Preparation method for fructo-oligo saccharide feed additive

A technology for feed additives and fructooligosaccharides, applied in the field of preparation of fructooligosaccharides feed additives, can solve problems such as inability to produce fructooligosaccharides in large quantities with high efficiency, and achieve the effects of reducing feed costs, simplifying production processes, and enhancing competitiveness

Active Publication Date: 2014-10-29
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned technical solutions are still unable to efficiently produce fructooligosaccharides in large quantities for feed addition.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A strain of Aspergillus niger (Aspergillus niger) BLCY-02 was preserved on July 15, 2014 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, with the preservation number CGMCC No.9449, address: No. 1, Beichen West Road, Chaoyang District, Beijing 3 Institute of Microbiology, Chinese Academy of Sciences.

[0033] The original bacterial strain of Aspergillus niger (Aspergillus niger) BLCY-02 of the present invention is isolated from the soil near the fructooligosaccharide production workshop of Bailong Chuangyuan, Dezhou, Shandong, and the specific separation process is as follows:

[0034] (1) Enrichment culture

[0035] Select the soil near the fructooligosaccharide production workshop in Bailong Chuangyuan, Dezhou, Shandong, remove the topsoil with a small shovel, take about 10g of soil at a distance of 5-15cm from the ground, dilute 10 times with sterile water, and add Cha's medium for enrichment Culture, Cha's medium compo...

Embodiment 2

[0048] A preparation method of fructooligosaccharide feed additive, the steps are as follows:

[0049] (1) Prepare a sucrose solution with a mass concentration of 25%, adjust the pH of the sucrose solution to 5, and prepare a reaction solution at a temperature of 20° C.;

[0050] (2) Inoculate the Aspergillus niger seed liquid in the reaction solution that step (1) makes, inoculum size is 5% of reaction solution volume, under 20 ℃ of conditions, ferment and cultivate 20h, then press the inoculum size of 3% by volume percentage Saccharomyces cerevisiae was added to continue fermentation for 10 hours, and then sterilized to obtain fructooligosaccharide feed additive liquid;

[0051] (3) The fructooligosaccharide feed additive liquid obtained in step (2) is dried by a belt dryer and pulverized by a pulverizer to obtain a fructooligosaccharide feed additive.

[0052] Aspergillus niger seed liquid in described step (2) is prepared as follows:

[0053] 1. Aspergillus niger BLCY-02...

Embodiment 3

[0062] A preparation method of fructooligosaccharide feed additive, the steps are as follows:

[0063] (1) Prepare a sucrose solution with a mass concentration of 60%, adjust the pH of the sucrose solution to 6, and prepare a reaction solution at a temperature of 40° C.;

[0064] (2) Inoculate the Aspergillus niger seed liquid in the reaction liquid that step (1) makes, the inoculum size is 40% of the reaction liquid volume, under 40 ℃ of conditions, ferment and cultivate 25h, then press the 8% inoculum size of percentage by volume Saccharomyces cerevisiae was added to continue fermentation for 15 hours, and then sterilized to obtain fructooligosaccharide feed additive liquid;

[0065] (3) The fructooligosaccharide feed additive liquid obtained in step (2) is dried by a belt dryer and pulverized by a pulverizer to obtain a fructooligosaccharide feed additive.

[0066] Aspergillus niger seed liquid in described step (2) is prepared as follows:

[0067] 1. Aspergillus niger st...

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Abstract

The invention relates to a preparation method for a fructo-oligo saccharide feed additive. The preparation method comprises the following steps of (1) preparing a saccharose solution to prepare reaction liquid; (2) inoculating aspergillus niger strain liquid into the reaction liquid for fermentation cultivation, then adding wine-making yeast for continuous fermentation, and performing sterilization to prepare fructo-oligo saccharide feed additive liquid; and (3) performing drying and smashing on the fructo-oligo saccharide feed adding liquid to obtain the fructo-oligo saccharide feed additive. According to the preparation method, aspergillus niger strains with high fructosyl transferase activity are selected from soil for direct fermentation to produce fructo-oligo saccharide; the activity of fructosyl transferase produced by the strains is improved by over 15-20 percent when compared with the activity of enzyme produced by the conventional aspergillus niger; meanwhile, due to yeast fermentation, the content of the fructo-oligo saccharide in the final product is improved by over 20 percent.

Description

technical field [0001] The invention relates to a preparation method of a fructooligosaccharide feed additive, which belongs to the technical field of functional sugar production. Background technique [0002] With the continuous development of China's aquaculture industry in recent years, the problem of overuse of antibiotics in feed is becoming more and more serious. According to statistics, more than half of the antibiotics produced in China are used to add feed, resulting in excessive antibiotic residues in animal food, drug-resistant bacteria and even "super bacteria" and other problems continue to appear, posing a major threat to human health In view of the various side effects of antibiotics and the impact on food safety, the European Union has banned the addition of antibiotics in animal feed in 2006. From the current point of view, China's ban on the use of antibiotics in feed is also the trend of the times. [0003] Fructose oligosaccharides, also known as fructoo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K10/12A23K20/163A23K40/00
Inventor 李方华邵先豹干昭波窦光朋杨腾腾
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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