Method of preparing chiral phenylalanine by catalysis and asymmetric reduction of marine strain

Abstract

The invention discloses a method of preparing chiral phenylalanine by catalysis and asymmetric reduction of a marine strain, and relates to chiral phenylalanine. Bacillus nanhaiensis DSP-15A2 is preserved in CGMCC (China General Microbiological Culture Collection Center) with a preservation number of CGMCC No. 8969. The method comprises the steps of adding the bacillus nanhaiensis to a 2216L culture medium for culture to obtain a fermentation solution, centrifuging to obtain a cell, resuspending and washing with a buffer solution, preparing a cell solution, adding phenylpyruvic acid and an amino donor to the cell solution as substrates, adding an auxiliary substrate for cyclic regeneration of coenzyme, performing a reaction to obtain a reaction solution containing a product namely L-phenylalanine, centrifuging to remove deposit, adding equivalent methanol to a supernate, shaking and mixing uniformly, centrifuging to remove deposit (protein), diluting the supernate, then measuring, detecting the concentration of phenylpyruvic acid by high performance liquid chromatography, and measuring the concentration of the product namely L-phenylalanine and an excessive value of an antipode by the high performance liquid chromatography.

Description

technical field [0001] The invention relates to chiral phenylalanine (L-phenylalanine), in particular to a method for preparing chiral phenylalanine by catalytic asymmetric reduction of marine strains. Background technique [0002] Chiral amino acids (natural L-amino acids and unnatural D-amino acids) are key intermediates for the synthesis of fine chemicals such as chiral drugs, chiral pesticides and chiral food additives. The preparation of many new drugs (such as protease inhibitors, anti-AIDS drugs, etc.) requires the use of artificially synthesized unnatural chiral amino acids. The synthesis of many chiral amino acids is inseparable from the catalysis of oxidoreductases, so the search for highly selective oxidoreductases has become a hot spot in the research of chiral amino acids. [0003] Phenylalanine is mainly used as a reagent and parenteral nutrition infusion in medicine, and also used as an ingredient in nutritional supplements such as synthetic diets and essenti...

AI Technical Summary

Problems solved by technology

The yield of the chemical synthesis method is low; it needs to use highly toxic cyanide, which pollutes the environment; there are many side reactions; the process route is long and other disadvantages

The microbial fermentation method has the advantages of being environmentally friendly, energy-saving and green, but the disadvantage is that the concentration of fermentation products is low, the production cycle is long, and the process management requirements are strict.

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