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Composite oligopeptide with high oxidation activity inhibition and preparation method thereof

A technology of oxidative activity and anti-oxidative peptides, which is applied in the field of food to achieve the effects of no toxic side effects, rich nutrition, and simple preparation methods

Inactive Publication Date: 2015-02-18
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is a lack of a compound oligopeptide with high antioxidant activity and a preparation method thereof

Method used

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  • Composite oligopeptide with high oxidation activity inhibition and preparation method thereof
  • Composite oligopeptide with high oxidation activity inhibition and preparation method thereof
  • Composite oligopeptide with high oxidation activity inhibition and preparation method thereof

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Embodiment 1

[0033] The preparation of proteolysis solution of the present invention, single enzyme and double enzyme enzymatic hydrolysis conditions step by step are as shown in Table 1,

[0034] Table 1

[0035]

[0036]

[0037] Ⅰ means egg white protein, Ⅱ means soybean protein isolate, P means pepsin, A means alkaline protease, ⅠPH means pepsin hydrolyzate of egg white protein, and so on.

[0038] The preparation method of a kind of compound oligopeptide with high antioxidant activity provided by the invention comprises the following steps:

[0039] (1) Using pepsin and alkaline protease to hydrolyze egg white protein and soybean protein respectively with single enzyme or double enzymes in sequence, taking one of the above-mentioned egg white protein hydrolyzate and one of the above-mentioned soybean protein hydrolyzate;

[0040] (2) Use ultrafiltration and resin to enrich the strong hydrophobic antioxidant peptides in the hydrolyzate of the above two protein sources, and obtai...

Embodiment 2

[0047] In step (1), use egg white protein powder and soybean protein powder as raw materials, add water to prepare a protein suspension, keep at 85°C-95°C for 10 minutes, and keep stirring to denature the protein, cool to room temperature, and add alkali Protease for hydrolysis; after the end of enzymolysis, add pepsin for hydrolysis, inactivate the enzyme after reaction, adjust the pH value to 5.5 after cooling to room temperature, centrifuge and collect the supernatant; the mass concentration of egg white protein or soybean protein suspension is 1%-6%. The alkaline protease enzymatic hydrolysis conditions are as follows: the amount of enzyme added is 8000U / 1g protein; the pH value is 9.0; the hydrolysis temperature is 60°C, and the enzymatic hydrolysis time is 5h. The pepsin hydrolysis conditions are as follows: the amount of enzyme added is 15000U / 1g protein; the pH value is 2.0; the temperature is 38°C, and the hydrolysis time is 5h. The hydrolysis sequence of pepsin and ...

Embodiment 3

[0051] The preparation method of a kind of compound oligopeptide with high antioxidant activity provided by the invention comprises the following steps:

[0052] In step (1), use egg white protein powder and soybean protein powder as raw materials, add water to prepare protein suspension, keep at 95°C for 10 minutes, and keep stirring to denature the protein, cool to room temperature, add alkaline protease Hydrolysis; after enzymatic hydrolysis, add pepsin for hydrolysis, inactivate the enzyme after reaction, adjust the pH value to 6.0 after cooling to room temperature, centrifuge and collect the supernatant; the mass concentration of egg white protein or soybean protein suspension is 6%. The alkaline protease enzymatic hydrolysis conditions are as follows: the amount of enzyme added is 12000U / 1g protein; the pH value is 11.0; the hydrolysis temperature is 70°C, and the enzymatic hydrolysis time is 6h. The pepsin hydrolysis conditions were as follows: the amount of enzyme adde...

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Abstract

The invention discloses a composite oligopeptide with high oxidation activity inhibition and a preparation method thereof. The preparation method of the composite oligopeptide with high oxidation activity inhibition comprises the following steps: (1) respectively hydrolyzing egg white protein and soybean protein by using a single enzyme or double enzymes of pepsin and alkaline protease, and obtaining one of the egg white protein hydrolysate and the soybean protein hydrolysate; (2) enriching high-hydrophobicity antioxidative peptide in the two protein hydrolysate sources by using resin in an ultra-filtration manner, and obtaining a target peptide, namely a rough resin separation component, by virtue of gradient elution; and (3) respectively compounding every two of the protein hydrolysate sources obtained in the step (1) or / and two protein hydrolysate sources obtained in the step (2), thereby obtaining the composite oligopeptide. The composite oligopeptide disclosed by the invention is high in oxidation activity inhibition, and the preparation method is simple.

Description

technical field [0001] The invention relates to the field of food technology, in particular to a composite oligopeptide with high antioxidant activity and a preparation method thereof. Background technique [0002] Antioxidant peptides produced by enzymatic hydrolysis of animal and plant proteins, marine proteins, and waste protein resources have the characteristics of small molecular weight, easy absorption, strong activity, and high safety, and can effectively remove excess active oxygen free radicals in the body to protect cells and The normal structure and function of mitochondria can prevent the occurrence of lipid peroxidation, and it has broad application prospects in medicine, cosmetics, health products, food and feed additives, etc. Soybean protein and egg protein have high biological potency and nutritional value, and the physiologically active soybean peptide and egg peptide prepared after hydrolysis have high biological potency and nutritional value. There are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
Inventor 饶胜其方维明黄阿根高璐杨振泉尹永祺
Owner YANGZHOU UNIV
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