Preparation method of planarian chromosome specimen

A chromosome and planarian technology, applied in the field of cytogenetics, can solve problems such as unfavorable research and analysis, difficult sampling and processing, unstable results, etc., and achieve the effect of good cell dispersion, clear background and good effect
CN103983497BInactive Publication Date: 2016-04-20ZHOUKOU NORMAL UNIV
3 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHOUKOU NORMAL UNIV
Publication Date
2016-04-20
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
Patent Text Reader

Abstract

The invention relates to a method for preparing a planarian chromosome sample. The preparation method of the planarian chromosome specimen of the present invention comprises the following steps: material collection, pretreatment, regeneration tissue culture, tissue crushing, centrifugal suspension, colchicine blocking, KC1 hypotonic treatment, centrifugal suspension dripping, dyeing and drying and sealing piece. The preparation method of the invention fully fixes the regenerated tissue and the metaphase cells, has a clear chromosome preparation background, well-dispersed cells, and many metaphase division phases, which is beneficial to karyotype analysis.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of cytogenetics, and in particular relates to a method for preparing a planarian chromosome sample. Background technique

[0002] Planarians are widely distributed in my country. In the history of animal evolution, planarians are the first group with bilateral symmetry and three germ layers. Their evolutionary status is very important, and they are good materials for the research of developmental biology, cytogenetics and regenerative biology. Chromosome preparation of planarian is the basis of its genetics research, as well as the basis of cell and molecular biology research.

[0003] The existing mature methods for preparing animal chromosome specimens are mostly suitable for higher vertebrates and humans, and can be prepared after processing by collecting bone marrow or blood; while planarians only have interstitial fluid, no spinal cord and blood, so sampling and processing are difficult. Li Guangpeng (Journal of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More