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Effective humulus scandens part with antibacterial activity and application thereof

An effective part, the technology of Humulus japonicus, applied in the field of preparation of antibacterial drugs and antibacterial activity, can solve the problem of no antibacterial activity of clinical drug-resistant bacteria, and achieve the effects of potential economic and ecological benefits, strong antibacterial activity, and rich sources of medicinal materials

Inactive Publication Date: 2014-08-27
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Throughout the reports at home and abroad, the research on the extract of Humulus japonicus and its pharmacological activities are mostly focused on the reports on the anti-inflammatory, analgesic, anti-tuberculosis, and anti-oxidation aspects of the extract of Humulus japonicus. Growth studies, in particular no reports of antimicrobial activity against clinically resistant bacteria

Method used

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  • Effective humulus scandens part with antibacterial activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Extraction and preliminary treatment: take the whole herb of Humulus chinensis, adopt about 8 times the amount of 5%-100% alcohol (any one or a mixture of several in methanol, ethanol, n-butanol), and heat it at 70°C Under reflux extraction for 2 hours, repeated twice to obtain the Humulus japonicus extract; take the Humulus japonicus extract, recover and concentrate under reduced pressure, add appropriate ethanol to make the ethanol concentration reach 50%-90%, let stand for precipitation, filter to obtain the filtrate, and recover under reduced pressure to The filtrate has no alcohol smell, and the filtrate is obtained; or the Humulus japonicus extract is extracted with petroleum ether to remove the fat-soluble components, and the filtrate is obtained.

[0019] (2) Crude and refined: the filtrate in step (1) is adsorbed and eluted with a macroporous resin, and the eluent is eluted with 0-30% dilute alcohol (methanol or ethanol) solution to remove impurities, and 40...

Embodiment 2

[0021] (1) Extraction and preliminary treatment: take the whole herb of Humulus chinensis, adopt about 8 times the amount of 5%-100% alcohol (any one or a mixture of several in methanol, ethanol, n-butanol), and heat it at 70°C Under reflux extraction for 2 hours, repeated twice to obtain the Humulus japonicus extract; take the Humulus japonicus extract, recover and concentrate under reduced pressure, add appropriate ethanol to make the ethanol concentration reach 50%-90%, let stand for precipitation, filter to obtain the filtrate, and recover under reduced pressure to The filtrate has no alcohol smell, and the filtrate is obtained, or the extract of Humulus japonicus is taken, extracted with petroleum ether to remove the fat-soluble components, and the filtrate is obtained, and the above obtained filtrate is concentrated and dried to obtain the crude extract.

[0022] (2) Refining: the crude extract obtained in step (1) is subjected to silica gel column chromatography, and the...

Embodiment 3

[0024] The effective part of Humulus japonicus obtained in Example 1 and Example 2 is separated from the monomeric compound, through repeated silica gel column chromatography, MCI column chromatography, Sephadex-LH20 (CHCl 3 -MeOH=1:1, MeOH) column chromatography, ODS reverse-phase column chromatography and recrystallization etc. separation methods, therefrom isolate apigenin, luteolin, vitexin, luteolin-7-O-β-D -Glucoside, cosmoside, mongoside and N-p-coumaryl tyramide and other monomer compounds, in which apigenin, luteolin, vitexin, luteolin-7-O-β-D- Mainly flavonoids such as glucoside and cosmoside.

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Abstract

The invention discloses an effective humulus scandens part, the antibacterial activity of the effective humulus scandens part and the application of the effective humulus scandens part to preparation of antibacterial drugs. The whole plants of humulus scandens are used as the raw material, extraction is performed through water or 5% to 100% alcohol, after the primary treatment, the effective humulus scandens part is obtained through the macroporous resin-polyamide resin combination technology or the silica-gel column chromatography and mainly contains flavonoids compounds, the total flavonoid content ranges from 20 percent to 90 percent, and the effective humulus scandens part mainly contains apigenin, luteolin, luteolin-7-O-beta-D-glucoside, cosmosiin and the like. The effective part has the remarkable antibacterial activity and shows high antibacterial activity to clinically methicillin-resistant staphylococcus aureus, methicillin-resistant staphylococcus epidermidis, enterococcus faecium and enterococcus faecalis. The effective humulus scandens part can be used as the raw material of medicines and made into antibacterial drug tablets and capsules and the like and particularly can be used for preparing drugs for treating diseases of the urinary system such as diarrhea, enterogastritis, pyelonephritis and acute nephritis caused by bacterial infection and foods with the digestion aid, prevention and health care functions.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations, and in particular relates to a flavonoid compound of an effective part of Humulus japonicus, the antibacterial activity of the effective part and its use in the preparation of antibacterial drugs. Background technique [0002] In recent years, due to the abuse of antibiotics and the evolution of bacteria, the problem of drug resistance in clinical bacterial infections has become more and more serious. As a major country in the production and use of antibiotics, drug-resistant infectious diseases have become the main factor in the death of clinical infectious diseases in my country. Therefore, the development of low toxicity and effective drug resistance inhibitors has wide application value and good prospects. Studies have found that certain Chinese herbal medicines have broad-spectrum antibacterial effects and can improve the body's immunity. Therefore, the problem of dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/60A23L1/29A61P31/04A61P1/14A23L33/00
CPCY02A50/30
Inventor 蒋建勤徐保磊阳文波
Owner CHINA PHARM UNIV
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