Method for extracting high-purity phospholipid from Antarctic krill oil

Abstract

The invention discloses a method for extracting high-purity phospholipid from Antarctic krill oil. The method comprises the following steps: mixing a low polarity solvent, ethanol and water at the volume ratio of 10:10:1, fully oscillating, mixing, standing and layering, wherein the upper layer refers to a low polarity solvent phase, and the lower layer refers to an ethanol phase; separating the upper low polarity solvent phase; dissolving the Antarctic krill oil in the ethanol phase prepared in the step (1); adding the prepared low polarity solvent phase into the Antarctic krill oil dissolved liquid for extracting, and fully mixing; standing or centrifuging, so that the mixed solution is layered, thereby obtaining the upper dark red low polarity extracting solution and the lower orange-yellow ethanol phase extracting solution; extracting the ethanol phase extracting solution by using the low polarity solvent phase until the low polarity solvent phase is nearly colorless, and finally obtaining the orange-yellow ethanol phase extracting solution; and performing reduction vaporization on the finally obtained ethanol phase extracting solution, thereby obtaining the orange-yellow cream, namely the Antarctic krill phospholipid. According to the method disclosed by the invention, the high-purity phospholipid is prepared from the Antarctic krill oil by utilizing the organic solvent extraction method, and the phospholipid content is 85-92 percent.

Description

technical field [0001] The invention belongs to the field of health products or chemical industry, and in particular relates to a method for extracting high-purity phospholipids from Antarctic krill oil. Background technique [0002] Antarctic krill lipids are rich in omega-3 series polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA, 20:5) and docosahexaenoic acid (DHA, 22:6) 15.0%-25.3%, 9.3%-21.2% of the total fatty acid content. Studies have shown that the omega-3 series polyunsaturated fatty acids of Antarctic krill mainly exist in phospholipids. Compared with traditional glycerolipid fish oils, Antarctic krill phospholipids have better absorption in the human body and have more good physiological activity. [0003] The content of phospholipid (PL) in Antarctic krill lipid reaches about 40%, which is the only phospholipid resource with large-scale development potential in marine organisms. Phospholipids can be divided into phosphoglycerides and sphingo...

AI Technical Summary

Problems solved by technology

However, due to the phospholipid content in Antarctic krill oil reaches about 40%, the preliminary separation of phospholipids cannot be achieved by hydration.

Existing technology uses supercritical CO 2 The extraction method can effectively separate glycerolipids, cholesterol, etc. from Antarctic krill phospholipids, but the equipment required for production is expensive and consumes a lot of energy

In addition, the existing technology uses acetone extraction to separate Antarctic krill oil, and obtains acetone-insoluble matter as phospholipids. However, lecithin (PC), an important component of Antarctic krill phospholipids, can be dissolved in acetone, so it is impossible to effectively prepare high-quality Antarctic krill oil. Shrimp Phospholipids

Although column chromatography can effectively separate Antarctic krill oil phospholipids, the elution recovery rate will be affected due to the strong adsorption of phospholipids and stationary phase materials.