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Method for utilizing terahertz time-domain spectroscopy technology to detect amino acid content in grain

A terahertz time-domain and spectral technology, which is applied in the field of food component detection, can solve the problems of weak near-infrared spectrum penetration, spectral peak width, and weak overlapping absorption intensity, and achieve accurate evaluation and prediction capabilities and stability, fast Accurate quantitative detection and prediction of the effect of small mean square error

Inactive Publication Date: 2014-12-24
CAPITAL NORMAL UNIVERSITY +2
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Problems solved by technology

However, the above-mentioned method of using near-infrared spectroscopy to detect the content of amino acids in peanuts has the following problems: the near-infrared spectroscopy is based on the multiplication and combined frequency absorption of internal molecular vibrations, the spectral peaks are wide, the overlap is serious, and the absorption intensity is weak
At the same time, the penetrability of the near-infrared spectrum is relatively weak, and it cannot penetrate the coating or outer packaging with a certain thickness. destroy the integrity of the sample

Method used

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  • Method for utilizing terahertz time-domain spectroscopy technology to detect amino acid content in grain
  • Method for utilizing terahertz time-domain spectroscopy technology to detect amino acid content in grain
  • Method for utilizing terahertz time-domain spectroscopy technology to detect amino acid content in grain

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Embodiment

[0037] This example provides a method for analyzing the content of L-glutamic acid in grain or food by using terahertz time-domain spectroscopy. Wherein, after adding a known amount of L-glutamic acid (purchased from Sigma Company) to rice powder (provided by the Inspection and Quarantine Bureau of Zhangjiakou City, Hebei Province), the mixed samples to be tested with different L-glutamic acid concentrations were prepared, and the The sample to be tested adopts the method of the present invention to detect and analyze as a blind sample, and the specific steps are as follows:

[0038] (1) Put the rice into a pulverizer to pulverize it, sieve it after grinding (100 mesh, particle size ≤ 150 μm), put it into an oven and dry it to obtain rice powder; mix the rice powder and L-glutamic acid powder according to Mix in different mass ratios, transfer to an agate mortar and further grind to obtain the fine powder of the mixed sample to be tested, wherein the mass percentage of L-gluta...

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Abstract

The invention provides a method for utilizing a terahertz time-domain spectroscopy technology to detect amino acid content in grain. The method comprises the following steps: grinding and then performing tableting on a grain sample to be detected, directly utilizing a terahertz time-domain spectroscopy system to detect the sample subject to tableting in a transmission measurement mode and under the nitrogen condition to obtain terahertz time-domain spectroscopy signals as sample signals, under the same conditions, acquiring the terahertz time-domain spectroscopy signals under the nitrogen condition as reference signals, then obtaining an absorptivity spectrum of the grain tableting sample to be detected, at last dividing the absorptivity spectrum of the grain tableting sample to be detected into a calibration set sample absorptivity spectrum and a verification set sample absorptivity spectrum, utilizing a partial least squares regression method to establish a quantitative analysis mode, and obtaining the quantitative detection value of the grain sample to be detected. The method disclosed by the invention is simple in preparation of the sample, is not required to process any pretreatment, and can practically and effectively realize fast and accurate quantitative detection on the amino acid in the grain or food.

Description

technical field [0001] The invention relates to a method for detecting amino acid content in grains using terahertz time-domain spectroscopy technology, and belongs to the technical field of food component detection. Background technique [0002] Amino acid is an important nutrient for human growth, and it is the basic unit that constitutes polypeptides and biologically functional macromolecular proteins in the body. Amino acids not only have unique physiological functions, but also have unique functions in the food industry. Amino acids that are widely used in the food industry include glutamic acid, lysine, threonine, tryptophan, and arginine. Among them, L-glutamic acid is mainly used in the production of monosodium glutamate, spices, etc. due to its unique taste, and L-glutamic acid is also an important nutritional supplement and biochemical reagent, which itself can be used as a drug to participate in the production of proteins in the brain And sugar metabolism, promo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3586
Inventor 张卓勇陈泽炜杨玉平
Owner CAPITAL NORMAL UNIVERSITY
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