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Single-stranded DNA aptamer modified SiO2/Fe3O4 magnetic microsphere preparation method

A technology of nucleic acid aptamer and ferroferric oxide, which is applied in the field of chemical analysis and testing instruments, can solve the problems of lack of recognition ability and achieve the effects of strong magnetism, large specific surface area, and low immobilization rate of physical adsorption

Active Publication Date: 2014-08-20
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

For example, the Penner group screened out a DNA aptamer that can specifically recognize ochratoxin A, and constructed a DNA aptamer affinity column for the detection of ochratoxin A in wheat samples. Aspergillus A has high selectivity, but has no ability to recognize ochratoxin A structural analogs acetone coumarin and N-acetylphenylalanine (Cruz-Aguado J A, Penner G. Determination of ochratoxin A with a DNA aptamer[J].Journal of Agricultural and Food Chemistry,2008,56(22):10456?10461)

Method used

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  • Single-stranded DNA aptamer modified SiO2/Fe3O4 magnetic microsphere preparation method
  • Single-stranded DNA aptamer modified SiO2/Fe3O4 magnetic microsphere preparation method
  • Single-stranded DNA aptamer modified SiO2/Fe3O4 magnetic microsphere preparation method

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Embodiment Construction

[0025] This embodiment takes the adenosine target molecule as an example to describe the present invention in detail, but this does not limit the protection scope of the present invention.

[0026] like figure 1 Shown, single-stranded DNA aptamer-SiO 2 / Fe 3 o 4 The preparation method of magnetic microspheres is as follows:

[0027] (1) Accurately weigh 14.04g FeCl 3 ·6H 2 O and 7.19g FeSO 4 ·7H 2 O, dissolved in 60mL of boiled and cooled deionized water, poured into a 500mL three-necked flask, washed the beaker with 90mL of boiled and cooled deionized water and transferred all to the three-necked flask, mechanically stirred, and nitrogen deoxygenated for 10min. Transfer the three-neck flask to a 90°C water bath, add 40 mL of 25% ammonia water dropwise with a separatory funnel, then drop 5 mL of oleic acid into the flask at a constant rate of 0.5 mL / min, and stir at constant temperature for 1 h. The obtained magnetic fluid was placed on a magnet for sedimentation for 2...

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Abstract

The present invention discloses a single-stranded DNA aptamer modified SiO2 / Fe3O4 magnetic microsphere preparation method, which comprises: (1) adopting a chemical co-precipitation method to obtain Fe3O4 magnetic nanoparticles; (2) adopting a sol-gel method to obtain SiO2 / Fe3O4 magnetic microspheres; (3) obtaining -NH2 modified SiO2 / Fe3O4 magnetic microspheres; (4) dispersing the -NH2 modified SiO2 / Fe3O4 magnetic microspheres in water to obtain -COOH modified SiO2 / Fe3O4 magnetic microspheres; and (5) dispersing the -COOH modified SiO2 / Fe3O4 magnetic microspheres in a Tris-HCl buffer solution to obtain the aptamer modified SiO2 / Fe3O4 magnetic microspheres.

Description

technical field [0001] The invention relates to the field of chemical analysis testing instruments, in particular to a single-stranded DNA nucleic acid aptamer modified SiO 2 / Fe 3 o 4 Preparation method of magnetic microspheres. Background technique [0002] The samples involved in modern analytical chemistry exist in the form of gas, liquid, and solid phases with complex compositions, and they often interfere with each other during measurement. At the same time, the low concentration and poor stability of analytes also bring problems a series of difficulties. In particular, environmental and biological samples require sample pretreatment before further determination. In a complete sample analysis process, sample pretreatment takes the longest time, accounting for about 2 / 3 of the entire analysis time. Therefore, the exploration of new technologies and methods for sample pretreatment has become one of the important topics and development directions in the field of mode...

Claims

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Application Information

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IPC IPC(8): B01J13/02B01J20/22B01J20/28B01J20/30
Inventor 胡小刚柳晓飞伍莉莉郝丽贤
Owner SOUTH CHINA NORMAL UNIVERSITY
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