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Preparation technology of bacillus licheniformis inoculant capable of resisting strawberry replant

A technology of Bacillus licheniformis and Bacillus licheniformis, which is applied in the directions of chemicals, applications and biocides for biological control, can solve problems such as the destruction of the atmospheric ozone layer, high cost of use, accumulation of toxic substances, etc., to enhance crop resistance. , the effect of increasing yield and reducing morbidity

Active Publication Date: 2014-07-30
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of using the drug is as high as more than 15,000 yuan per hectare, and the drug effect is limited to that year. In addition, methyl bromide is a highly toxic gas that seriously damages the atmospheric ozone layer. In 2005, the United Nations Environmental Protection Organization listed it as a restricted drug
The use of a large number of toxic chemicals further deteriorates the physical and chemical properties of the soil, reduces fertility, and accumulates toxic substances, seriously affecting the quality and yield of strawberries.

Method used

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  • Preparation technology of bacillus licheniformis inoculant capable of resisting strawberry replant
  • Preparation technology of bacillus licheniformis inoculant capable of resisting strawberry replant
  • Preparation technology of bacillus licheniformis inoculant capable of resisting strawberry replant

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The production process of Bacillus licheniformis inoculant for preventing strawberry cropping is to inoculate Bacillus licheniformis ACCC01957 in a fermentation medium containing carbon sources, nitrogen sources, inorganic salts and trace elements for aeration and fermentation to produce fermentation broth. The fermentation broth is flocculated, Add protective agent, carrier adsorption, spray drying and spray drying to make bacterial powder; the fermentation medium is composed of the following mass percentage components:

[0049] Soybean meal 2.0%; corn syrup dry powder 0.5%; starch 0.5%; glucose 0.4%; MnSO with a concentration of 30.8mg / L 4 Solution 0.1%; NaCl0.05%; defoamer 0.05‰; pH before elimination=7.6±0.1 pH value after elimination 7.0±0.1;

[0050] The process conditions of aerated fermentation are as follows:

[0051] Stirring speed is 150rpm, 0-4 hours: ventilation volume is 1:0.9vvm, temperature is 32℃-36℃; 4-8 hours: ventilation volume is 1:1.0vvm, temperature is 3...

Embodiment 2

[0063] The production process of Bacillus licheniformis inoculant for preventing strawberry cropping is to inoculate Bacillus licheniformis ACCC01957 in a fermentation medium containing carbon sources, nitrogen sources, inorganic salts and trace elements for aeration and fermentation to produce fermentation broth. The fermentation broth is flocculated, Add protective agent, carrier adsorption, spray drying and spray drying to make bacterial powder; the fermentation medium is composed of the following mass percentage components:

[0064] Soybean meal 3.0%; corn syrup dry powder 1.5%; starch 1.5%; glucose 0.6%; MnSO with a concentration of 30.8mg / L 4 Solution 0.3%; NaCl0.15%; defoamer 0.15‰; pH before elimination=7.6±0.1 pH after elimination=7.0±0.1;

[0065] The process conditions of aerated fermentation are as follows:

[0066] Stirring speed is 250rpm, 0-4 hours: ventilation rate is 1:1.1vvm, temperature is 32℃-36℃; 4-8 hours: ventilation rate is 1:1.2vvm, temperature is 33℃-37℃; af...

Embodiment 3

[0078] The production process of Bacillus licheniformis inoculum for preventing strawberry cropping is to inoculate Bacillus licheniformis ACCC01957 on a fermentation medium containing carbon sources, nitrogen sources, inorganic salts and trace elements to produce a fermentation broth. The fermentation broth is flocculated and added. Protective agent, carrier adsorption, spray drying and spray drying to make bacterial powder; the fermentation medium is composed of the following mass percentage components:

[0079] Soybean meal 2.5%; corn syrup dry powder 0.8%; starch 1%; glucose 0.5%; MnSO with a concentration of 30.8mg / L 4 Solution 0.2%; NaCl 0.1%; Antifoaming agent 0.1‰; pH before elimination=7.6±0.1 pH after elimination=7.0±0.1;

[0080] The process conditions of aerated fermentation are as follows:

[0081] Stirring speed is 150-250rpm, 0-4 hours: ventilation is 1:1vvm, temperature is 32℃-36℃; 4-8 hours: ventilation is 1:1.1vvm, temperature is 33℃-37℃; 8 hours After: ventilation...

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Abstract

The invention belongs to preparation of a microbial inoculant, and in particular relates to a preparation technology of a bacillus licheniformis inoculant capable of resisting strawberry replant. The technology comprises the steps of inoculating bacillus licheniformis ACCC01957 into a fermentation medium containing a carbon source and a nitrogen source, and carrying out aerated fermentation to prepare fermentation liquor; carrying out flocculation on the fermentation liquor, feeding a protective agent, carrying out carrier absorption, and carrying out spray drying to obtain fungus powder. The preparation technology solves the problems that the fertility is reduced, and toxic substances are accumulated, so that the quality and the yield of strawberries are seriously influenced in the prior art; all technical indexes of the bacillus licheniformis inoculant capable of resisting the strawberry replant are in accordance with the requirements of GB20287-2006, and the shelf life of the bacillus licheniformis inoculant is more than 18 months; compared with the strawberries fumigated by chloropicrin in a greenhouse, the strawberries treated by the bacillus licheniformis inoculant have obvious advantages in the aspects of technical indexes such as yield, sugar-acid ratio, soluble solid content, and vitamin C content; the bacillus licheniformis inoculant has the advantages that the incidence of diseases can be well reduced, the resistance of crops is improved, the yield of the strawberries is increased, etc.

Description

Technical field [0001] The invention belongs to the production of microbial inoculants, in particular to the production process of Bacillus licheniformis inoculants for resisting strawberry cropping. Background technique [0002] Strawberry stubble disease has always been an important problem plaguing strawberry production, especially with the continuous expansion of the facility industry, this problem has become more and more prominent. At present, the commonly used method for continuous cultivation of strawberries is soil fumigation with the highly toxic pesticide methyl bromide. However, the use cost of the drug is as high as 15,000 yuan per hectare, and the efficacy is only in the current year. In addition, methyl bromide is a highly toxic gas and severely damages the atmospheric ozone layer. In 2005, the United Nations Environmental Protection Organization has listed it as a restricted drug. . The use of a large number of toxic agents further deteriorates the physical and ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P21/00
Inventor 李宾马清河秦艳梅陈文杰罗同阳高庆华郑翔赵从波王云鹏章淑燕
Owner 河北省微生物研究所有限公司
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