Method for extracting DNA from colla corii asini

Abstract

The invention provides a method for extracting DNA from colla corii asini. As colla corii asini is a substance which is high in protein component and great in viscidity, the DNA is hard to extract. In the preparation process of colla corii asini, raw materials are repeatedly decocted, so that the part of DNA is destroyed, and the difficulty in extracting the DNA of colla corii asini is further increased. According to the method provided by the invention, the optical time and optimal use level of proteinase K which acts on. The method comprises the following steps: digesting by virtue of lauryl sodium sulfate; carrying out extraction by virtue of balanced phenol, chloroform and isoamylol; separating out the DNA by virtue of 96% ethanol and potassium acetate; and finally, washing by virtue of ethanol and drying. The high-quality DNA of colla corii asini can be successfully extracted by virtue of the method, and the method can be applied to molecular identification of colla corii asini. The method is simple, economic and easy to operate.

Description

technical field [0001] The invention relates to a method for extracting DNA, in particular to a method for extracting DNA from donkey-hide gelatin. Background technique [0002] Donkey-hide gelatin is the donkey skin of the chordate mammalian equine animal. It is a solid gelatin block made by decocting and concentrating. It tastes sweet and flat. It enters the lung, liver, and kidney channels. It has a history of more than 2000 years. Due to the unique efficacy of donkey-hide gelatin, the supply is in short supply, and substitutes made from other animal skins have appeared in various places, which has brought confusion to the pharmaceutical market. In order to identify the ingredients of donkey-hide gelatin sold in the market, the most cutting-edge identification method is molecular identification, which is to extract the DNA of donkey-hide gelatin, and use molecular methods to detect the animal source of the extracted DNA, so as to determine the authenticity of donkey-hide...

AI Technical Summary

Problems solved by technology

However, because donkey-hide gelatin itself is a substance with high protein content and high viscosity, it is not easy to extract DNA. In addition, during the production of donkey-hide gelatin, the raw materials are repeatedly boiled, resulting in partial damage to the DNA, which increases the difficulty of DNA extraction from donkey-hide gelatin.

[0003] At present, the method for extracting DNA from donkey-hide gelatin is mainly based on the industry standard SDS method (the People's Republic of China Agricultural Industry Standard NY / T 674-2009). After experiments, the DNA extraction method has a very low extraction rate of DNA in donkey-hide gelatin samples, often in the When extracting protein, the DNA is taken away together, resulting in poor results of DNA extraction, and subsequent experimental work cannot be carried out

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