Method for preparing theabrownin and low-molecular-weight tea polysaccharides by fermenting green tea as raw material

Abstract

The invention provides a method for preparing theabrownin and low-molecular-weight tea polysaccharides by solid state fermentation of green tea as a raw material by adopting aspergillus niger and trametes trogii. The method comprises the following steps: inoculating, performing solid state fermentation, extracting, performing separation and purification, drying and the like. Specifically, the method comprises the following concrete steps: spraying water containing aspergillus niger and trametes trogii into crushed green tea, and fermenting in a room-temperature environment to obtain a fermented material; adding water into the fermented material, performing heating extraction, and filtering extraction liquid through gauze and a microfiltration membrane to obtain filter liquor; performing heat evaporation concentration or vacuum concentration on the filter liquor, adding an edible alcohol aqueous solution into concentrated liquid, fully uniformly mixing, standing, centrifuging, and collecting supernatant and precipitate after centrifugation respectively; performing heat evaporation concentration or vacuum concentration on the supernatant, filtering through an ultrafiltration membrane, and performing spray-drying or vacuum freeze-drying on filter liquor to obtain solid powder namely theabrownin; dissolving precipitate generated after centrifugation by using water, and spray-drying or freeze-drying to obtain low-molecular-weight tea polysaccharides.

Description

technical field [0001] The invention belongs to the fields of tea deep processing and food biotechnology, and specifically relates to a method for preparing theabrownin and low-molecular-weight tea polysaccharides by using green tea as a raw material and fermenting Aspergillus niger and Trametes trichotillobacter. [0002] Background technique [0003] my country is a big country in green tea production, consumption and trade. In 2012, the output of green tea reached 1.179 million tons, accounting for 67% of the total output of tea. At present, my country's tea garden area accounts for half of the world's total tea garden area, the total tea output accounts for about one-third of the world's total tea output, and the tea export volume accounts for about one-fifth of the world's total export volume, of which 70% is green tea. In recent years, the output of tea has been overcapacitated and oversupplied. Especially for green tea, which is the main tea category in my country, t...

AI Technical Summary

Problems solved by technology

Tea polysaccharide is a heteropolysaccharide complex with large molecular weight and unstable solution system, which is not conducive to the absorption of organisms and the development of functional activities. If it is directly injected into the body, it may also cause greater toxicity, which greatly limits the application of tea polysaccharides.