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Extracting method and application of high-purity coptisine

An extraction method, the technology of coptisine, applied in the field of biological extraction, can solve the problems of poor safety of chloroform recrystallization, difficulty in meeting large-scale preparation, unfavorable industrial production, etc., and achieve low product cost, low cost, and improved production efficiency Effect

Active Publication Date: 2015-03-18
重庆伊士腾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Master's degree thesis of Sichuan University, 2007); this process technology requires the use of expensive reagent potassium iodide, and at the same time needs to be separated on the column, the process is cumbersome; and the safety of chloroform recrystallization is not good, it is difficult to meet the needs of large-scale preparation
Chinese herbal medicine, 2006, 37 (7): 1017); due to the use of preparative liquid chromatography, the equipment is expensive and the investment is large, which is also not conducive to the realization of industrial production
[0013] In summary, although there are some reports on the process technology for extracting coptisine, either the process technology is complicated, or expensive reagents are needed, or toxic raw materials are involved.

Method used

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  • Extracting method and application of high-purity coptisine
  • Extracting method and application of high-purity coptisine
  • Extracting method and application of high-purity coptisine

Examples

Experimental program
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Effect test

Embodiment 1

[0063] 1 kg of Coptidis Rhizome is crushed or sliced, added with 3 liters of 5% sulfuric acid solution, soaked for 1 hour, heated to 100°C for reflux extraction for 0.1 hour, filtered; the residue is extracted four times with 3 liters of 5% sulfuric acid solution raised to 100°C; Combine the first and second extracts (three extractions and four extractions are used for the processing of the next batch of raw materials), and separate the supernatant after cooling (or filter to obtain the supernatant); the supernatant is made of lime (oxidized Calcium) neutralized to pH=2, filtered, the precipitate was washed 3 times with 0.1 times the precipitation amount of water; combined the filtrate and washing liquid, added 10% concentrated hydrochloric acid, placed at low temperature (0°C) for 100 hours, filtered to remove impurities, and precipitated Wash with 0.1 times the volume of the precipitated water; combine the filtrate and washing liquid, add 0.1% concentrated sulfuric acid (V / V)...

Embodiment 2

[0065] Grind or slice 1 kg of Coptis chinensis, add 15 liters of 0.1% sulfuric acid solution, soak for 48 hours, heat up to 20°C and extract for 5 hours, filter; then use 15 liters of 0.1% sulfuric acid solution for heat preservation and extraction four times; combine the first The first and second extractions (three extractions and four extractions are used for the processing of the next batch of raw materials), and the supernatant is separated after cooling (or filtered to obtain the supernatant); the supernatant is used in lime (calcium oxide) and to pH=12, filter, and wash the precipitate once with 3 times the precipitation amount of water; combine the filtrate and washing liquid, and concentrate 20 times under reduced pressure; add 0.1% concentrated hydrochloric acid and 10% sodium chloride, ) to stand for 1 hour, filter to remove impurities, and wash the precipitate with 1 times the volume of the precipitate; combine the filtrate and washing liquid, add 10% concentrated s...

Embodiment 3

[0067] Grind or slice 1 kg of Coptis chinensis, add 8 liters of 3% sulfuric acid solution, soak for 12 hours, heat up to 70°C and extract for 2 hours, and filter; the residue is extracted four times with 8 liters of 3% sulfuric acid solution; combine the first The first and second extractions (three extractions and four extractions are used for the processing of the next batch of raw materials), and the supernatant is separated after cooling (or filtered to obtain the supernatant); the supernatant is used in lime (calcium oxide) and to pH=6, filter, and wash the precipitate twice with water of 1 times the amount of precipitation; combine the filtrate and washing liquid, concentrate under reduced pressure 6 times; add 5% concentrated hydrochloric acid and 5% sodium chloride, ) for 24 hours, filtered to remove impurities, and the precipitate was washed with 0.5 times the volume of the precipitated water; the filtrate and washing liquid were combined, 2% concentrated sulfuric acid...

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Abstract

The invention relates to an extracting method and an application of high-purity coptisine. The extracting method comprises the following technological processes: taking raw materials (rhizoma coptidis or rhizoma coptidis fibrous roots or rhizoma coptidis ash), crushing the raw materials (if the raw materials are the rhizoma coptidis fibrous roots or rhizoma coptidis ash, a crushing process is not needed), adding 0.1-5% of sulfuric acid for soaking, performing heating extraction for four times, filtering, performing neutralization by virtue of calcium oxide (or calcium hydroxide), filtering and washing precipitates, performing preconcentration, removing impurities from the precipitates, depositing coptisine, performing re-crystallization on coptisine, and drying the precipitates to obtain a coptisine product with the rhizoma coptidis content of more than 90%. The product disclosed by the invention has good effects of reducing blood fat, losing weight, reducing blood sugar and the like, and is the ideal raw material for treating cardiovascular diseases, adiposis and diabetes.

Description

technical field [0001] The invention belongs to biological extraction technology, in particular to a high-purity coptisine extraction technology. Background technique [0002] Coptis chinensis was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Coptis chinensis is bitter in taste, cold in nature, and has the functions of purging fire, detoxifying, clearing away heat, and drying dampness. For dysphoria, confusion, upset and insomnia, hot and humid fullness, vomiting, abdominal pain and diarrhea, conjunctival congestion and swelling, sore tongue, eczema, burns, vomiting blood, epistaxis, etc. The results of modern pharmaceutical research show that the main active ingredients of Coptis chinensis are Coptis chinensis alkaloids, mainly including berberine (berberine), palmatine, coptisine and epiberberine ("Chinese Pharmacopoeia" 2010 edition); It has a wide range of pharmacological activities such as lowering blood fat and blood sugar; recent studies ha...

Claims

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Application Information

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IPC IPC(8): C07D455/03A61K31/4375A61P3/06A61P3/04
CPCA61K31/4375C07D455/03
Inventor 叶小利李学刚
Owner 重庆伊士腾生物科技有限公司
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