Method for preparing glucose and fructose mixture from bagasse

A sugarcane bagasse and mixed liquor technology are applied in the field of sugarcane to achieve the effects of soft taste, low cost and reduced production cost

Active Publication Date: 2016-08-24
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of microorganisms to degrade bagasse to obtain a mixture of glucose and fructose

Method used

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  • Method for preparing glucose and fructose mixture from bagasse
  • Method for preparing glucose and fructose mixture from bagasse
  • Method for preparing glucose and fructose mixture from bagasse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 enzymatic hydrolysis of bagasse to prepare the method for glucose and fructose mixed solution

[0035] 1) Activate the strain Bacillus aryabhattai GZ03 with an activation medium plate (1% peptone, 0.2% yeast extract, 1.5% agar powder, prepared in situ with seawater, sterilized at 121°C for 20 minutes before use), and pick a single The colony was inoculated in the seed medium (1% peptone, 0.2% yeast extract, prepared in situ with seawater, sterilized at 121°C for 20 minutes before use), and cultured at 28°C with shaking at 200r / min for 12h.

[0036] 2) Add the seed solution to the sugar-producing medium (containing 2% of bagasse powder sieved through a 60-mesh sieve, prepared in situ with seawater, and sterilized at 121°C for 20 minutes before use) according to the inoculation ratio of 2%, and continue at 28°C Under the condition, shake culture at 200r / min for 48h to obtain fermentation broth.

[0037] 3) The fermentation broth was centrifuged at 10000×g fo...

Embodiment 2

[0066] Similar to Example 1, the difference is that the culture temperature in steps 1) and 2) is 25°C, the content of yeast extract in the seed medium described in step 1) is 0.5%, and the bagasse powder in step 2) 2%, the culture time is 72h.

Embodiment 3

[0068] Similar to Example 1, the difference is that the culture temperature in steps 1) and 2) is 37°C, the content of yeast extract in the seed medium described in step 1) is 1.5%, and the bagasse powder in step 2) 5%, the culture time is 96h.

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Abstract

The invention relates to sugarcane and provides a method for preparing mixed liquor of glucose and fructose from bagasse. The accession number of the bacterial strain Bacillus aryabhattai GZ03 is CCTCC No: M2013521. The method for preparing mixed liquor of glucose and fructose from bagasse comprises the following steps: 1) activating the bacterial strain Bacillus aryabhattai GZ03 with an activation medium plate, inoculating the bacterial strain in a seed medium and carrying out shake cultivation so as to obtain a seed liquid; 2) inoculating the seed liquid obtained in the step 1) into a sugar-producing medium, continuing shake fermentation cultivation so as to obtain fermentation broth, carrying out centrifugation and then collecting a supernatant; and 3) subjecting the supernatant collected in the step 2) to filtering, wherein the obtained filtrate is the mixed liquor of glucose and fructose. The mixed liquor of glucose and fructose can be used in preparation of food additives and health product additives; the food additives and health product additives may be sweeteners which are especially added into beverages.

Description

technical field [0001] The invention relates to sugarcane, in particular to a method for preparing glucose and fructose mixed liquid from bagasse. Background technique [0002] Sugarcane is a plant rich in sugar and fiber. Bagasse is the main by-product of sugarcane sugar production and is a sustainable biomass resource. Bagasse generally contains 35.4% cellulose, 20.6% hemicellulose, 18.6% lignin, 8.3% crude ash, 3.8% crude protein, 2.8% soluble sugar, 1.5% starch and 3.3% uronic acid. There is a large amount of bagasse remaining in sugar cane after sugar production. According to the current annual output of 11 million tons of sucrose in my country, the national sugar enterprises can have 6-6.5 million tons of bagasse left each year. Rational use of bagasse to reduce production waste and realize the recycling and development of bagasse resources has attracted increasing attention. [0003] In recent years, the main uses of bagasse are: development of feed, cultivation of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/14C12P19/02C12R1/07
Inventor 曾润颖温键易志伟产竹华陈兴麟
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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