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Method for preparing chondroitin sulfate and co-producing collagen peptide and biological calcium by using fishbone

A technology of chondroitin sulfate and collagen peptide, which is applied in the direction of calcium carbonate/strontium/barium, animal/human protein, specific peptides, etc., can solve the problems of chondroitin sulfate destruction, water environment pollution, large ethanol consumption, etc., and achieve Promote dissolution, reduce production costs, and achieve stable and reliable quality

Inactive Publication Date: 2015-04-01
RONGCHENG HONGYE OCEAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most used method is to extract chondroitin sulfate by combining dilute alkali or concentrated alkali with enzymatic hydrolysis. This alkaline enzymatic hydrolysis production process has cumbersome steps and a long cycle. In the process of decolorization and decolorization, chondroitin sulfate is severely damaged, the product yield is low, the consumption of ethanol is large, and the production cost is high
At present, a large amount of animal protein and its degradation products produced in the production process of chondroitin sulfate are discharged without utilization, causing serious pollution to the water environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method for preparing chondroitin sulfate from fish bones and co-producing collagen peptides and biological calcium is through the following specific process steps:

[0028] (1) Cooking

[0029] Select fresh marine fish bones as raw materials, send them to a continuous cooking tank for cooking for 30 minutes, and separate them with high-pressure air to obtain fish bone protein and fish bones;

[0030] (2) Crushing and fermentation

[0031] Wash and crush the fish bones obtained in step (1), and control the average particle size to 0.3 mm to obtain fish bone powder; add the obtained fish bone powder to an equal amount of pure water and stir evenly, and then inoculate the fish bone powder with a mass of 2% lactic acid bacteria, adjust the pH value to 5.5, ferment at a constant temperature, control the temperature at 37°C, and ferment for 18 hours to obtain a fermented liquid;

[0032] (3) Separation

[0033] centrifuging the fermented liquid obtained in step (2) to ...

Embodiment 2

[0044] The method for preparing chondroitin sulfate from fish bones and co-producing collagen peptides and biological calcium is through the following specific process steps:

[0045] (1) Cooking

[0046] Select fresh marine fish bones as raw materials, send them to a continuous cooking tank for cooking for 60 minutes, and separate them with high-pressure air to obtain fish bone protein and fish bones;

[0047] (2) Crushing and fermentation

[0048] Wash and pulverize the fish bones obtained in step (1), and control the average particle size to 0.1 mm to obtain fish bone powder; add the obtained fish bone powder to the same amount of pure water and stir evenly, and then inoculate the fish bone powder with a mass of 3% lactic acid bacteria, adjust the pH value to 6.0, ferment at a constant temperature, control the temperature at 50°C, and ferment for 12 hours to obtain a fermented liquid;

[0049] (3) Separation

[0050] centrifuging the fermented liquid obtained in step (2)...

Embodiment 3

[0061] The method for preparing chondroitin sulfate from fish bones and co-producing collagen peptides and biological calcium is through the following specific process steps:

[0062] (1) Cooking

[0063] Select fresh marine fish fish bones as raw materials, send them to a continuous cooking tank for cooking for 10 minutes, and separate them with high-pressure air to obtain fish bone protein and fish bones;

[0064] (2) Crushing and fermentation

[0065] Wash and pulverize the fish bones obtained in step (1), and control the average particle size to 0.5 mm to obtain fish bone powder; add the obtained fish bone powder to the same amount of pure water and stir evenly, and then inoculate the fish bone powder with a mass of 0.5% lactic acid bacteria, adjust the pH value to 5.0, ferment at a constant temperature, control the temperature at 35°C, and ferment for 48 hours to obtain a fermented liquid;

[0066] (3) Separation

[0067] Separating the fermented liquid obtained in ste...

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PUM

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Abstract

The invention relates to a method for preparing chondroitin sulfate and co-producing collagen peptide and biological calcium by using fishbone. The method comprises the following steps: by taking fishbone of marine fishes as raw material, stewing, crushing, inoculating with lactobacillus for fermentation and separating to obtain a clear solution and a precipitate; standing the clear solution at a low temperature; centrifuging; spray-drying and crushing the precipitate to obtain the biological calcium; washing the precipitate, and then performing enzymolysis, ultrafiltration concentration, anion exchange resin column chromatography, elution by deionized water and spray-drying to obtain the collagen peptide; eluting by using a NaCl solution; ultra-filtration desalting; precipitating by using alcohol; oxidizing and decolorizing; drying at reduced pressure to obtain chondroitin sulfate. The preparation method disclosed by the invention is reasonable in process and mild in operation condition; the prepared materials and products are high in purity; high-efficiency comprehensive utilization of fishbone resources is realized; problems of emission of wastewater and environmental pollution are greatly reduced.

Description

Technical field Background technique [0002] [0003] [0004] Invention content [0005] [0006] [0007] [0008] [0010] [0011] [0012] [0013] [0014] [0015] [0016] [0017] [0018] [0019] [0020] Detailed ways Example 1 [0030] [0031] [0032] [0033] [0034] [0035] [0036] [0037] [0038] [0039] [0040] [0041] [0042] [0043] Example 2 [0044] [0045] [0046] [0047] [0048] [0049] [0050] [0051] [0052] [0053] (5) Estated enzymes, extinguishing enzymes [0054] Wash the sediments obtained in the step (3), add pure water and stir well, and then add a flavor protease of 1%of the sediment mass to enzymatic disintegration.Heat the enzymatic solution to 100 ° C, and the enzymatic activation is 30 minutes to get the enzyme disintegration solution of the enzyme; [0055] (6) Control of collagen peptide [0056] The enzymatic solution obtained from the steps (5) is used for ultrafiltration and concentration to obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12P21/06C12P3/00C07K14/78C07K1/36C07K1/34C07K1/18C08B37/08C01F11/18
Inventor 王本新李银塔刘建松
Owner RONGCHENG HONGYE OCEAN TECH