Method for preparing antibacterial plant tissue culture medium

A plant tissue and culture medium technology, applied in the direction of plant cells, nanotechnology, etc., can solve the problem of difficult dispersion of nanomaterials, and achieve the effect of simple and easy to operate, low cost and uniform particle size

Inactive Publication Date: 2014-10-01
ANKANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage of adding nano silver directly to the medium is tha

Method used

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  • Method for preparing antibacterial plant tissue culture medium
  • Method for preparing antibacterial plant tissue culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0023] At 16°C, measure 20mL with a small graduated cylinder and the concentration is 1.0×10 -3 mol / L silver nitrate aqueous solution in a three-necked flask, add 5mL0.005mol / L ammonia solution, place the three-necked flask on a magnetic stirrer to stir, mix the solution evenly, add 10mL0.015mol / L glucose solution, and then add a few drops 0.01mol / L sodium hydroxide aqueous solution, adjust the pH of the whole solution to 9, react at room temperature for 40min, at this time the color of the solution system does not change. The particle size of nano-silver is irregular spherical, the particle size distribution range is narrow, and the average particle size is 52nm under transmission electron microscope; the antibacterial experiment shows that it has certain antibacterial properties.

Embodiment 2

[0025] At 20°C, measure 20mL with a small graduated cylinder and the concentration is 3.0×10 -3 mol / L silver nitrate aqueous solution in a three-necked flask, add 5mL0.005mol / L ammonia solution, place the three-necked flask on a magnetic stirrer to stir, mix the solution evenly, add 10mL0.015mol / L glucose solution, and then add a few drops 0.01mol / L sodium hydroxide aqueous solution, adjust the pH of the whole solution to 9, and react at room temperature for 1h. The nano-silver particle size observed by transmission electron microscopy is irregular spherical, the particle size distribution range is wider than that of Example 1, and the average particle size is 65nm; when added to MS medium and B5 medium respectively, it has very excellent antibacterial properties.

Embodiment 3

[0027] 1. Preparation of nano-silver sol

[0028] At 15-25°C, take 10-30mL silver nitrate aqueous solution with a small measuring cylinder to prepare a certain concentration in the three-necked bottle, add 2-10mL ammonia solution, stir the three-necked bottle on a magnetic stirrer, and add 5 ~20mLD-glucose aqueous solution, then add a few drops of sodium hydroxide aqueous solution, adjust the pH of the whole solution to 8~12, react at this temperature for 0.5~2h, and the sol system is neutral.

[0029] 2. Nano-silver sol was added to the culture medium

[0030] 1) Dissolve agar in distilled water (agar concentration: 5-7g / L) at 100°C. When the agar is not solidified after cooling, pipette 100mL of the agar solution into a clean beaker and add 5-15mL of the mixture generated in step 1. Silver sol, and add 10-20mL sucrose aqueous solution with a concentration of 20-30g / L to it, mix well and pour into a clean petri dish.

[0031] 2) The other agar solution does not add silver s...

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Abstract

The invention discloses a method for preparing an antibacterial plant tissue culture medium. According to the invention, a green pollution-free and low-cost nano-silver preparation method is adopted, which comprises the following steps of reducing a silver nitrate solution with D-glucose or galactose as a reducing agent in an aqueous solution by virtue of a liquid-phase chemical reduction method at 15-25 DEG C, and adding the obtained nano-silver sol into a culture medium, adding sucrose as a carbon source and an energy material and testing the resistance to bacteria in air of the nano-silver sol. The green pollution-free and low-cost nano-silver preparation method disclosed by the invention is simple and easily operated, the prepared nano-silver sol has the characteristics of uniform and moderate particle size and uniform dispersion. The nano-silver sol disclosed by the invention has good compatibility with the culture medium system. The culture medium in which the nano-silver sol is added has a good antibacterial effect.

Description

technical field [0001] The invention relates to the technical field of tissue culture medium preparation, in particular to a preparation method of an antibacterial plant tissue culture medium. Background technique [0002] Contamination is a ubiquitous problem in tissue culture. In the traditional plant tissue culture process, the bacteria carried or bred by the plant itself are difficult to remove, and will be brought into the culture medium, causing pollution. In addition, in the process of plant reproduction, factors such as unscrupulous sterilization of medium and workbench and irregular operation will lead to pollution, which makes traditional tissue culture plants grow slowly and have a low survival rate, which inhibits the development of plant tissue culture. Wide application and promotion in actual agricultural production. Therefore, if plants can grow under relatively open conditions, it can not only reduce the input of manpower and material resources, but also he...

Claims

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Application Information

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IPC IPC(8): C12N5/04B22F9/24B82Y40/00
Inventor 张芯
Owner ANKANG UNIV
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