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Production method of gastrodia elata associated honey fungus strain

A production method and technology of accompanying bacteria, applied in the field of microorganisms, can solve the problems of easy aging of bacteria, long culture period, slow growth, etc., and achieve the effects of low cost, shortened production cycle, and strong stamina

Inactive Publication Date: 2015-02-11
关岭自治县丰硕食用菌农民专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of this invention is to provide a kind of preparation method of Gastrodia elata associated bacterium Armillaria bacterial classification, when to solve existing cultivation Armillaria bacterial classification, bacterial classification is easy to aging, stamina is insufficient, growth is slow, and the cultivation period long technical issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Collect chestnut tree branches with a diameter of 1 cm, after drying, cut the branches into 3 cm long short sticks with a cutter; put the short sticks into 700 mL plastic spawn bottles, so that the height of the material surface from the mouth of the bottle is 4cm; then fill 380mL nutrient solution into the plastic seed bottle, cover the bottle cap, and sterilize at 0.1MPa, 120°C for 2 hours; then cool the plastic seed bottle to room temperature and place it on the inoculation line Above, perform aseptic inoculation, the inoculation volume of each bottle is 18g of the original seed; then put the plastic seed bottle in the cultivation room, and cultivate it in the dark at 22°C, and conduct mycelium germination and pollution investigation after 6 days of inoculation, and discharge Strains that cannot germinate and are contaminated; when the strains eat 1 / 3 of the material, observe the growth of mycelia and discharge impurities to exclude the strains that do not eat the fee...

Embodiment 2

[0030] Gather birch tree branches with a diameter of 3cm, and after drying, cut the branches into short sticks with a length of 5cm; put the short sticks into 800mL plastic spawn bottles so that the material surface is at the height of the bottle mouth 2cm; then fill 420mL nutrient solution into the plastic seed bottle, cover the bottle cap, and sterilize at 0.15MPa, 121°C for 2.2h; then cool the plastic seed bottle to room temperature and place On the inoculation line, carry out aseptic inoculation, and the inoculation volume of each bottle is 22g of the original seed; then put the plastic seed bottle in the cultivation room, and cultivate it in the dark at a temperature of 25°C, and conduct mycelium germination and pollution investigation after 8 days of inoculation , discharge the strains that cannot germinate and are contaminated; when the strains eat 1 / 3 of the material, observe the growth of the mycelia and discharge impurities, and exclude the strains that do not eat the...

Embodiment 3

[0034] Gather the branch that the diameter of wild cherry tree is 2cm, after drying, this branch is cut into the short stick of 4cm with cutting machine; This short stick is packed in the plastic spawn bottle of 750mL, makes material surface be apart from the height of bottle mouth 3cm; then fill 380mL nutrient solution into the plastic seed bottle, cover the bottle cap, and sterilize at 0.1MPa, 121°C for 2.5h; then cool the plastic seed bottle to room temperature and place On the inoculation line, carry out aseptic inoculation, and the inoculation volume of each bottle is 20g of the original seed; then put the plastic seed bottle in the cultivation room, and cultivate it in the dark at a temperature of 25°C, and conduct mycelium germination and pollution investigation after 7 days of inoculation , discharge the strains that cannot germinate and are contaminated; when the strains eat 1 / 3 of the material, observe the growth of the mycelia and discharge impurities, and exclude th...

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Abstract

The invention discloses a production method of a gastrodia elata associated honey fungus strain. The method comprises the following steps: selecting branches of a broad-leaved tree, cutting the branches into cylindrical short rods, and putting the short rods in a bottle; filling the bottle with a nutrient solution and covering the bottle with a bottle cap to conduct sterilization treatment; cooling the bottle to ordinary temperature, and then conducting inoculation; conducting cultivation at a certain temperature in a dark place. According to the method, the branches are taken as a culture medium, and the nutrient solution is added to provide sufficient nutrition for the honey fungus strain and shorten the production period of the strain. Meanwhile, by checking the germination, growth condition and contamination of mycelia at reasonable time, the strains that don't germinate and eat, grow bad and are contaminated are removed, so that the quality of the strain is effectively improved, the activity and momentum of the strain are ensured, and a high-quality strain is provided for gastrodia elata planting.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a preparation method of an Armillaria strain associated with Gastrodia elata. Background technique [0002] Tianma, also known as Chijian, Duyaozhi, Limu, Helicao, Shencao, Guiduyou, Mupu, Mingma, Dingfengcao, Bailongpi, etc., is a perennial herb of the genus Gastrodia in the family Orchidaceae. The rhizome is thick, without green leaves, and the capsule is obovate-elliptic, often propagated by tubers or seeds. Its rhizome is used medicinally to treat dizziness, numbness and other diseases. Because Gastrodia elata has a wide range of applications and a large market demand, Gastrodia elata is planted in large quantities in various places. Before planting Gastrodia elata, it is necessary to cultivate the Armillaria fungus material or fungus bed. [0003] Armillaria belongs to the class Basidiomycetes, Agaricaceae, a genus of fungi, and the fruiting bodies are generally medium-s...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00C05B7/00C05G3/00C05D5/00C05F11/00C05G5/23
Inventor 龚光禄肖永东
Owner 关岭自治县丰硕食用菌农民专业合作社
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