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Microecological preparation suitable for marine culture and application thereof

A micro-ecological preparation and mariculture technology, applied in the direction of microorganism-based methods, microorganisms, medical preparations containing active ingredients, etc., can solve the problem of fewer probiotic strains, so as to promote growth, increase activity, and enhance cellular immunity and humoral immune function

Active Publication Date: 2015-03-04
XUZHOU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are still few confirmed strains of probiotics suitable for production

Method used

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  • Microecological preparation suitable for marine culture and application thereof
  • Microecological preparation suitable for marine culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the cultivation of probiotic strain

[0042]The probiotic strains used in the present invention are Bacillus subtilis (Bacilus sbutilis), Bifidobacterium bifidum (Bifidobacterium bifidum), Lactobacillus acidophilus (Lactobacillus acidophilus), photosynthetic bacteria (Rhodobacter sphaeroides), according to the medium recommended by the strain preservation agency And culture conditions and culture 12 ~ 48h to the required amount of bacteria. in:

[0043] Bacilus sbutilis, such as ATCC6633 or CGMCC 4018; Bifidobacterium bifidum, such as CICC6170 or CCTCC M203049; Lactobacillus acidophilus, such as CICC 6088 or CGMCC1.1854; Photosynthetic bacteria (Rhodobacter sphaeroides), such as: CGMCC8248.

Embodiment 2

[0044] Example 2: Preparation and survival detection of probiotics

[0045] The number of viable bacteria in each bacterial culture solution in Example 1 was measured by the plate counting method, and the compound microecological preparation was prepared according to a certain biomass ratio according to the number of viable bacteria of each bacteria. Among them, the number of live bacteria of Bacillus subtilis contained in each gram of preparation is (1.0~5.0)×10 9 cfu, the number of viable bifidobacteria is (1.0~5.0)×10 9 cfu, the number of viable Lactobacillus acidophilus is (3.0~5.0)×10 9 cfu, the number of viable photosynthetic bacteria is (1.0~5.0)×10 9 cfu. In terms of mass percentage, the amount of Bacillus subtilis is 0.5-1.0% (w / w), the amount of bifidobacteria is 0.1-0.5% (w / w), and the amount of Lactobacillus acidophilus is 1.0-3.0% (w / w) , the dosage of photosynthetic bacteria is 0.5-1.0% (w / w), and the balance is water.

[0046] Preferably, in every gram of c...

Embodiment 3

[0048] Embodiment 3: Inhibition to aquaculture pathogenic bacteria

[0049] Five common aquatic pathogenic bacteria, Vibrio parahaemolyticus, Vibrio resplendent, Vibrio anguillarum, Pseudomonas fluorescens, and Aeromonas hydrophila, were added to appropriate sterile media and cultivated to logarithmic production Expect. The different proportions of probiotics prepared in Example 2 were respectively spotted on the surface of the solid medium containing pathogenic bacteria, and cultivated overnight. On the next day, test its ability to inhibit pathogenic bacteria by observing whether a bacteriostatic zone is produced, and the results are as follows: figure 1 It was shown that the prepared probiotics had a good inhibitory effect on the five strains of aquatic pathogenic bacteria tested. Among them, the biomass ratio is preferably 1:0.8:0.8:1.2.

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Abstract

The invention relates to the field of marine culture, and particularly relates to a microecological preparation suitable for the marine culture and application thereof. The microecological preparation is a compound microecological preparation which is compounded of bacillus subtilis, bifidobacterium, lactobacillus acidophilus and photosynthetic bacteria according to viable bacterium biomass. According to the invention, by optimally compounding multiple probiotics, a microbial inoculum compounding mode capable of effectively inhibiting the growth of aquatic harmful bacteria and reducing the influence of wastes such as residual baits and excrements of cultured species on a culture environment in a culture process can be screened out under the precondition that the growth properties of cultured species are not reduced, thus providing the scientific basis for reducing the occurrence rate of aquatic diseases by utilizing the probiotics and reducing the environmental pollution brought by a culture process. An experiment result shows that the compound microecological preparation can be used for inhibiting the common aquatic pathogenic bacteria and effectively reducing the content of ammonia nitrogen, nitrite nitrogen and total phosphorus which are contained in the culture wastes.

Description

technical field [0001] The invention relates to the field of mariculture, in particular to a microecological preparation suitable for mariculture and its application. Background technique [0002] In the aquaculture industry, with the increasing promotion of intensive and high-density factory farming models, disease problems are becoming more and more serious. In the actual breeding process, antibiotics are usually used for the prevention and control of microbial diseases. Due to the risk of antibiotic accumulation or transmission along the food chain, the safety of farmed products is greatly affected. Since the end of the 1990s, major developed countries such as Europe and Japan have taken official tough measures to strictly limit the types, methods of use, doses and compatibility of antibiotics used in feed. Since 2002, the European Union has banned the addition of antibiotic products in feed to promote growth. my country has also issued a series of laws and regulations ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61K35/741A61P31/04C02F3/34C12R1/01C12R1/125
CPCA61K35/74A61K35/742A61K35/745A61K35/747C02F3/341C02F2103/20C12N1/20A61K2300/00
Inventor 焦绪栋侯进慧刘全德
Owner XUZHOU UNIV OF TECH
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