Unlock instant, AI-driven research and patent intelligence for your innovation.
A molecular detection method for rapid identification of flat head anthracnose and its application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A flat-head anthrax and molecular detection technology, applied in the field of molecular biology, can solve the problems of large workload, long test period, affecting the speed of pathogen diagnosis, etc., and achieve the effect of effective prevention and control.
Active Publication Date: 2016-08-24
CHINA AGRI UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The pathogenic bacteria that cause anthrax include multiple species (Colletotrichum spp.) within the genus Anthracnose, among which Colletotrichum truncatum is the dominant species of anthracnose bacteria in many areas of my country. The study found that tebuconazole and myclobutanil had poor inhibitory rate on the growth of C. truncatum mycelium, but had high antibacterial activity on several other common anthracnose pathogens, that is, C. truncatum on tebuconazole and Myclobutanazole is naturally insensitive. If the dominant species that causes anthracnose in the field is flathead anthracnose, other pesticides should be used for disease control in production, and tebuconazole and myclobutanil cannot be used blindly for disease control. Therefore, the The rapid identification and molecular diagnosis of pathogenic bacteria is particularly important
[0004] At present, the identification of the pathogenic bacteria is mainly carried out through the joint tree construction of morphology and multi-gene sequencing. Since it is necessary to select multiple genes for sequencing of the strain, the identification period is long and the practicability is poor.
If the number of strains to be identified is large, the cost of identification will be high, the workload will be heavy, and the test cycle will be long, which will affect the speed of pathogen diagnosis
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0019] Embodiment 1, the sensitivity determination of anthracnose bacteria to tebuconazole and myclobutanil
[0020] The anthrax strains all come from many regions of the country, and the strain types are: C.truncatum, C.gloeosporioides, C.fructicola, C.scoveillie, and C.fioriniae, each with 50 strains.
[0023] 1) tebuconazole and myclobutanil are made into 10 with methanol 5 μg / ml stock solution. For the determination of the sensitivity of capsicum anthracnose bacteria to tebuconazole, tebuconazole was serially diluted to 100 μg / mL, 500 μg / mL, 1000 μg / mL, 2.5×10 3 μg / mL, 5×10 3 μg / mL, 10×10 3 μg / mL, 20×10 3 Concentration gradient of μg / mL; For the sensitivity determination of capsicum anthracnose bacteria to myclobutanil, the test agent myclobutanil was dilut...
[0032] 1. Strains: Three strains are selected for each anthrax, C. truncatum strains are HBLF11, CQ6, SDWC2-17; C. gloeosporioides are SDLY2-1, GDQY13-85, SC8; C. fructicola are GXGL7, GDQY13-96, YNML2 , C.scoveillie for SXCZ3, SXSZ, FJ33, C.fioriniae for SHFX, JL23, QH8
[0033] 2. Method:
[0034] 1) Strain cultivation: use PDA medium (200 g of peeled potatoes, 18 g of glucose, 13 g of agarpowder, and distilled water to make up to 1 L. 121 ° C, 20 min, after damp heat sterilization) to cultivate the capsicum anthracnose bacteria at 28 ° C. The pre-cultured anthracnose bacteria were inoculated on PDA medium covered with cellophane, cultured in the dark at 28°C for 5 days, and the hyphae were collected, frozen in liquid nitrogen and stored at -80°C for extraction of genomic DNA.
[0035] 2) extract the genomeDNA of different kinds of capsicum anthracnose bacteria respectively:
[0036] Take a...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention relates to rapid detection and identification on pepper colletotrichum truncatum which has natural insensitivity on tebuconazole and myclobutanil and application of the colletotrichum truncatum in field disease and insect prevention and treatment, and in particular discloses a molecular detection method for rapidly identifying pepper colletotrichum truncatum for and a special primer. The specific primer pairs consist of DNA molecules as shown in sequences SEQ ID NO:1 and SEQ ID NO:2. The molecular detection method for detecting and identifying pepper colletotrichum truncatum, which is provided by the invention, has the characteristics of rapidness, stability and accuracy, can be also used for detecting and identifying whether colletotrichum truncatum exists in fields or not in a high flux manner, and has important guiding significance for scientific and effective prevention and treatment on anthracnose.
Description
technical field [0001] The invention relates to the molecular detection and identification of anthrax bacteria (Colletotrichum truncatum) and the application of medicament selection in the prevention and treatment of anthrax caused by flathead anthrax, and specifically discloses a rapid identification of flathead anthrax bacteria that are naturally insensitive to tebuconazole and myclobutanil A C. truncatum method and special primers belong to the technical field of molecular biology. Background technique [0002] Anthracnose (Colletotrichum truncatum) is an important pathogen that can infect more than 460 kinds of plants, and can also cause human diseases. The plantdisease caused by this pathogen - anthracnose has been widely reported all over the world, and it gives agricultural production every year cause serious losses. my country is an important producer of peppers in the world. The pepper anthracnose caused by this pathogen is an important disease on peppers in my co...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More