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Preparation method of whole-cell catalytic transesterification reaction micro-reactor

A technology of exchange reaction and microreactor, which is applied in the direction of enzyme production/bioreactor, immobilized on/in organic carrier, etc., can solve the problems of limited application, unsatisfactory film-forming permeability, poor mechanical properties, etc. Achieve the effects of uniform particle size, good mechanical properties, and reduced enzyme activity loss rate

Inactive Publication Date: 2015-01-14
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosan is one of the more commonly used microcapsule materials. It has excellent film-forming properties, safety, and biocompatibility, but its poor mechanical properties greatly limit its application as a microreactor for catalytic transesterification reactions. At the same time, the permeability of its film formation can not meet the requirements of microreactors for catalytic transesterification reaction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of preparation method that is used for whole cell catalyzed transesterification reaction microreactor, take chitosan powder (CTS), polyvinylidene fluoride powder (PVDF), sodium alginate, sodium citrate and calcium chloride as raw material, through CTS / PVDF composite membrane microcapsules are prepared through solidification into spheres, sol-gel film formation, and liquefaction into capsules.

[0025] The preparation process comprises the following steps:

[0026] 1) Prepare 2 parts of fermentation broth: 1.0% peptone, 2.0% glucose, 0.1% ammonium sulfate, 0.5% yeast extract, 0.05% magnesium sulfate, 1.0% olive oil, and 0.2% dipotassium hydrogen phosphate. Adjust pH to 8.5 before use, 1×10 5 Pa sterilization for 20min.

[0027] 2) Add sodium alginate to 1 portion of fermentation culture medium, stir evenly and swell to form solution A containing 6% sodium alginate; drop solution A into 5% calcium chloride solution, and calcify for 24 hours , to obtain gel beads...

Embodiment 2

[0034] A kind of preparation method that is used for whole cell catalyzed transesterification reaction microreactor, take chitosan powder (CTS), polyvinylidene fluoride powder (PVDF), sodium alginate, sodium citrate and calcium chloride as raw material, through CTS / PVDF composite membrane microcapsules are prepared through solidification into spheres, sol-gel film formation, and liquefaction into capsules.

[0035] The preparation process comprises the following steps:

[0036] 1) Prepare 2 parts of fermentation broth: 1.0% peptone, 2.0% glucose, 0.1% ammonium sulfate, 0.5% yeast extract, 0.05% magnesium sulfate, 1.0% olive oil, and 0.2% dipotassium hydrogen phosphate. Adjust pH to 8.5 before use, 1×10 5 Pa sterilization for 20min.

[0037] 2) Add sodium alginate to 1 portion of fermentation culture medium, stir evenly and swell to form solution A containing 20% ​​sodium alginate; drop solution A into 15% calcium chloride solution, and calcify for 24 hours , to obtain gel b...

Embodiment 3

[0044] A kind of preparation method that is used for whole cell catalyzed transesterification reaction microreactor, take chitosan powder (CTS), polyvinylidene fluoride powder (PVDF), sodium alginate, sodium citrate and calcium chloride as raw material, through CTS / PVDF composite membrane microcapsules are prepared through solidification into spheres, sol-gel film formation, and liquefaction into capsules.

[0045] The preparation process comprises the following steps:

[0046] 1) Prepare 2 parts of fermentation broth: 1.0% peptone, 2.0% glucose, 0.1% ammonium sulfate, 0.5% yeast extract, 0.05% magnesium sulfate, 1.0% olive oil, and 0.2% dipotassium hydrogen phosphate. Adjust pH to 8.5 before use, 1×10 5 Pa sterilization for 20min.

[0047] 2) Add sodium alginate to 1 portion of fermentation culture medium, stir evenly and swell to form solution A containing 30% sodium alginate; drop solution A into 10% calcium chloride solution, and calcify for 24 hours , to obtain gel bea...

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Abstract

The invention relates to a preparation method of a whole-cell catalytic transesterification reaction micro-reactor and belongs to the technical field of biochemical engineering. The preparation method comprises that a chitosan powder (CTS) / polyvinylidene fluoride (PVDF) composite microcapsule is prepared from CTS powder, PVDF powder, sodium alginate, sodium citrate and calcium chloride as raw materials by curing balling, sol-gel membrane formation and liquefaction capsule-formation. The CTS / PVDF composite microcapsule can be used as a whole-cell catalytic transesterification reaction micro-reactor. The CTS / PVDF composite microcapsule has uniform particle sizes, good mechanical properties and good permselectivity, can be used for fixing lipase-producing bacterium whole-cell and greatly reduces an enzyme activity loss late.

Description

technical field [0001] The invention relates to a preparation method of a micro-reactor for whole-cell catalytic transesterification reaction. The invention belongs to the field of biochemical technology. Background technique [0002] In order to optimize the biological reaction and transfer performance, the miniaturization of the bioreactor is one of the new development directions. Microreactor (also known as microcapsule), as the immobilized carrier of cells, the selective permeability of the outer polymer membrane ensures the material exchange inside and outside the microreactor, while the internal space structure provides a surface for cell attachment, and the outer Stirring can keep the microcapsules in a suspended state and the shear force will not damage the cell viability, and is conducive to the differentiation of cell mass tissue to increase production. More importantly, the active substances produced by the cells inside the microcapsules can be reused to simplify...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12N11/10
CPCC12N11/10C12N11/04C12N11/08
Inventor 唐雪娇李飞飞段雨欣甘兰张志丹章熠凡
Owner NANKAI UNIV
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