A group of amino acid sequences that can improve the heat-resistant temperature and thermostability of SOD and its application

A technology of heat-resistant temperature and thermal stability, applied in the fields of biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problems of less obvious effect, difficult to meet the needs of industrialization, difficult to operate, etc.
CN104450632BActive Publication Date: 2017-04-19北京迈恩泰生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京迈恩泰生物科技有限公司
Publication Date
2017-04-19

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Abstract

The invention relates to an amino acid sequence capable of improving heat-resistance temperature and heat stability of SOD and application thereof. The amino acid sequence is an N-terminal amino acid sequence of 13 special thermophilic Fe / Mn-SOD from Geobacillus category. The amino acid sequence has the following features: (1) the amino acid sequence is before the sodA functional domain of Fe / Mn-SOD protein, the sequence length is 101-344 amino acids and the function is unknown; (2) 85-100% of homology exists among the amino acid sequence; (3) one or two peculiar consensus repeats Geo-N-repeat are included. The amino acid sequence can play a determinant role on the heat-resistance temperature and the heat stability of Fe / Mn-SOD of the Geobacillus category, can be widely applied to improvement on other SODs (particularly normal-temperature SOD), and can remarkably improve the heat-resistance temperature and the heat stability.
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Description

technical field

[0001] The invention relates to a group of amino acid sequences capable of improving the heat-resistant temperature and thermal stability of SOD and its application. Background technique

[0002] Superoxide dismutase (SOD) is the only enzyme that can specifically scavenge free radical O 2 · ˉ The antioxidant metalloenzyme is the key to the defense of organisms against oxygen toxicity. The enzyme widely exists in organisms in nature. Since it was discovered and officially named from bovine red blood cells in 1969, scientists have isolated it from bacteria, fungi, protozoa, algae, insects, fish, plants and mammals. SOD. Due to the special effects of SOD, it has wide application value in the fields of medicine, daily chemical industry, food, agriculture and environmental protection. At present, the clinical application of SOD is mainly focused on anti-inflammation (mainly rheumatoid and inflammation patients caused by radiation therapy), in addition to some ...

Claims

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