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Method for extracting ganoderic acid A from ganoderma lucidum

A technology of ganoderma acid and ganoderma lucidum, applied in the field of extraction and separation of natural products, can solve the problems of large environmental pollution and low yield, and achieve the effect of high purity, high yield and convenient operation

Inactive Publication Date: 2014-09-10
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention provides a kind of method for extracting ganoderma acid A from ganoderma in order to solve the technical problems such as large environmental pollution and low yield due to the use of chloroform as the extraction solvent in the extraction process of the above-mentioned ganoderma acid A. The method is environmentally friendly, and the yield and purity of the finally obtained ganoderma acid A are relatively high

Method used

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  • Method for extracting ganoderic acid A from ganoderma lucidum
  • Method for extracting ganoderic acid A from ganoderma lucidum
  • Method for extracting ganoderic acid A from ganoderma lucidum

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Embodiment 1

[0030] A method for extracting ganoderma acid A from ganoderma, specifically comprising the following steps:

[0031] (1), get 1Kg dried Ganoderma lucidum and pulverize, the obtained Ganoderma lucidum powder is leached 3 times with 5L mass percentage concentration of 80% ethanol aqueous solution, combine the leaching solution of 3 times gained, control temperature during each leaching process is 60℃, the time is 5h;

[0032] Take the leaching solution obtained by combining the above three times, and use Agilent1260DAD for detection, chromatographic column: Ultimate LP-C18 (5μm, 4.6mm x 250mm ), flow rate: 1.0mL / min, chromatographic column temperature: 25°C, detection wavelength: 256nm, Injection volume: 20 μL, mobile phase acetonitrile: 0.5% formic acid gradient elution, the chromatogram obtained is as follows figure 1 As shown, GA in the figure represents the absorption peak of ganoderma acid A in ganoderma medicinal materials, from figure 1 It can be seen that the compound...

Embodiment 2

[0048] A method for extracting ganoderma acid A from ganoderma, specifically comprising the following steps:

[0049] (1), take 10kg of dried Ganoderma lucidum and pulverize it, and extract the obtained Ganoderma lucidum powder with 50L of 80% ethanol aqueous solution for 3 times, combine the obtained extracts for 3 times, and control the temperature during each extraction process. 60℃, the time is 5h;

[0050] The amount of Ganoderma lucidum powder and 80% ethanol aqueous solution used for above-mentioned leaching is calculated according to the ratio of 1Kg: 5L of Ganoderma lucidum powder: 80% ethanol aqueous solution with a mass percentage concentration;

[0051] (2), control the temperature of the extract solution obtained in step (1) to be 60°C, and the pressure is -0.1MPa to concentrate under reduced pressure to obtain Ganoderma lucidum acid extract;

[0052] (3), add water 5 times the mass of Ganoderma lucidum acid extract to the Ganoderma lucidum acid extract obtained ...

Embodiment 3

[0062] A method for extracting ganoderma acid A from ganoderma, specifically comprising the following steps:

[0063] (1), get 100kg dry Ganoderma lucidum and pulverize, the Ganoderma lucidum powder of gained is divided into 5 times, each time 20kg Ganoderma lucidum powder is leached 3 times with 80% ethanol aqueous solution with 200L mass percent concentration, merges the leaching solution of 3 times gained, During each extraction process, the temperature is controlled at 60°C, and the time is 5 hours each time;

[0064] The amount of Ganoderma lucidum powder used in the above-mentioned extraction and the ethanol aqueous solution with a mass percentage concentration of 80% is calculated according to the ratio of Ganoderma lucidum powder: 80% ethanol aqueous solution with a mass percentage concentration of 1Kg: 10L;

[0065] (2) Control the temperature of the extract solution obtained in step (1) to 60°C and the pressure to -0.1MPa to concentrate under reduced pressure to obta...

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Abstract

The invention provides a method for extracting ganoderic acid A from ganoderma lucidum, which comprises the following steps: leaching ganoderma lucidum powder with a 80 wt% ethanol water solution, concentrating the obtained leaching solution under reduced pressure to obtain a ganoderic acid extractum, adding water, uniformly mixing to obtain a mixed solution, adding NaHCO3 which accounts for 5 wt% of the mixed solution, stirring uniformly, filtering to obtain a filtrate, regulating the pH value to 2, extracting with 5 times by volume of chloroform, concentrating the obtained chloroform layer under reduced pressure, purifying the concentrated solution with a silica gel column, eluting the collected ganoderic acid A crude product with a 51 wt% methanol water solution on an LH-20 normal pressure gel column until all the ganoderic acid A crude product is eluted down, concentrating under reduced pressure to a dry state to obtain ganoderic acid A with the purity of higher than 85%, and recrystallizing with methanol to obtain the ganoderic acid A with the purity of higher than 97.5%. The total yield of ganoderic acid A in the ganoderma lucidum drug is 35%.

Description

technical field [0001] The invention belongs to the extraction and separation method of natural products, in particular to a method for extracting ganoderma acid A from ganoderma lucidum. Background technique [0002] Ganoderma lucidum is a medicinal fungus belonging to the Ganoderma lucidum family. It was first recorded in the "Shen Nong's Herbal Classic" in the Han Dynasty and listed as the top grade. There are more than 80 kinds of Ganoderma lucidum in the genus Ganoderma. chemical composition. The main active ingredients of Ganoderma lucidum are polysaccharides and peptidoglycans, alkaloids and nucleotides, amino acids and proteins, three types of amino acids, and trace elements. Triterpenoids in Ganoderma lucidum have hepatoprotective effect, anti-tumor effect, anti-HIV1 and HIV2 protease activity, inhibition of histamine release, inhibition of angiotensin-converting enzyme (ACE), inhibition of cholesterol synthesis, analgesic effect, and influence on platelet aggregat...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 李成飞张婉萍冯宝军
Owner SHANGHAI INST OF TECH