Unlock instant, AI-driven research and patent intelligence for your innovation.

A powdery mildew resistance-related protein, its coding gene and its application

An encoding gene, powdery mildew technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve problems such as plant yield reduction, withering, plant growth and development effects, etc., to achieve the effect of variety improvement and pathogen resistance improvement

Inactive Publication Date: 2017-05-10
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The growth and development of plants infected by powdery mildew will be greatly affected, resulting in reduced yield or even death of plants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A powdery mildew resistance-related protein, its coding gene and its application
  • A powdery mildew resistance-related protein, its coding gene and its application
  • A powdery mildew resistance-related protein, its coding gene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1, Preparation of powdery mildew resistance-related gene AT5G05190

[0032] (1) Screening of mutant Arabidopsis thaliana with enhanced resistance to powdery mildew

[0033] The wild-type Arabidopsis Col-0 was mutated with 0.03% ethyl methanesulfonate (EMS), and the mutants with enhanced resistance to powdery mildew were screened from the mutagenized population. The specific screening method is as follows: under a growth environment of 8000 lux and a relative humidity of 70% in short light (9 hours of light / 15 hours of darkness), use powdery mildew (G.cichoracearum UCSC1) (Adam et al., 1999) on the growth Four weeks of wild type and mutants were inoculated, and the experimental results were observed ( figure 1 ). The experimental results showed that: 7 days after inoculation, unlike the wild type that produced a large number of powdery mildew conidia, the hyphae and conidiophores could not grow on the surface of the leaves of the mutants, and the leaves of the ...

Embodiment 2

[0049] Example 2. Functional verification of powdery mildew resistance-related gene AT5G05190

[0050] (1), reverse mutation experiment

[0051] Digest the PCR amplification product obtained in Example 1 with Xba1 and Sal1; digest pCAMBIAL1300 with Xba1 and Sal1, and collect the large fragment of the vector; connect with T4 ligase at 16°C, transform DH10b competent cells, and find no base mutation by sequencing After extracting the plasmid (Sambrook, 1989), the exogenous gene sequence inserted in the plasmid is shown in SEQ ID No.1, and then the freeze-thaw method is used to transform the Agrobacterium competent GV3101, and then the Agrobacterium is used for flower drop dipping (Clough and Bent, 1998) transformed the mutants screened in Example 1, and screened transgenic plants with hygromycin to obtain 25 positive plants. These 25 positive plants were inoculated with powdery mildew, and 22 transgenic plants reverted to wild-type phenotype ( figure 2 b).

[0052] (2), AT5G...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses powdery mildew resistance associated protein AT5G05190, encoding gene thereof and application of the both. The protein AT5G05190 is protein possessing one of the following amino acid residue sequences: 1) an amino acid residue sequence shown as SEQ ID No. 2 in sequences; and 2) protein which is derived from the amino acid residue sequence 1), is associated with the plant powdery mildew resistance and is obtained by substituting and / or deleting and / or adding one or more amino acid residues on the basis of the amino acid residue sequence shown as SEQ ID No. 2 in sequences. By reducing expression of the protein or the encoding gene, improvement on pathogenic bacteria resistance of plants and improvement on variety are realized, and important theoretical and practical significance is provided for agricultural production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a powdery mildew resistance-related protein, its coding gene and its application. Background technique [0002] Plants grow in nature and are threatened by various plant diseases. Recognition and resistance to infection by pathogenic bacteria is critical for the survival of multicellular organisms (Aderem and Ulevitch, 2000). Receptors in animals, such as Toll-like receptors, can induce innate immune responses by recognizing pathogen ligands. Unlike animals, plants can only rely on each cell's own innate immune system and Whole-plant non-infected tissues elicited by local resistance responses resist subsequent infection by a broad spectrum of pathogens (Cao et al., 1997). [0003] In order to adapt to the environment, plants have gradually formed a multi-level defense system similar to that of animals, forming their own innate immune system (innate immunity). It mainly includes bas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29A01H5/00
CPCC07K14/415C12N15/8205C12N15/8282
Inventor 武广珩刘斯穆赵婷陈永芳李娟唐定中
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI