76 results about "Glucuronates" patented technology

The invention discloses a synthesis method and an intermediate compound of morphine-6-Beta-D-glucuronide. The synthesis method comprises the following steps: (1) carrying out a reaction on 3-acetyl morphine and acyl-protected glucuronate in an organic solvent 1 under the catalysis of lewis acid so as to obtain an intermediate shown as a formula (IV); (2) hydrolyzing the intermediate shown as the formula (IV) with lithium hydroxide and neutralizing the intermediate shown as the formula (IV) with hydrobromic acid in a mixture solvent of C1-C4 alkanol and water; and washing the intermediate with the C1-C4 alkanol and drying, thereby obtaining the intermediate compound of the morphine-6-Beta-D-glucuronide, wherein the definitions of substituent groups in the formula (III) and the formula (IV) are shown in the specification. The synthesis method has the advantages of moderate condition and high yield, and is simple to operate and easy to industrialize.

A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferring activity and N-acetylgalactosamine transferring activity.

The invention discloses a methods used for producing glucuronic acid, and a special-purpose engineering bacteria of the method. A first method comprises following steps: inositol is taken as a raw material, under the effect of the engineering bacteria, glucuronic acid is produced; the engineering bacteria is a recombinant bacteria capable of expressing function protein, and is obtained through introduction of functional genes used for coding the function protein into starting bacteria. A second method comprises following steps: inositol is taken as a raw material, under the effect of the function protein, glucuronic acid is produced; the function protein is one selected from (a1), or (a2), or (a3); (a1) the protein is represented by sequence table 3; (a2), the protein is a fusion protein obtained through connecting the N terminal or / and the C terminal of the protein in (a1) with labels; and (a3), the protein is represented by sequence table 7. Great economical benefit is achieved in glucuronic acid production, and high popularization application value is achieved.

A biodegradable hyaluronic acid derivative comprising a modified hyaluronic acid repeating unit represented by the formula (HA)-[O(C═O)NH-M]p, wherein HA is a unit including N-acetyl-D-glucosamine and D-glucuronic acid, M is a modifying moiety containing a C2-16 hydrocarbyl group or a prepolymer, and p is an integer of 1 to 4. The biodegradable hyaluronic acid derivative when dissolved in a hydrophilic medium can form micelles and can be used to entrap a pharmaceutically active or bioactive molecule.

The invention discloses a substrate for detecting beta-D-glucuronidase. The substrate is specifically thymolphthalein-alpha-D-glucuronic acid. A preparation method comprises the following steps: preparing thymolphthalein-alpha-D-methyl glucuronate through reaction of thymolphthalein and acetyl bromide-alpha-D-methyl gluconate, removing acetyl to obtain the thymolphthalein-alpha-D-methyl gluconate,and then performing hydrolysis reaction, so as to obtain the thymolphthalein-alpha-D-glucuronic acid. As the substrate for detecting the beta-D-glucuronidase, the thymolphthalein-alpha-D-glucuronic acid disclosed by the invention has the advantages of stable property, difficulty in deterioration and sensitivity to development; furthermore, a synthesis process is simple, and the cost can be obviously reduced.

Sebum production inhibitors containing as an active ingredient a compound of general formula (1) below having a glucuronic acid derivative and a glucosamine derivative in the structure or a pharmacologically acceptable salt thereof

The invention discloses a preparation method of quercetin-3-O-β-D-glucuronic acid. It extracts the traditional Chinese medicine barley as a raw material, and the obtained extract is filtered and concentrated, and the ethanol content is adjusted to 40 ~80%, remove impurities, concentrate the supernatant to dryness, dissolve in methanol-water, reverse phase column chromatography, elute with 20-70% methanol, detect by thin chromatography, recrystallize and depigment the fraction containing the target stream, After drying, the pure quercetin-3-O-β-D-glucuronic acid with a purity greater than 99% can be obtained in batches. The invention has the advantages of less consumption of organic solvent, low toxicity, simple operation, low cost, and easy laboratory small-scale preparation and pilot production.

Conjugates of SN-38 that provide optimal drug release rates and minimize the formation of the corresponding glucuronate are described. The conjugates release SN-38 from a polyethylene glycol through a β-elimination mechanism.

The invention discloses a method for quantitative detection of gluconic acids and glucono lactone, and belongs to the technical field of separation and detection. The method mainly adopts an SB-Aq chromatographic column and an amino chromatographic column in a combined manner to qualitatively and quantitatively detect the gluconic acids and the glucono lactone which comprise gluconic acid, glucuronic acid, d-saccharic acid and d-saccharic acid 1,4-lactone. According to the method, firstly, the SB-Aq chromatographic column is adopted to perform direct quantitative analysis on the d-saccharic acid 1,4-lactone in a sample; secondly, the amino chromatographic column is adopted to perform direct quantitative analysis on the glucuronic acid and the d-saccharic acid in the sample; finally, the content of the gluconic acid in the sample is obtained through calculation. The method is simple and convenient in pretreatment, high in sensitivity and capable of determining multiple components, and has important significance of improvement of detection working efficiency, control of kombucha production quality and increase of the functional factor yield.

A novel sweetener extracted from sweet Gynostemma pentaphyllum Makino. has a main structure of ethyl-6-glucuronate-1,4-alpha-D-galactose, and is a natural non-carbohydrate sweetener. A preparation method of the novel sweetener comprises the following steps: selecting sweet Gynostemma pentaphyllum Makino. tea, removing chlorophylls, fats and saponins through an organic solvent, carrying out heat insulation distilled water extraction at 70-100 DEG C for 0.5-2 h, decolorizing obtained extract with H2O2, removing proteins through a Sevage technology, and dialyzing the obtained extract in distilled water through using a dialysis bag with the molecular weight cutoff range of 500-2000 in order to remove most polysaccharide and protein impurities; dialyzing the obtained extract in distilled water through using a dialysis bag with the molecular weight cutoff of 100-500 to remove a small amount of salts and the organic solvent; or directly carrying out water extraction without the organic solvent, and removing impurities through a dialysis technology adopting dialysis bags with different molecular weight cutoffs; and drying the finally obtained extract by using a refrigerating drying machine to obtain a sweetener sample. The sweet Gynostemma pentaphyllum Makino. sweetener has high purity, is a natural novel sweetener, has the sweetness 300-1200 times that of sucrose, and does not insulin metabolism.

A fluorescent probe for measurement of UDP-glucuronosyltransferase, which comprises a fluorescein derivative, wherein in the fluorescein derivative, the 2-carboxy group on the benzene ring of fluorescein is replaced with another monovalent substituent, provided that said substituent is a substituent other than sulfo group, and the substituent does not have carboxy group or sulfo group, and wherein the fluorescein derivative may have an arbitrary substituent at a position on the benzene ring other than the 2-position, and the fluorescein derivative may have a substituent selected from the group consisting of an alkoxy group and a halogen atom at the 2-position and / or the 7-position of fluorescein.