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Method for preparing glucuronolactone by using glycyrrhetinic acid fermentation waste liquid

A technology of glucuronolactone and glucuronic acid, which is applied in the field of preparation of glucuronolactone, and achieves the effects of simple process steps, no decolorization process, and good product quality

Active Publication Date: 2014-12-31
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In a word, the method of preparing glucuronolactone from glycyrrhetinic acid fermentation waste liquid has very practical significance, and there is no relevant report on this preparation method in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] With fermentation medium: 3% monoammonium glycyrrhizinate, 0.2% urea, 0.025% KH 2 PO 4 , 0.01% MgSO 4 , sterilized at 121°C for 15 minutes and then cooled to room temperature; take a fresh PDA slant containing highly active β-glucuronidase-transformed strains, and use an inoculation spoon to inoculate the fermentation medium; set the temperature at 28°C, and stir for 5 days until the substrate is completely transformed; Filtrate the fermentation broth to remove bacteria and glycyrrhetinic acid precipitation; the filtered fermentation broth flows through the pre-treated OH type D311 weakly basic anion exchange resin at a flow rate of 2BV / h to exchange and adsorb glucuronic acid; wash with 0.5% ammonia water Desorb glucuronic acid; add absolute ethanol to the eluent and concentrate under reduced pressure at 50°C to 60°C until no visible water distills out; add glacial acetic acid with a mass fraction of 40% and acetic anhydride with a mass fraction of 3% to the concentra...

Embodiment 2

[0025] With fermentation medium: 2% monoammonium glycyrrhizinate, 0.2% starch, 0.4% urea 3, 0.05% KH 2 PO 4 , 0.02% MgSO 4 , sterilized at 121°C for 15 minutes and then cooled to room temperature; take a fresh PDA slant containing highly active β-glucuronidase-transformed strains, and use an inoculation spoon to inoculate the fermentation medium; set the temperature at 30°C, and stir for 4 days until the substrate is completely transformed; Filtrate the fermentation broth to remove bacteria and glycyrrhetinic acid precipitation; the filtered fermentation broth flows through the pre-treated OH-type D301 weakly basic anion exchange resin at a flow rate of 2BV / h to exchange and adsorb glucuronic acid; wash with 0.8% ammonia water Desorb glucuronic acid; add absolute ethanol to the eluent and concentrate under reduced pressure at 50°C to 60°C until no visible water distills out; add glacial acetic acid with a mass fraction of 40% and acetic anhydride with a mass fraction of 3% to...

Embodiment 3

[0027] With fermentation medium: 4% monoammonium glycyrrhizinate, 0.4% NaNO3, 0.05% KH 2 PO 4 , 0.02% MgSO 4, sterilized at 121°C for 15 minutes and then cooled to room temperature; take a fresh PDA slant containing highly active β-glucuronidase-transformed strains, and use an inoculation spoon to inoculate the fermentation medium; set the temperature at 28°C, and stir for 6 days until the substrate is completely transformed; Filtrate the fermentation broth to remove bacteria and glycyrrhetinic acid precipitation; the filtered fermentation broth flows through the pre-treated OH type D301 weakly basic anion exchange resin at a flow rate of 3BV / h to exchange and adsorb glucuronic acid; wash with 0.8% ammonia water Detoxify glucuronic acid; add absolute ethanol to the eluate and concentrate under reduced pressure at 50°C to 60°C until no visible water distills out; add glacial acetic acid with a mass fraction of 40% and acetic anhydride with a mass fraction of 3% to the concentr...

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Abstract

The invention provides a method for preparing glucuronolactone by using a glycyrrhetinic acid fermentation waste liquid. The method comprises the following steps: inoculating a strain culture containing highly active beta-glucuronidase into a fermentation culture medium containing glycyrrhetate, fermenting to obtain a glycyrrhetinic acid precipitate, carrying out ion exchange resin recovery on glucuronic acid in the obtained fermentation liquid, and lactonizing to prepare glucuronolactone. The method mainly has the advantages of realization of the comprehensive utilization of fermentated glycyrrhizic acid, obtaining of glycyrrhetinic acid and the byproduct glucuronolactone, low energy consumption, no use of strong oxidants polluting the environment, simple steps, no byproducts, omitting of a decolorization process, and good product quality.

Description

technical field [0001] The invention relates to a method for preparing glucuronolactone by using glycyrrhetinic acid fermentation waste liquid, which belongs to the field of medicine and chemical industry. Background technique [0002] Glucuronolactone, referred to as glucuronolactone, the preparation name is Gantaile (commonly known as Gantong), its chemical composition is: D-(+)-furanoglucuronide γ-lactone[3], and its molecular formula is C 6 h 8 o 6 . Its mechanism of action is to change into glucuronic acid under the action of enzymes in the body, and glucuronic acid in the form of glucuronic acid salt or complex can combine with toxic substances such as hydroxyl, carboxyl, and sulfhydryl to form non-toxic or low-toxic It can protect the liver and detoxify. Therefore, it can be used as a liver detoxifier and immune function regulator, and it is a conventional liver-protecting medicine. In addition to the application in the field of medicine, glucuronolactone and its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/04C07H1/00C12P19/00
Inventor 魏元刚杨永安汤文建朱海亮季浩
Owner JIANGSU TIANSHENG PHARMA
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