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New process for extracting heparin by degrading lung tissues

A technology of lung tissue and new technology, applied in the field of bioengineering

Active Publication Date: 2014-12-24
揭阳市润达肠衣有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of fully degrading pig lungs and bovine lungs without destroying their physiologically active components, the present invention provides a biodegradation method with mild conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] First, raw material processing: take 1 portion of fresh pig lung and bovine lung, remove fat and bronchi, soak in water for 60 minutes, wash with water to remove red blood cells, mash into a paste.

[0020] Further, enzymatic hydrolysis: take the paste lung pulp, add 4 times the saline, the concentration of the saline is 1.8%-3.8%, raise the temperature to 40±2°C, adjust the pH to 8.0-9.0, add half of the total amount of the activating enzyme solution, Stir well, slowly raise the temperature to 58±2°C, enzymatically hydrolyze for 1 hour, add 0.6‰ sodium dodecylsulfonate (SDS), stir slowly, then add the remaining activating enzyme solution, and enzymolyze for 2 hours until the system is free solid matter. During the enzymatic hydrolysis process, maintain the enzymatic hydrolysis temperature at 58±2°C and pH 8.5-9.0.

[0021] Wherein, the preparation method of activating enzyme use liquid is as follows: a) take by weight percentage 3% of alkaline protease powder, 3% of p...

Embodiment 2

[0030] First, raw material processing: take 1 portion of fresh pig lung and bovine lung, remove fat and bronchi, soak in water for 60 minutes, wash with water to remove red blood cells, mash into a paste.

[0031] Further, enzymatic hydrolysis: take the paste lung pulp, add 8 times the saline, the concentration of the saline is 1.8%-3.8%, raise the temperature to 40±2°C, adjust the pH to 8.0-9.0, add half of the total amount of the activating enzyme solution, Stir well, slowly raise the temperature to 58±2°C, enzymatically hydrolyze for 1 hour, add 1‰ sodium dodecylsulfonate (SDS), stir slowly, then add the remaining activating enzyme solution, enzymolyze for 2 hours, until the system is free solid matter. During the enzymatic hydrolysis process, maintain the enzymatic hydrolysis temperature at 58±2°C and pH 8.5-9.0.

[0032] Wherein, the preparation method of activating enzyme use liquid is as follows:

[0033] a) Weigh 5% of alkaline protease powder, 5% of pectinase powder...

Embodiment 3

[0042] First, raw material processing: take 1 portion of fresh pig lung and bovine lung, remove fat and bronchi, soak in water for 60 minutes, wash with water to remove red blood cells, mash into a paste.

[0043] Further, enzymatic hydrolysis: take the paste lung pulp, add 6 times the saline, the concentration of the saline is 1.8%-3.8%, raise the temperature to 40±2°C, adjust the pH to 8.0-9.0, add half of the total amount of the activating enzyme solution, Stir well, slowly raise the temperature to 58±2°C, enzymatically hydrolyze for 1 hour, add 0.8‰ sodium dodecylsulfonate (SDS), stir slowly, then add the remaining activating enzyme solution, and enzymolyze for 2 hours until the system is free solid matter. During the enzymatic hydrolysis process, maintain the enzymatic hydrolysis temperature at 58±2°C and pH 8.5-9.0.

[0044] Wherein, the preparation method of activating enzyme use liquid is as follows:

[0045] a) Weigh 3% of alkaline protease powder, 5% of pectinase pow...

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PUM

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Abstract

The invention relates to the field of bioengineering and specifically relates to a new process for extracting heparin by degrading lung tissues. The new process comprises the steps of mincing raw materials pig lung and bovine lung removed from fat and bronchia, preparing a compound enzyme agent and adding a coenzyme component to obtain an activating enzyme working solution, and performing enzymatic hydrolysis on the pig lung and the bovine lung by use of the activating enzyme working solution. The tissues of the pig lung and the bovine lung can be digested into water-soluble components. As the degradation conditions are mild, the active ingredients in the pig lung and the bovine lung are remained completely so that the ingredients such as heparin, protein and polypeptides can be separated conveniently.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to a method for degrading biological tissue, in particular to a new process for degrading and extracting heparin from lung tissue. Background technique [0002] Porcine and bovine lungs contain heparin, an anticoagulant. Heparin is a mucopolysaccharide sulfate composed of glucosamine, L-iduroside, N-acetylglucosamine and D-glucuronic acid alternately, with an average molecular weight of 15KD and strong acidity. Heparin binds to proteins in tissues. A single protease degradation method is generally adopted at home and abroad. This method can only partially degrade pig lung and bovine lung tissue, so that the yield of heparin, peptides, and protein active components in the raw materials will be reduced in subsequent routine separations. The extracted heparin sodium has a potency of 10-30IU. / MG range, the possibility of mass production is reduced. In addition, some researchers have adop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 刘初亮利光辉陈洁辉刘佳华
Owner 揭阳市润达肠衣有限公司
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