Sealwort extract as well as preparation method and use thereof

A technology of extract and Polygonatum, applied in the field of plant extract and its preparation, can solve problems such as lowering blood sugar

Inactive Publication Date: 2014-10-01
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous reports found that Polygonatum polysaccharides and Polygonatum saponins both have the effect of lowering blood sugar, and Polygonatum polysaccharides can reduce the expression of AGEs receptor (RAGE) gene, which suggests that Polygonatum can treat diabetes and its complications through a variety of ways, including hypoglycemic and Inhibit the mechanism of AGEs, but Polygonatum and its components have not been reported to inhibit the generation of AGEs

Method used

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  • Sealwort extract as well as preparation method and use thereof
  • Sealwort extract as well as preparation method and use thereof
  • Sealwort extract as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of active ingredients

[0031] Experimental equipment: BRUKER AVⅢ nuclear magnetic resonance spectrometer (400MHz, Bruker, Switzerland), METTLER TOLEDO ten-thousandth balance (Mettler-Toledo Instrument Co., Ltd.), SHZ-D (Ⅲ) circulating water multi-purpose vacuum pump, DLSB- 5 / 25 type low-temperature coolant circulation pump, RE-52A rotary evaporator (Gongyi Yingyu Yuhua Instrument Factory).

[0032] Experimental materials: reversed-phase C18 column chromatography silica gel ODS-A-HG (Japan YMC Company), D101 macroporous adsorption resin (Tianjin Haiguang Chemical Co., Ltd.), used petroleum ether, ethyl acetate, n-butanol, 95% ethanol All are analytically pure. The materials used in this study were purchased from the medicinal materials market in Anguo, Hebei, and were identified as the dried rhizomes of Polygonatum sibiricum Red. by Associate Professor Li Tianxiang from the School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine...

Embodiment 2

[0042] Determination of the activity of inhibiting AGEs production

[0043] Experimental instruments: Flexstation 3 multifunctional microplate reader (MD Company), METTLER TOLEDO one-thousandth balance (Mettler-Toledo Instrument Co., Ltd.).

[0044] Experimental materials: Disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, dipotassium hydrogen phosphate, sodium hydroxide, bovine serum albumin (BSA), and glucose were all purchased from Sigma Company of the United States, and aminoguanidine was purchased from TCI Company of Japan.

[0045] (1) Solution preparation:

[0046] Reactants include 1mol L -1 Glucose aqueous solution and 4mg·mL -1 bovine serum albumin solution, wherein bovine serum albumin with 0.2mol L -1 Phosphate buffer (pH = 7.4) prepared. The positive control drug is 1mg·mL -1 Aminoguanidine aqueous solution. For protein precipitation, trichloroacetic acid aqueous solution with mass (m): volume (v) = 1:1 was used. Dissolve the precipi...

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Abstract

The invention relates to a sealwort extract as well as a preparation method and a use thereof. The sealwort extract is prepared by use of a method comprising the steps of performing reduced-pressure reflux on an extract obtained by performing ethanol hot extraction on sealwort, thereby obtaining a total extract, adding distilled water to the total extract for suspending, extracting by use of isovolumetric petroleum ether, ethyl acetate and n-butyl alcohol orderly, and recovering the solvents under reduced pressure, thereby obtaining an ethyl acetate extract part; the sealwort extract has inhibition effect on AGEs (Advanced Glycation End Products), and thus can be further applied to preparing medicines for treating diabetes and complications thereof and has important clinical application value.

Description

technical field [0001] The present invention relates to a plant extract and its preparation method and application, especially Polygonatum extract, its preparation method and application. Background technique [0002] According to a report in the Journal of the American Medical Association on September 4, 2013, the number of diabetic patients in China exceeds 100 million, which is one of the major diseases in the spectrum of diseases. Diabetic vascular complications are the main cause of amputation, blindness, death and other diseases in diabetic patients, and Advanced Glycosylation End-products (Advanced Glycosylation End-products, AGEs) are one of the important causes of diabetic vascular complications. AGEs are irreversible polymers formed by a series of reactions between proteins and reducing sugars, which can induce vascular endothelial dysfunction, glomerular basement membrane thickening and other lesions, leading to diabetic complications. Therefore, AGEs inhibitors ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969A61P3/10
Inventor 吴红华徐砚通刘东岩汪娟朱彦张鹏董鹏志刘二伟
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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