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Use of N-acetyl-5-methoxytryptamine or analogues thereof, for promoting the mechanism of implantation of the embryo and related compositions and culture media

A technology of methoxytryptamine and acetyl, applied in the field of N-acetyl-5-methoxytryptamine or its analogues for promoting the mechanism of embryo implantation, as well as related compositions and culture media, can solve the problem of High risk of endometrial cancer and other issues

Inactive Publication Date: 2015-02-04
ARES TRADING SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] Another determinant to consider is menopause, when melatonin production decreases and, indeed, the risk of endometrial cancer is higher at this time

Method used

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  • Use of N-acetyl-5-methoxytryptamine or analogues thereof, for promoting the mechanism of implantation of the embryo and related compositions and culture media
  • Use of N-acetyl-5-methoxytryptamine or analogues thereof, for promoting the mechanism of implantation of the embryo and related compositions and culture media
  • Use of N-acetyl-5-methoxytryptamine or analogues thereof, for promoting the mechanism of implantation of the embryo and related compositions and culture media

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Example 1 : in vivo experiment

[0116] Materials and methods:

[0117] Melatonin solution for endometrial cleansing

[0118] The melatonin (or N-acetyl-5-methoxytryptamine; CAS No. 73-31-4) used was obtained from Farmalabor in the form of a powder with a designation >99%.

[0119] Two clinical trials were conducted in which melatonin was administered by endometrial washout (concentration 10×10 -9 g / ml):

[0120] 1) Medium Sydney Blastocyst medium, provided by Cook Ireland (Cat. Nos. G20722 and G20929) (Clinical Trial 1). This medium is commonly used to improve the division, differentiation and growth of blastocysts in vitro. The medium contains D-glucose, all 20 essential L-amino acids, L-taurine, gentamicin, human serum albumin, calcium lactate, calcium pantothenate; sodium chloride, sodium bicarbonate and sodium pyruvate; Potassium Chloride, Potassium Phosphate; Magnesium Chloride, Magnesium Sulfate; Pure Water. Other characteristics of the medium:

[0121...

Embodiment 2

[0190] Example 2 : In vitro studies

[0191] Materials and methods

[0192] The melatonin and media used were the same as those used for the in vivo studies.

[0193] Electron Microscope (SEM)

[0194] A field emission high-resolution electron microscope, FE HITACHI S 4000model, operating at 15-20KW was used.

[0195] sample preparation

[0196] Immediately after removal from the patient, small pieces of endometrial tissue approximately 1 mm in size were placed in fixative buffered with 0.1 M cacodylate containing 1% paraformaldehyde and 0.5% glutaraldehyde, pH 7.2 , placed for 3 hours.

[0197] After the fixation was completed, the tissue pieces were washed in PBS solution for 20 minutes, three times (1 hour in total), and then post-fixed in a solution containing 2% osmium tetroxide and 2.5% potassium ferrocyanide.

[0198] The prepared samples were kept in the dark for 3 hours, and then washed carefully in PBS by swirling, changing the solution every 20 minutes for 4 ...

Embodiment 3

[0211] Example 3 : Effect of melatonin and its analogues on oxidation and apoptosis of endometrial cell line HEC-1-A

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PUM

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Abstract

The present invention refers to the use of N-acetyl-5-methoxytryptamine (melatonin) and / or an analogue thereof, for use in the medical or veterinary field in the assisted reproduction for promoting the mechanism of implantation of the embryo, and in particular for the prevention of implantation failure into the uterus, by topical administration of an effective amount in a mammalian subject female in need of such treatment, and related compositions, culture media and medical devices.

Description

technical field [0001] The present invention relates to the application of N-acetyl-5-methoxytryptamine (melatonin) and / or its analogues, as well as related compositions, culture media and medical devices, said N-acetyl-5-methoxytryptamine Oxytryptamine (melatonin) and / or analogs thereof are useful for promoting the mechanism of embryo implantation in assisted reproduction in the medical or veterinary field by topical administration of an effective amount to a female mammalian subject in need of treatment, In particular, it is used to prevent failure of implantation in the uterus. Background technique [0002] Human embryo implantation into the uterus is a complex mechanism involving both the embryo and the endometrial epithelium. The stages of apposition, adhesion, and invasion involve a variety of molecules that play unique roles in the process, and the molecular dialogue between pregnancy and the endometrium implies intercellular and cellular and biochemical factors int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/165A61K31/4045C12N5/00A61P15/08A61K9/00A61B17/42A61K9/08
CPCA61K9/0039A61K31/165C12N5/0682A61B2017/4216A61K31/4045C12N2501/825A61B17/42A61K2300/00A61P15/06A61P15/08C12N5/00A61B17/435A61K9/0034
Inventor 尼科莱塔·马克西亚
Owner ARES TRADING SA
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