Method for analyzing speciation of arsenic elements in smoke of cigarettes

A technology of cigarette smoke and analysis method, which is applied in the field of detection of harmful components of heavy metals in tobacco, and achieves the effects of high precision, high sensitivity and wide linear range

Inactive Publication Date: 2014-07-30
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the characteristics of low interference, high sensitivity, wide linear range, high precision and easy operation of sample pretreatment, HPLC-ICP-MS is widely

Method used

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  • Method for analyzing speciation of arsenic elements in smoke of cigarettes
  • Method for analyzing speciation of arsenic elements in smoke of cigarettes
  • Method for analyzing speciation of arsenic elements in smoke of cigarettes

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Pipette a certain amount of As(V), As(Ⅲ), monomethylarsenic acid (MMA), dimethylarsenic acid (DMA) standard solutions to prepare 0.5, 1.0, 2.0, 5.0, 10.0 and 20.0μg / L mixed series of standard working solutions (both in As) and then determined by HPLC-ICP-MS, linear regression was performed with concentration and CPS counting to obtain a linear equation and correlation coefficient, and the standard working solution was mixed at the lowest concentration for 10 times Determination and calculation of the standard deviations of the four morphological contents, with 3 times the standard deviation as the detection limit. The results showed that the method had good linearity and low detection limit (results are shown in Table 1).

[0018] Table 1. Working standard curves and linear correlation coefficients of 4 kinds of arsenic compounds

[0019] Arsenic form Retention time / min linear equation correlation coefficient R 2 The detection limit As(Ⅲ) 2....

example 2

[0021] Sorting the test cigarettes according to the step (1) in the content of the invention above, and then using a smoking machine with an electrostatic trapping device to smoke 20 test cigarettes according to GB / T 16450-2004, and using an electrostatic trap to capture the particle phase of the smoke Objects were measured 6 times at the same time. With 30mL15mmoL / L (NH 4 ) 2 HPO 4 (pH=6.0) Rinse the particulate matter in the electrostatic collection tube into a 50mL Erlenmeyer flask, shake for 30min, take 5mL of the extract and centrifuge at 10000rpm for 10min, then pipette the supernatant through a 0.45μm micro-membrane, store It was stored in a brown chromatographic bottle for analysis, and then measured by HPLC-ICP-MS to obtain the release of four forms of As element in mainstream cigarette smoke. The results showed that the precision of the method was less than 15% (results are shown in Table 2) .

[0022] Table 2. Parallel determination results (ng / 20 tubes)

[002...

example 3

[0025] Sorting flue-cured cigarettes and mixed cigarettes according to step (1) in the above-mentioned content of the invention, and then using a smoking machine with an electrostatic trapping device to smoke 20 test cigarettes according to GB / T 16450-2004, and using an electrostatic trap to collect Collect flue gas particulate matter and do parallel measurement at the same time. Use 30mL15 mmoL / L (NH 4 ) 2 HPO 4 (pH=6.0) Rinse the particulate matter in the electrostatic collection tube into a 50mL Erlenmeyer flask, shake for 30min, take 5mL of the extract and centrifuge at 10000rpm for 10min, then pipette the supernatant through a 0.45μm micro-membrane, store It was stored in a brown chromatographic bottle for analysis, and then measured by HPLC-ICP-MS to calculate the standard addition recovery rate. The results showed that the method sample had a good standard addition recovery rate (results are shown in Table 3).

[0026] Table 3. Spike recoveries

[0027]

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Abstract

The invention relates to a method for analyzing the speciation of arsenic elements in smoke of cigarettes. The method is characterized by comprising the steps of capturing arsenic in particulate matter of smoke by virtue of a rotary disc smoking machine with an electrostatic capturing device, extracting arsenic compounds of different speciation in the particulate matter by virtue of a diammonium hydrogen phosphate solution, and quantitatively analyzing the release amount of arsenic elements of different speciation by virtue of an HPLC-ICP-MS (High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry) technique so as to realize the quantitative analysis on the speciation of the arsenic elements in main stream smoke of the cigarettes. The method has the advantages that a sample pretreatment process is simple, convenient and rapid, the consumption amount of an extraction reagent is little, a separating effect of the speciation of As elements is good, the analytical interference is less, the sensitivity is high, the linearity range is wide, the precision is high, and a technical support is provided for accurately estimating the security risk of the As elements in the main stream smoke of the cigarettes.

Description

technical field [0001] The invention relates to a technology for detecting harmful components of heavy metals in tobacco, in particular to a HPLC-ICP-MS combined method for determining the form of arsenic in cigarette smoke. The method can be used to quantitatively analyze the released amount of arsenic in different forms. Background technique [0002] Heavy metals are an important class of harmful components in mainstream cigarette smoke. Because the hazards of heavy metal elements are closely related to their existing forms, the toxicity of different forms varies greatly. For example, As element has many chemical forms, and the common ones are As(V), As (Ⅲ), monomethyl arsenic acid (MMA), dimethyl arsenic acid (DMA), etc. Among them, trivalent arsenic is more toxic than pentavalent arsenic, and inorganic arsenic is more toxic than organic arsenic. Therefore, it is of great significance to study the chemical form of arsenic in mainstream cigarette smoke to correctly underst...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08
Inventor 王洪波郭军伟胡斌谢复炜彭斌王昇赵乐刘绍锋刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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