Beta-lactoglobulin peptides for treating cow's milk protein allergy

A technology of milk protein and lactoglobulin, applied in the direction of protein-containing food ingredients, peptides, applications, etc., can solve the problems of infants who are not suitable for milk allergies

Inactive Publication Date: 2014-09-10
NUTRICIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] However, prior art hypoallergenic compositions generally provide their effect only by avoiding the presence of potential allergens, thus only providing secondary allergy prevention and / or they are only suitable for infants at risk of developing allergies. Not suitable for infants with established cow's milk allergy as it may cause allergic reactions in such infants

Method used

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  • Beta-lactoglobulin peptides for treating cow's milk protein allergy
  • Beta-lactoglobulin peptides for treating cow's milk protein allergy
  • Beta-lactoglobulin peptides for treating cow's milk protein allergy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Peptide selection

[0088] An 18 amino acid long synthetic peptide with a 12 amino acid overlap spanning the β-lactoglobulin B variant and 6 polymerized peptides of the β-lactoglobulin A variant was obtained from JPT Peptide Technologies (Berlin, Germany).

[0089] Peptides were synthesized and pre-screened by analysis of T cell lines. 25 B cells transformed with 18-Epstein Barr virus (EBV) were cultured in RPMI1640-GlutaMAX supplemented with 10% heat-inactivated FBS and 2% Pen / Strep TM -I. Milk-specific T cell lines (TCLs) were generated as previously described by Schade et al. (2000, J Allerg. Clin. Immunol. 106:1155-62). TCL was cultured in Yssel medium containing 2% HS, 2% Pen / Strep, 1% Glut, 50IU / ml IL-2 and 50IU / ml IL-4, and re-stimulated with milk every two weeks to maintain it in medium. For restimulation, autologous EBV-transformed B cells were pre-incubated overnight with 50 μg / ml bovine milk protein mixture. Subsequently, B cells were irradiated and adde...

Embodiment 2

[0100] Treatment of whey protein-allergic mice with β-lactoglobulin peptides results in absence of anaphylaxis

[0101] Three-week-old pathogen-free female C3H / HeOuJ mice (Charles River Laboratories, Maastricht, The Netherlands) were used in this experiment. Mice were fed standard mouse chow without bovine milk protein (AIN-93G soybean, Special Diets Services, Wijk bij Duurstede, Netherlands). They were housed in the animal house of the University of Utrecht and handled according to the guidelines of the Dutch Committee for Animal Experiments.

[0102] Whey protein was obtained from DMV International (Veghel, The Netherlands). Partial whey hydrolyzate (pWH) is produced by enzymatic hydrolysis at the Danone Research Center for Specialty Nutrition under the specific conditions described below. 19.5 kg of demineralized water at 12°C (hereinafter referred to as "demineralized water") was placed in a tank and mixed with 4.1 kg of demineralized whey (Deminal, Friesland Foods Domo)...

Embodiment 3

[0116] Infant formula for infants with cow's milk allergy

[0117] Energy density: 0.6-0.77kcal / ml; lipid: 5.5 to 7g / 100ml ready-to-eat formula milk powder. The protein exists in the form of free amino acids as well as the β-lactoglobulin peptides of the invention. The mixture of peptides as tested in Example 2 was present at approximately 100 μg per g of protein.

[0118] nutritional information

per 100g powder

per 100kcal*

per 100ml**

Energy kJ

2918

413

292

kcal

707

100

70.7

protein g

15.2

2.1

1.5

carbohydrate g

7.6

1.1

0.76

[0119]

[0120]

[0121]

[0122] The composition further comprises vegetable and microbial fats with 0.2 wt% DHA based on total fat.

[0123]

[0124]

[0125]

[0126]

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Abstract

The present invention relates to a nutritional, preferably enteral, composition for use in the treatment of cow's milk allergy in infants allergic to cow's milk containing specific beta-lactoglobulin peptides, which are able to reduce, in particular abolish the acute symptoms of cow's milk protein allergy.

Description

[0001] illustrate technical field [0002] The present invention relates to a nutritional composition, preferably an enteral composition, comprising specific β-lactoglobulin peptides, which is used for the treatment of cow's milk allergy in infants allergic to cow's milk, especially whey protein, and which is capable of alleviating especially Elimination of acute symptoms of cow's milk protein allergy. Background technique [0003] During normal nutrition, dietary protein is presented to the immune system via the gastrointestinal tract without generating an immune response to the ingested nutrient. This non-responsiveness is known as oral immune tolerance or oral tolerance. The induction of oral immune tolerance is especially relevant to infants, who are exposed to dietary protein for the first time after birth and have to adapt to this. Food allergy can occur if the infant's oral immune tolerance is not established. [0004] About 2 to 3% of infants are allergic to cow's...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/305A23L33/00
CPCA61K39/35A23L1/3056A23V2002/00A23L1/3053A23L1/296C07K7/08A23L33/18A23L33/19A23L33/40A23V2250/284A23V2250/54244A23V2250/55A23V2200/304
Inventor 莱昂·马蒂厄·约翰尼斯·科尼波斯约翰·加森劳拉·安托瓦妮特·彼得罗妮拉·玛丽亚·纽勒波洛克
Owner NUTRICIA
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