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Natural plant extract and application thereof of preventing and treating nosema apis

A natural plant and extract technology, applied in plant raw materials, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve problems such as side effects and drug residues, achieve efficient prevention and treatment, prolong life, good effect

Active Publication Date: 2015-03-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But as an antibiotic, long-term use of fumagillin in bee colonies can lead to drug residue problems in bee products
A large number of studies have shown that fumagillin has side effects in the treatment of human encephalitic microsporidiosis and cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Weigh 10 g of grape skins dried in an oven at 37 °C, add 250 ml of 75% ethanol solution, leave it sealed at room temperature for 4 h, ultrasonically extract at 45 °C for 60 min, and filter to obtain the filtrate. Then add 100 ml of 75% ethanol solution to the filtered residue, repeat the above ultrasonic steps, take the filtrate, and combine the two filtrates. Ethanol was evaporated to dryness in a rotary evaporator at 55°C until it became a paste liquid. Take a certain amount of grape skin extract extract and 70% sucrose syrup to prepare a grape skin extract syrup mixture with a mass volume ratio of 3%, allowing the bees infected with Microsporidia to feed freely.

[0011] Results: On the 7th day and 13th day after infection, the number of microsporidian spores in the bees in the experimental group was 29.9% and 32.9% of those in the untreated group, respectively. On the 13th day, the survival rate of the bees in the experimental group was 87%. The survival rate of be...

Embodiment 2

[0013] Weigh 10 g of grape skins dried in an oven at 37°C, add 250 ml of 75% ethanol solution, place it sealed at room temperature for 4 hours, extract ultrasonically at 45°C for 60 minutes, and filter to obtain the filtrate. Then add 100 ml of 75% ethanol solution to the filtered residue, repeat the above ultrasonic steps, take the filtrate, and combine the two filtrates. Ethanol was evaporated to dryness in a rotary evaporator at 55°C until it became a paste liquid. Take a certain amount of grape skin extract extract and 70% sucrose syrup to prepare a grape skin extract syrup mixture with a mass volume ratio of 1%, allowing the bees infected with Microsporidia to feed freely.

[0014] Results: On the 7th day and 13th day after infection, the number of Microsporidia spores in the experimental group was 23.5% and 48.2% of that in the untreated group, respectively. On the 13th day, the survival rate of bees in the experimental group was 90%, while the survival rate of bees in ...

Embodiment 3

[0016] Weigh 10 g of grape skins dried in an oven at 37°C, add 250 ml of 75% ethanol solution, place it sealed at room temperature for 4 hours, extract ultrasonically at 45°C for 60 minutes, and filter to obtain the filtrate. Then add 100 ml of 75% ethanol solution to the filtered residue, repeat the above ultrasonic steps, take the filtrate, and combine the two filtrates. Ethanol was evaporated to dryness in a rotary evaporator at 55°C until it became a paste liquid. Take a certain amount of grape skin extract extract and 70% sucrose syrup to prepare a grape skin extract syrup mixture with a mass volume ratio of 0.5%, allowing the bees infected with Microsporidia to feed freely.

[0017] Results: On the 7th day and 13th day after infection, the number of Microsporidia spores in the experimental group was 34.2% and 65.9% of that in the untreated group, respectively. On the 13th day, the survival rate of bees in the experimental group was 93%, while the survival rate of bees i...

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Abstract

The invention provides a natural plant extract. The natural plant extract is applied to prepare a drug of preventing and treating nosema apis as follows: by taking grape skin as a raw material, leaching by an alcohol solvent, and extracting a pasty substance by virtue of processes such as ultrasonic crushing and rotary evaporation; then, preparing the pastry substance into a grape skin extract syrup mixture in certain proportion, so as to prepare the drug of preventing and treating nosema apis. During use, a feeder is filled with the natural plant extract for enabling bees infected with the microsporidia to freely take food, and acted to the bees and the microsporidia, so that the effects of effectively preventing and treating the nosema apis are achieved. The natural plant extract has no toxic or side effects on bees and is rich in flavonoid compounds such as anthocyanin as well as substances such as tannin and cellulose. The control effect on the nosema apis is good, the raw material source of the drug is wide, easily available, simple to prepare and low in cost. Moreover, the natural plant is free of pollution on bee products and environment, and is a healthy, environment-friendly, ecological and efficient new way of preventing and treating nosema apis.

Description

technical field [0001] The invention belongs to the field of bee feeding and management, and relates to a drug for preventing and controlling bee diseases, in particular to a natural plant extract for preventing and treating bee microsporidiosis and its medicinal application. Background technique [0002] Honey bee microsporidiosis, also known as "bee microsporidiosis", is a common digestive tract infectious disease of adult bees caused by honey bee microsporidiosis. Microsporidium is a primitive eukaryotic parasite specializing in intracellular parasites. Microsporidium honeybee mainly destroys the normal digestive function of honeybee intestinal tract by infecting adult bee intestinal epithelial cells. When the bee colony is mildly ill, the symptoms are not obvious and are often ignored, but once it is severe, the digestive ability of the bees will be reduced, immunity and collection ability will be reduced, the life span of the bees will be shortened, the colony of the be...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61P33/02
CPCA61K36/87A61K2236/333A61K2236/39A61K2236/51Y02A50/30
Inventor 郑火青陈秀贤胡福良贡红日
Owner ZHEJIANG UNIV
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