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Method for detoxification micropropagation of callicarpa nudiflora

A technique of nudibranch and micropropagation, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., and can solve problems such as poor genetic stability, low multiplication efficiency, and long reproductive cycle of nudibranch

Active Publication Date: 2014-12-24
JIUZHITANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of long reproductive cycle, low multiplication efficiency, high cost and poor genetic stability of the nudibranch, and provide a low-cost, stable and high multiplication efficiency virus-free microbiota. traditional method

Method used

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  • Method for detoxification micropropagation of callicarpa nudiflora
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  • Method for detoxification micropropagation of callicarpa nudiflora

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specific Embodiment approach 1

[0065] Specific embodiment one: the detoxification and micropropagation method of the nudibranch in this embodiment is realized according to the following steps: 1. with the mass concentration of the nudibranch fruit explant on the ultra-clean workbench after cleaning. Soak in 75% ethanol solution for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercuric chloride solution for 12 minutes, and then rinse with sterile water for 5 times to obtain sterile explants ;

[0066] ②Then the seeds were stripped off and inoculated on the modified MS solid medium prepared with 1 / 2 MS medium and 100.0 mg / L inositol as the basic medium, which also contained 0.01 mg / L gibberellin and 0.01 mg / L NAA, 3.0% sucrose, pH value 5.6-6.0, and then cultured for 13-15 days under the conditions of temperature 28 ℃, humidity 70%, light intensity 100 lx, and light 8 h / d. d, to obtain the aseptic buds of Aurora nudiflora, the length of which is 0.5-1.0 cm;

[0067] ③Transfer ...

Embodiment 2

[0074] The 0.1% mercuric chloride solution disinfection in step 1. is 15min, and other steps are with embodiment 1.

[0075] In this example, the proliferation rate of the explants of A. nudiflora was 91.4%, and the emergence time of sterile seedlings was 13 days ± 2 days. The growth of the plants in the multiplication culture is good, the monthly multiplication rate is 5.15, the rooting rate in the rooting culture is 100%, the uniformity of the nudibranch seedlings is high, and the survival rate of transplanting after hardening is 98.5%.

Embodiment 3

[0077] The gibberellin concentration in step 2. is 0.03 mg / L, the NAA concentration is 0.03 mg / L, and other steps are the same as in Example 1.

[0078] In this example, the proliferation rate of the explants of A. nudiflora was 90.1%, and the emergence time of sterile seedlings was 13 days ± 2 days. The cultivation effect of strong seedlings was good. The growth of the plants in the multiplication culture is good, the monthly multiplication rate is 5.05, the rooting rate in the rooting culture is 100%, the uniformity of the nudibranch seedlings is high, and the survival rate of transplanting after hardening is 98.1%.

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Abstract

The invention provides a method for detoxification micropropagation of callicarpa nudiflora, and relates to solve the problems of long propagation period of the callicarpa nudiflora, low propagation efficiency and high cost by using a method of plant detoxification micropropagation and tissue culture. The method comprises the steps of (1) detoxification of explants; (2) culture of aseptic seedlings; (3) culture of micro branches: transplanting proliferated aseptic seedlings into a seedling-strengthening culture medium to culture; (4) propagation culture: cutting the strengthened micro branches into segments with one apical bud or 1-2 axillary buds for culture; (5) rooting culture: transplanting the micro branches to a rooting culture medium when the growth length of the micro branches reaches 2.0-3.0 cm; (6) hardening culture: hardening the rooting cultured callicarpa nudiflora seedlings in a sunshelter for 7-10 d; and (7) transplanting callicarpa nudiflora seedlings after hardening of the seedlings into seedling cups to finish detoxification micropropagation of the callicarpa nudiflora. The method for the detoxification micropropagation of the callicarpa nudiflora has the advantages of short propagation cycle, high propagation rate, identical seedling characteristics, low cost, and high rooting rate and survival rate of transplanted seedlings. Besides, the growth cycle is not restricted by seasons.

Description

technical field [0001] The invention relates to a technique for tissue culture and rapid propagation of the nude flower purple pearl, and the invention belongs to the fields of seed seedlings, agricultural biotechnology and medicinal plant cultivation. [0002] Background technique [0003] Naked flower purple beads ( Callecarpa. nudiflora Hook. et Arn) is a plant of the genus Verbenaceae, and its dried leaves are used as medicine, which was recorded in the 1977 edition of "Chinese Pharmacopoeia". It has the effects of stopping bleeding, dispelling blood stasis, antibacterial and anti-inflammation, and is an important ingredient of nearly 40 kinds of Chinese patent medicines, such as nudehuazizhu tablets, nudehuazizhu dispersible tablets, nudehuazizhu capsules, nudehuazizhu granules, and nudehuazizhu suppositories. raw material. As the naked flower purple pearl is an important medical raw material, wild resources are severely damaged, and the collection of wild resources...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张影波于福来李伟庞玉新谷陟欣黄胜徐向平王丹官玲亮袁莉
Owner JIUZHITANG
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