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Isolated protein powder prepared by euphausia superba powder

A technology of Antarctic krill powder and protein isolate, which is applied in the field of protein isolate powder, can solve the problem of high salt content and achieve the effects of high protein content, good solubility and high product yield

Active Publication Date: 2014-12-10
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein gel obtained in this way is generally thermally irreversible and has a high salt content

Method used

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  • Isolated protein powder prepared by euphausia superba powder
  • Isolated protein powder prepared by euphausia superba powder
  • Isolated protein powder prepared by euphausia superba powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Take 10 kg of Antarctic krill powder, add 120 L of 90% ethanol to the krill powder, stir in the reaction kettle at room temperature for 10 hours, centrifuge to take the precipitate, and dry it in vacuum to obtain defatted krill powder;

[0040] The protein content in Antarctic krill powder is 60-65%, the lipid content is 8-13%, the ash content is 10-15%, the fluorine content is 1800-2200mg / kg, and the defatted krill powder obtained in step 1) is medium-fat Quality content is less than 2%.

[0041] The determination method of protein content refers to the Kjeldahl method in GB5009.5-2010 "National Standard - Determination of Protein in Food"; the determination method of ash content refers to GB5009.4-2010 "National Standard - Determination of Ash Content in Food"; The determination method of the content refers to the fluoride ion selective electrode method in GB / T5009.18-2003 "National Standard - Determination of Fluorine in Food"; the determination method of the tota...

Embodiment 2

[0055] 1) Take 10 kg of Antarctic krill powder, add 80 L of 95% ethanol to the krill powder, stir in the reaction kettle for 10 hours, centrifuge to take the precipitate, and dry it in vacuum to obtain defatted krill powder;

[0056] The lipid content in the defatted krill powder is less than 2%, and the determination method of the total lipid content adopts the chloroform methanol method.

[0057] 2) Take 5 kg of defatted krill powder, add 50 L of 0.3M acetic acid solution to it, stir in the reaction kettle at room temperature for 4 hours, centrifuge at 5000 r / min to collect the precipitate, wash the precipitate repeatedly with distilled water until neutral, and obtain precipitate A;

[0058] 3) Add 50L of 0.15M sodium hydroxide solution to the precipitate A, stir in a reaction kettle at 40°C for 40min, and centrifuge to get the supernatant A;

[0059] 4) Add 2M HCl to the supernatant A, adjust the pH of the supernatant A to 4.6, let stand at 4°C for 30 minutes, and centrifug...

Embodiment 3

[0066] Antarctic krill protein isolate powder in the present invention has low-fat, low-salt and thermoreversible gel properties, and can be used in food processing as a fat substitute, such as: mayonnaise, processed meat, ice cream, cheese, sauce, dessert, Baked food can be used to replace part or all of fat, effectively regulating and controlling fat intake.

[0067] Take 1kg of Antarctic krill protein isolate, add 10L of water to the protein isolate powder, stir and mix well, then let the protein isolate solution stand at 4°C for 12h; Mix evenly, heat-treat the mixed solution for 30 minutes at 80°C (without stirring during the heat treatment), immediately ice-bath for 10 minutes after heat treatment, and after cooling, use a homogenizer to micronize at 12000r / min for 15 minutes, namely The fat substitute gel is obtained, and the fat substitute powder is obtained after the fat substitute gel is freeze-dried. The results of use show that the protein isolate powder prepared i...

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Abstract

The invention aims at providing isolated protein powder prepared by euphausia superba powder. A method comprises the following operation steps that the euphausia superba powder is degreased by ethanol, and then sequentially subjected to acid treatment, mineral substance removal, alkali treatment, protein dissolving, isoelectric point protein deposition, protein washing with distilled water, fluorine and salt removal and the like; low-fat, low-fluorine and low-salt euphausia isolated protein wet residue is obtained; the distilled water is added to the isolated protein wet residue; a pH (potential of hydrogen) value of a protein solution is adjusted to be neutral; an isolated protein solution is obtained; the isolated protein solution is subjected to spray drying; and then the euphausia superba isolated protein powder is obtained. Raw materials adopted by the method are rich, easy to obtain and high in protein content; the method is simple in preparation technology step, low in equipment investment, high in product yield and environmental-pollution-free, and is suitable for industrial production; the protein content in the euphausia superba isolated protein powder is greater than 90%; the fluorine content is less than 6mg / kg; the ash content is less than 5%; no lipid is remained; and the isolated protein powder is low-fat, low-fluorine and low-salt protein powder, and has the characteristic of forming heat-reversible protein gel.

Description

technical field [0001] The invention belongs to the technical field of protein powder preparation, and in particular relates to an isolated protein powder prepared from Antarctic krill powder. Background technique [0002] Antarctic krill is a class of marine invertebrates distributed in Antarctic waters. According to statistics, the reserves of Antarctic krill are 650-100 million tons, which is a huge treasure house of human protein resources. The protein content of krill dry weight is about 65-75%. Its amino acid composition is reasonable and its essential amino acid content is high. It is an important raw material for high-value protein utilization. And using the property that protein is easy to dissolve in alkaline environment and precipitate at the isoelectric point, return the protein in Antarctic krill raw material in the form of isolated protein, which is a commonly used processing technology in the food industry. [0003] Gel property is one of the most important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04
Inventor 薛长湖王彦超薛勇常耀光魏东芝王玉明徐杰
Owner OCEAN UNIV OF CHINA
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