Method for improving germination rate of Puccinellia tenuiflora seed

A technology of S. florida and germination rate, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the recovery of S. florida vegetation, low germination potential and germination rate, no emergence of seedlings, etc., to achieve Improve the germination potential and germination rate, non-pathogenicity and stress resistance, and the effect of not polluting the environment

Inactive Publication Date: 2015-03-11
LANZHOU UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, compared with the seeds of other gramineous crops, the seeds of S. florida are thin and small, and their germination potential and germination rate are low. After sowing, there will be no emergence or late emergence, which seriously restricts the improvement of S. florida in saline-alkali land and vegetation recovery. role in

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  • Method for improving germination rate of Puccinellia tenuiflora seed
  • Method for improving germination rate of Puccinellia tenuiflora seed
  • Method for improving germination rate of Puccinellia tenuiflora seed

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Effect test

Embodiment 1

[0020] Disinfection treatment: select the seeds of Echinacea florida with full grains, no defects, and uniformity, soak them in 75% alcohol and 5% sodium hypochlorite for 10 seconds and 10 minutes respectively on the ultra-clean workbench, then rinse them with sterilized distilled water for 7 to 8 times. Stir continuously during the washing process to rinse the alcohol and sodium hypochlorite on the surface of the seeds.

[0021] Soaking treatment: Add sterile water into a sterilized centrifuge tube, then soak the sterilized A. parsley seeds in sterile water. After timing for 5 minutes, quickly take out the seeds and spread them on a sterilized container covered with two layers of filter paper. In the petri dish, before planting seeds, drip the same amount of sterile water on the filter paper until a water film is formed on the surface of the filter paper, spread the taken out seeds evenly on the filter paper, 100 seeds per dish, and culture in the dark at 25°C , Statistically...

Embodiment 2

[0024] Disinfection treatment: select the seeds of Echinacea florida with full grains, no defects, and uniformity, soak them in 75% alcohol and 5% sodium hypochlorite for 10 seconds and 10 minutes respectively on the ultra-clean workbench, then rinse them with sterilized distilled water for 7 to 8 times. Stir continuously during the washing process to rinse the alcohol and sodium hypochlorite on the surface of the seeds.

[0025] Preparation of lysate broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, sterilize at a high temperature of 115-132°C, and then cool to 20 ~35°C.

[0026] Soaking treatment: Add LB liquid culture medium into the sterilized centrifuge tube, then soak the sterilized S. florida seeds in the LB liquid culture medium. In a petri dish with one layer of filter paper, before sowing, drip an equal amount of sterile water on the filter paper until ...

Embodiment 3

[0029] Disinfection treatment: select the seeds of Echinacea florida with full grains, no defects, and uniformity, soak them in 75% alcohol and 5% sodium hypochlorite for 10 seconds and 10 minutes respectively on the ultra-clean workbench, then rinse them with sterilized distilled water for 7 to 8 times. Stir continuously during the washing process to rinse the alcohol and sodium hypochlorite on the surface of the seeds.

[0030] Preparation of Bacillus subtilis GB03 bacterial liquid: first prepare the lysis broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, and the high temperature is 115~ Sterilize at 132°C, then cool to 20-35°C, add 50-100 μL of Bacillus subtilis GB03 bacterial liquid per 100 mL of LB liquid medium, at a speed of 180-250 rpm and a temperature of 25-30°C After culturing overnight on a constant temperature culture shaker, the concentration of the ...

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Abstract

The invention relates to a method for improving germination rate of Puccinellia tenuiflora seeds. Before germination, the Puccinellia tenuiflora seeds are soaked in a Bacillus subtilis GB03 bacteria liquid. The scheme for treating Puccinellia tenuiflora seeds can effectively improve the germination potential and germination rate of Puccinellia tenuiflora seeds, alleviate the phenomena of no seedling emergence or late seedling emergence, irregular seedling emergence, seedling lack and ridge break after sowing, and ensure the role of pioneer species of Puccinellia tenuiflora in the improvement of saline alkali soil and vegetation restoration. At the same time, Bacillus subtilis the characteristics of no toxicity or harm to human and animal, no pollution to the environment, no pathogenicity and strong resistance. The method for improving the germination rate of Puccinellia tenuiflora seeds provided by the invention has simple operation, can be widely applied to planting of pasture forage, and has important economic value.

Description

technical field [0001] The invention relates to the field of forest and grass planting, in particular to a method for improving the germination rate of Echinopsis florida seeds. Background technique [0002] S. florida, also known as star grass, is a typical salt-repelling halophyte in Gramineae. It has a wide range of distribution in the saline-alkali land in Northeast and Northwest my country, and is a dominant species and a constructive species in saline meadows. S. florida can not only be used as a pioneer species for vegetation restoration in saline-alkali land, but also has strong drought and cold tolerance, and has a close relationship with the grass crop wheat. Therefore, S. florida can be used as a model plant for studying salt tolerance of crops . In recent years, with the rapid development of grassland agriculture in the country, S. florida, as a high-quality salt-tolerant pasture grass, has achieved initial results in the practice of saline-alkali land improveme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张金林李静韩庆庆吕昕培葛静李惠茹王锁民
Owner LANZHOU UNIVERSITY
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