High-efficiency phosphate-solubilizing bacteria and application thereof

A technology of high-efficiency phosphorus-solubilizing bacteria and phosphorus-solubilizing bacteria, applied in the field of microorganisms, can solve problems such as soil water retention decline, grassland degradation, and soil compaction, and achieve the effects of promoting root growth, increasing available phosphorus, and improving soil structure

Active Publication Date: 2015-01-07
轩凯生物科技(山东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extensive use of chemical fertilizers has obviously caused adverse consequences such as soil compaction, soil water retention capacity decline, grassland degradation, and severe desertification

Method used

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  • High-efficiency phosphate-solubilizing bacteria and application thereof
  • High-efficiency phosphate-solubilizing bacteria and application thereof
  • High-efficiency phosphate-solubilizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Prepare the phosphate solubilizing bacteria agent of the present invention as follows:

[0041] Activate the phosphate-solubilizing bacteria Bacillus subtilis JT-1 stored on a slant at 4°C, inoculate it on the culture medium of an eggplant-shaped culture flask (ie, the activation medium), and cultivate it for 18-24 hours. Transfer the well-grown strains to the fermentation medium, culture at 30-35°C for 12-24 hours, and obtain the fermented liquid or the dried product of the fermented liquid. The number of bacteria in the bacterial agent can reach 1×10 10 -9×10 12 CFU / g.

[0042] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation medium component is: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl0.3g / L, MnSO 4 4H 2 O0.03g / L, KH 2 PO 4 1g / L, pH 6.0-8.0.

[0043]During the fermentation and cultivation process of the above-mentioned production tank, the ventilation rate of sterile air is 0.6-0.8vvm, and the stirring speed is ...

Embodiment 2

[0044] Example 2: Determination of the phosphorus solubilizing ability of bacterial strains.

[0045] Add 100mL inorganic phosphorus liquid culture medium respectively in 500mL Erlenmeyer flask, medium composition is glucose 10g / L, (NH 4 ) 2 SO 4 1g / L, MgSO 4 ·7H 2 O0.3g / L, NaCl0.3g / L, FeSO 4 ·7H 2 O0.03g / L, MnSO 4 4H 2 O0.03g / L, Ca 3 (PO 4 ) 2 5.0g / L. Then inoculate 1 mL of bacterial suspension, and do not inoculate control (add 1 mL of sterile water to the culture medium) at the same time, and repeat all treatments 3 times. Shake culture at 30°C and 150rpm for 7 days, centrifuge the culture solution at 6000rpm for 20min, measure the amount of phosphorus solution (water-soluble phosphorus content) by molybdenum antimony colorimetric method, and it is 682.43-725.28mg / L, and the phosphorus solution rate is 68.2% -72.5%. Phosphorus solubilizing bacteria JT-1 has a higher ability to solubilize phosphorus.

Embodiment 3

[0046] Example 3: Phosphorus solubilizing bacteria can improve crop yield.

[0047] The fermentation preparation of phosphate solubilizing bacteria agent is basically the same as embodiment 1.

[0048] Taking the rice in the plot as the experimental object, the test set up 2 treatments, the dosage of which was 30kg / hm without adding the phosphate-dissolving bacteria agent (T1) and adding the phosphate-dissolving bacteria agent (T2) 2 , each treatment was repeated 3 times, the length of the plot was 8m, the width was 3m, and the area of ​​the plot was 24m 2 , Protective rows were set up around the community, except for the application of phosphate solubilizing bacteria, the other management measures such as fertilization and watering were the same for the two treatments.

[0049] Table 1. Effects of each treatment on rice yield and its components

[0050]

[0051] It can be seen from Table 1 that the phosphorus-dissolving bacteria agent can significantly increase the effec...

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Abstract

The invention provides independently screened high-efficiency phosphate-solubilizing bacteria and a preparation method of a microbial inoculum of the phosphate-solubilizing bacteria, and belongs to the technical field of biology. The phosphate-solubilizing bacterial strain is named as Bacillus subtilis JT-1, and is identified as bacillus subtilis, which is preserved at the China Center for Type Culture Collection (CCTCC) on August 5, 2014; and the preservation number is CCTCC M 2014367. The phosphate-solubilizing bacteria can transform insoluble inorganic phosphorus into a high-quality phosphorus compound which can be directly absorbed and utilized by plants, can improve the bio-availability of the insoluble phosphorus in soil and the utilization efficiency of a phosphate fertilizer, can save a fertilizer and increase production, and have important meaning and application value in the aspects of cultivating and developing the soil ecological fertility, and keeping agricultural ecological balance.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a high-efficiency phosphate-solubilizing bacterium and its application. Background technique [0002] Phosphorus is one of the indispensable macroelements for plant growth and development. It is an important component of plants, and participates in various physiological and biochemical processes in plants in various ways, and plays an important role in promoting plant growth and metabolism. However, soil Most of the phosphorus in the plant cannot be directly absorbed by plants. According to surveys, 74% of the cultivated land in China is deficient in phosphorus, and 95% of the phosphorus in the soil is in an invalid form, which is difficult for plants to absorb and utilize. Among them, insoluble organic phosphorus generally accounts for 20%-50% of the total phosphorus in the soil, accounting for insoluble soil phosphorus. 10%-85% of the total phosphorus. The utilization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/125
CPCC05F11/08C12N1/205C12R2001/125
Inventor 徐虹冯小海殷文锋许宗奇李莎
Owner 轩凯生物科技(山东)有限公司
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