials.
[0053] Cytocompatibility of substrate surfaces was tested with MTT. First, the primary osteoblasts were cultured, and the third-generation osteoblasts were inoculated on the surface of different titanium membranes and TCPs at a seeding density of 1.5×104cells / cm 2 , cultivated at 37°C. After 4 days, discard the supernatant, add 0.9 mL of fresh medium, and then add 0.1 mL of MTT (5 mg / ml). After culturing for 4 hours, the supernatant was discarded, and 0.7 mL of dimethyl sulfoxide (DMSO) was added to dissolve the crystalline formazan. After complete dissolution, place the solution in a centrifuge tube, centrifuge for 10 minutes, take the supernatant and measure its absorbance at 490nm.
[0054] MTT can be reduced to blue-purple crystalline formazan (Formazan) by ...
experiment example 2
[0055] Experimental example 2: Activity of osteoblast alkaline phosphatase on the surface of biofunctionalized titanium material
[0056] The biofunctionalized titanium material prepared in Example 1 was selected to investigate the alkaline phosphatase activity of osteoblasts on the surface of the material. Firstly, the third-generation primary cultured osteoblasts were inoculated on different titanium materials and TCPS surfaces. After 4 days of culture, the cells were lysed with 1% TitonX-100, and the cell lysate was collected. Alkaline phosphatase activity in the lysate was detected with an alkaline phosphatase detection kit. like Image 6 It is shown that the alkaline phosphatase activity of osteoblasts on the surface of biofunctionalized titanium materials is higher than that of pure titanium materials and titanium materials modified with multilayer films (ie LBL+Ti). At the p<0.01 level, there is a significant difference between biofunctionalized titanium (ie LBL+E2-M...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More