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Rhodococcuscercidiphylli and screening method and application thereof

A technology of Rhodococcus and Rhodococcus, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effects of low operation and maintenance costs, simple operation, and simple methods

Inactive Publication Date: 2014-08-06
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no effective degrading strains have been screened out in groundwater or drinking water filtration units for the biological treatment of nitrosamines at home and abroad.

Method used

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  • Rhodococcuscercidiphylli and screening method and application thereof
  • Rhodococcuscercidiphylli and screening method and application thereof
  • Rhodococcuscercidiphylli and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and purification of strains

[0027] 1. Preparation of culture medium

[0028] (1) Mineral salt medium

[0029] K 2 HPO 4 ·3H 2 O: 4.25.00g, NaH 2 PO 4 ·H 2 O: 1.00g, MgSO 4 ·7H 2 O: 0.20g, FeSO 4 ·7H 2 O: 0.012g, MnSO 4 ·H 2 O: 0.003g, ZnSO 4 ·7H 2 O: 0.003g, CoSO 4 ·7H 2 O: 0.001g, NH 4 Cl 2.0g, 1000mL ultrapure water, pH=7.3, and the mineral salt culture medium was sterilized at 121°C for 20 minutes.

[0030] (2) Solid enrichment medium

[0031] Tryptonye Soya Broth (Oxoid LTD, England) 30g, agar 20g, ultrapure water 1000mL, and the solid enrichment medium is sterilized at 121°C for 20 minutes before use.

[0032] 2. Domestication, enrichment and cultivation

[0033] Microbial domestication and enrichment culture is carried out in the biological activated carbon mobile domestication treatment device. The water in the domestication treatment device is taken from the effluent of the biological activated carbon filter of the drinking water treatment plant, and...

Embodiment 2

[0037] Example 2 Study on Microbiological Characteristics of Rhodococcus L

[0038] 1. Observation of colony morphology

[0039] The isolated and purified Rhodococcus lanceolata strains were streaked and inoculated in a solid enrichment medium, cultured at 25°C until colonies grew, and the morphology of the colonies was observed. The results showed that the colony characteristics of the strains were as follows: The diameter of the colony cultured on the enriched medium plate for 3 days is about 1-2mm, the colony is round, convex, opaque, the center is reddish yellow, and the edges are neat, such as figure 1 Shown.

[0040] 2. Cell morphology observation

[0041] Pick the bacterial colonies cultured on the solid enrichment medium plate for 3 days, fix with glutaraldehyde, rinse with phosphate buffer (pH 7.3), gradient ethanol dehydration, isoamyl acetate treatment and carbon dioxide critical point drying, After spraying gold and placing the sample in the observation room, perform e...

Embodiment 3

[0045] Example 3 Application of Rhodococcus sylvestris

[0046] 1. Preparation of wet cells of Rhodococcus monospora strains

[0047] Use an inoculation loop to take the fresh slant strain of Rhodococcus sylvestris, streak it and inoculate it on the solid enriched medium. After culturing for 3 days at a temperature of 25°C, a medium plate with obvious colonies can be obtained to obtain fresh serrata Rhodococcus arborescens wet cells.

[0048] 2. Test for removing 5 kinds of nitrosamine substances

[0049] Pick wet cells of Rhodococcus sylvestris and inoculate them in a mineral salt medium, and culture them at 25°C under dark conditions. Among them, 0.6g wet cells are inoculated in 100 mL of mineral salt medium; mineral salt culture The mass concentrations of the 5 nitrosamines in the base are 200ng / L respectively. The detection method is based on the previous study and is determined by ultra-high liquid phase tandem mass spectrometry (UPLC / MS / MS) to determine the medium before inoc...

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Abstract

The invention discloses a strain of rhodococcuscercidiphylli and a screening method and application thereof. The technical scheme provided by the invention is that the rhodococcuscercidiphylli is preserved in general microbe center of China Microbiological Culture Collection Preservation Committee with the preservation number of CGMCCNo. 9067. The invention further discloses the screening method of the rhodococcuscercidiphylli and the application of the rhodococcuscercidiphylli in degrading nitrosamines. The screening method of the rhodococcuscercidiphylli, provided by the invention, is simple and fast in operation, and can be applied to drinking water contaminated by nitrosamines for degrading and removing nitrosamines, performing biological treatment on the drinking water contaminated by nitrosamine substances, and reducing risk level.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of nitrosamines in drinking water, and in particular relates to a strain of Rhodococcus spp. and its screening method and application. Background technique [0002] Drinking water disinfection not only ensures biological safety, but also produces a series of disinfection by-products that are harmful to human body. The disinfection by-products of nitrosamines (Nitrosamines) produced in this process have carcinogenic, teratogenic and mutagenic effects, and their toxicity is much higher than that of common halogenated disinfection by-products (trihalomethanes and halogenated Acetic acid, etc.), so it has attracted widespread attention at home and abroad, and has become a hot issue in the field of environmental and health research. According to the United States Environmental Protection Agency (USEPA) pollutant risk information database (Integrated Risk Information System, IRIS)) and st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 王万峰郭彦玲朱春友黄耀樊静张国庆宋琳琳潘峰
Owner HENAN NORMAL UNIV
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