Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting biological molecules based on upconversion luminescent material and detection system implementing same

A technology for luminescent substances and biomolecules, applied in the field of high-sensitivity quantitative or qualitative detection, to achieve high signal-to-noise ratio, eliminate interference, and improve signal-to-noise ratio

Active Publication Date: 2015-02-11
杨晓林 +2
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the fact that the scattering and absorption of excitation light and emission light have not been completely solved, as well as excitation saturation and concentration quenching in solid-phase detection, the main technical indicators such as sensitivity, accuracy and linear range are still unable to truly meet high-precision quantitative detection. Therefore, it can only be applied to qualitative or semi-quantitative detection methods such as immunochromatography

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting biological molecules based on upconversion luminescent material and detection system implementing same
  • Method for detecting biological molecules based on upconversion luminescent material and detection system implementing same
  • Method for detecting biological molecules based on upconversion luminescent material and detection system implementing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Utilizing the up-conversion luminescent label emitting visible light of the present invention Quantitative Detection of Thyrotropin (Protein) in Human Serum by Immunological Sandwich Assay.

[0056] 1. Select two anti-human thyroid-stimulating hormone (TSH) monoclonal antibodies (purchased from Finland Medix biochemical company) that do not compete with each other.

[0057]One strain is used to coat magnetic particles (polystyrene-coated magnetic particles are purchased from Merck, Germany), coating conditions: 20 μg / mL antibody is added to 20 mM / L pH9.6 carbonate buffer, and 1% (volume weight ratio) is added Shake the magnetic particles overnight at 4°C; discard the liquid, add the same volume of 1% (volume to weight ratio) casein, shake at room temperature for 2 hours, and discard the liquid for later use.

[0058] Another strain with up-conversion luminescent substance—trans-4-(dimethylamino)-4`-(diethylamino)stilbene (DMAEAS) nanoparticles (according to Yan Yunxin...

Embodiment 2

[0074] The ultraviolet-emitting up-conversion luminescent label immunocompetitive analysis method of the present invention is used to quantitatively detect human serum thyroxine (small molecule hormone and drug).

[0075] 1. Thyroxine (T 4 ) (produced by Sigma, USA) covalently bonded with casein to form a complex: the EDC method was used, and EDC was purchased from Thermo Fisher, USA, and operated according to the product instructions. Then use the complex to coat the microwell plate, coating conditions: add 0.5μg / mL complex to 20mM / L pH9.6 carbonic acid buffer, add 100μl to each well, overnight at 4°C; discard the liquid, add the same volume of 1% (Volume to weight ratio) The casein was shaken at room temperature for 2 hours, and the liquid was discarded for later use.

[0076] Up-converting luminescent substance (Y 2 o 3 :Yb 3+ ,Tm 3+ ) nanoparticles (according to Li Tanggang et al., Y 2 o 3 :Yb 3+ , Tm 3+ Visible and ultraviolet up-conversion luminescence of nanoma...

Embodiment 3

[0088] The ultraviolet-emitting up-conversion luminescence-labeled sandwich oligonucleotide molecular hybridization method of the present invention is used to qualitatively detect human sputum Mycobacterium tuberculosis 16s RNA (nucleic acid fragment).

[0089] 1. Artificially synthesized two human tuberculosis 16sRNA-specific oligonucleotide probes 5`-GGT GGA AAG CGC TTT AGC GGT-3`-Biotin and Dig-5` labeled with biotin and digoxin respectively -GCC CGT ATC GCC CGC ACG CTC ACA-3` (synthesized by American Sybersyn Company).

[0090] 2. Biotin (produced by Sigma, USA) to coat the microwell plate, coating conditions: add 20 μg / mL biotin to 20 mM / L pH9.6 carbonic acid buffer, add 100 μl to each well, overnight at 4°C; discard the liquid, add The same volume of 1% (volume to weight ratio) casein was shaken at room temperature for 2 hours, and the liquid was discarded for later use.

[0091] Up-converting luminescent substance (Y 2 o 3 :Yb 3+ ,Tm 3+ ) nanoparticles (according t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for detecting biological molecules based on an upconversion luminescent material. The method comprises the following steps: 1) combining the to-be-detected biological molecules with markers, wherein the markers are prepared by using the upconversion luminescent material; and then removing uncombined excess markers; 2) adding a dissociation agent into the reaction system of the step 1), and making the upconversion luminescent material dissociated to a liquid phase; and 3) detecting the presence or the amount of the markers or the upconversion luminescent material in the reaction system of the step 2). The method is a multi-photon excitation upconversion luminescent mark liquid phase detection technology suitable for quantitative or qualitative detection of the biological molecules. The invention also provides a detection system for implementing the method.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to high-sensitivity quantitative or qualitative detection of biomolecules. Background technique [0002] As an important part of modern biotechnology, biomolecular marker detection technology represented by immunological detection and gene analysis has not only been widely used in the field of biomedical research, but also has been widely used in clinical diagnosis, environmental sanitation detection, food safety, etc. It has become a necessary conventional technical means in many departments such as medical examination and forensic identification, and plays an irreplaceable role. As the key markers and their corresponding signal detection methods, they have gone through the following major stages of technological development so far: [0003] 1. Radioactive labeling: It appeared in the middle of the last century and laid the foundation for the development of mode...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63C12Q1/68C12M1/42
Inventor 杨晓林孙旭东刘红
Owner 杨晓林
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com