Sinorhizobium SCAUs65 and application thereof

A technology of Sinorhizobium, preservation number, applied in Sinorhizobium SCAUs65 and its application fields, can solve the problems of small planting area, scattered planting, low yield, etc., and achieve strong nitrogen fixation ability, good affinity, and strong stress resistance Effect

Inactive Publication Date: 2015-01-14
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on excellent rhizobium bacteria that match the soybean varieties and ecological environment in the southern soybean producing area, Sichuan soybean producing area.
[0005] Soybean in Sichuan has always been a minor cr

Method used

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  • Sinorhizobium SCAUs65 and application thereof
  • Sinorhizobium SCAUs65 and application thereof
  • Sinorhizobium SCAUs65 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation, purification and preservation of Sinorhizobium SCAUs65

[0016] From the soybeans planted on the purple soil in the hilly area of ​​Jingyang District, Deyang City, Sichuan Province, select the large and plump red root nodules on the main roots of robust plants, scrub the root nodules clean, pick off some root barks, dry them with paper and put them away. In a vial filled with anhydrous calcium chloride and covered with absorbent cotton. After soaking the collected root nodules in sterile water for imbibition in the laboratory, soak in 95% ethanol for 30 seconds to eliminate surface tension, then disinfect the surface with 0.1% (m / v) mercury chloride for 5 minutes, and then rinse with sterile water 6 times, in the case of aseptic operation, a single root nodule was clipped and then added with Congo red YAM medium (mannitol 10g, yeast powder 0.8g, KH 2 PO 4 0.25g, MgSO 4 .7H 2 O0.2g, CaCl 2 .6H 2 O0.1g, NaCl0.1g, 1% (m / v) sodium molybdate 2ml, 1...

Embodiment 2

[0019] The back grafting and matching test of embodiment 2 Sinorhizobium SCAUs65

[0020] The backgraft test of Sinorhizobia SCAUs65 was carried out by hydroponics. The soybean variety used in the test was "Nandou 12", a leading variety in Sichuan. ) and harvested after 46 days of planting. After successful back-grafting with "Nandou 12", the compatibility test with other main soybean varieties was carried out, and the hydroponics method was also carried out. The soybean variety used in the test: another main soybean variety "Gongxuan 1" for intercropping ", the main spring soybean variety "Nandou 8", and the main fresh soybean variety "Chunfengzao". After being cultivated in the above-mentioned light chamber for 1 week, they were cultured under normal light and temperature conditions, and harvested 41 days after planting. Regularly supplement sterile micro-nitrogen nutrient solution. Different combinations of Sinorhizobium SCAUs65 and the above-mentioned soybean varieties ...

Embodiment 3

[0030] The stress resistance of embodiment 3 Sinorhizobium SCAUs65

[0031] The stress resistance of Sinorhizobium SCAUs65 was mainly determined by acid and alkali tolerance, salt tolerance and growth greenhouse range. The YMA medium was used as the basal medium, and the YMA plate cultured at pH 7 and 28°C for 7 days was used as the positive control. The above-mentioned YMA slant culture of Sinorhizobium SCAUs65 was scraped and washed with sterile water for use. The point inoculation method was adopted and repeated 3 times. YMA medium was used as the basal medium for the determination of acid and alkali resistance, and the pH value was adjusted with HCl and NaOH. The pH value was 4.0, 5.0, 6.0, 8.0, 9.0, 10.0, 11.0, and 12.0. Salt tolerance determination also uses YMA medium as the base medium, and the strains are spot-inoculated on a plate containing NaCl, and the mass volume fraction of NaCl is 1%, 2%, 3%, 4% and 5%. Plates for acid and alkali resistance and salt resistan...

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Abstract

The invention discloses sinorhizobium SCAUs65 and application thereof. The strain is separated and purified from a fresh soybean nodulation; the strain is collected in the China center for type culture collection on March 14, 2014, the collection number of the strain is CCTCC NO: M2014084, and the strain is classified and named as sinorhizobium fredii. The strain is applied to the production of szechuan soybeans. The sinorhizobium SCAUs65 disclosed by the invention is an excellent broad-spectrum soybean rhizobium strain which has the advantages of high symbiotic nitrogen-fixing capacity, wide applicable range on szechuan soybean variety, inorganic phosphorus dissolving capacity, organic phosphorus dissolving capacity and potassium dissolving capacity and high stress resistance and has good matching affinity with szechuan main planted soybean variety. A soybean is increased in yield more than 38.9% by being inoculated with the sinorhizobium SCAUs65 without applying a nitrogen fertilizer in different ecological regions and achieves the difference to a significance level compared with a soybean which is not inoculated with the sinorhizobium SCAUs65.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Sinorhizobium SCAUs65 and its application. Background technique [0002] Chemical nitrogen fertilizers have made great contributions to increasing crop yields, but they have high production costs, low utilization rates, and pollute the ecological environment. Biological nitrogen fixation has the advantages of low cost, large amount of nitrogen fixation, continuous nitrogen fixation process, and no pollution, which is conducive to the protection of the ecological environment and the sustainable development of agriculture, and is an effective means of saving costs and increasing efficiency in agricultural production. Great efforts are needed to develop environmentally friendly biological nitrogen fixation technologies in food production. The rhizobia-legume nitrogen fixation system is the most efficient in biological nitrogen fixation. Artificial inoculation of rhizobia ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01G31/00C12R1/41
CPCA01G31/00C12N1/205C12R2001/41
Inventor 徐开未陈远学邹兰王科张勇陈晓辉刘静
Owner SICHUAN AGRI UNIV
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